{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T18:08:05Z","timestamp":1790791685914,"version":"4.1.0"},"reference-count":43,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2009,6,1]],"date-time":"2009-06-01T00:00:00Z","timestamp":1243814400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2009,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p><jats:italic>Capnocytophaga canimorsus<\/jats:italic>is a bacterium of the canine oral flora known since 1976 to cause rare but severe septicemia and peripheral gangrene in patients that have been in contact with a dog. It was recently shown that these bacteria do not elicit an inflammatory response (H. Shin, M. Mally, M. Kuhn, C. Paroz, and G. R. Cornelis, J. Infect. Dis. 195:375-386, 2007). Here, we analyze their sensitivity to the innate immune system. Bacteria from the archetype strain Cc5 were highly resistant to killing by complement. There was little membrane attack complex (MAC) deposition in spite of C3b deposition. Cc5 bacteria were as resistant to phagocytosis by human polymorphonuclear leukocytes (PMNs) as<jats:italic>Yersinia enterocolitica<\/jats:italic>MRS40, endowed with an antiphagocytic type III secretion system. We isolated Y1C12, a transposon mutant that is hypersensitive to killing by complement via the antibody-dependent classical pathway. The mutation inactivated a putative glycosyltransferase gene, suggesting that the Y1C12 mutant was affected at the level of a capsular polysaccharide or lipopolysaccharide (LPS) structure. Cc5 appeared to have several polysaccharidic structures, one being altered in Y1C12. The structure missing in Y1C12 could be purified by classical LPS purification procedures and labeled by tritiated palmitate, indicating that it is more likely to be an LPS structure than a capsule. Y1C12 bacteria were also more sensitive to phagocytosis by PMNs than wild-type bacteria. In conclusion, a polysaccharide structure, likely an LPS, protects<jats:italic>C. canimorsus<\/jats:italic>from deposition of the complement MAC and from efficient phagocytosis by PMNs.<\/jats:p>","DOI":"10.1128\/iai.01324-08","type":"journal-article","created":{"date-parts":[[2009,3,24]],"date-time":"2009-03-24T00:55:34Z","timestamp":1237856134000},"page":"2262-2271","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":51,"title":["Resistance of<i>Capnocytophaga canimorsus<\/i>to Killing by Human Complement and Polymorphonuclear Leukocytes"],"prefix":"10.1128","volume":"77","author":[{"given":"Hwain","family":"Shin","sequence":"first","affiliation":[{"name":"Infection Biology, Biozentrum, University of Basel, Klingelbergstrasse 50\/70, CH-4056 Basel, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manuela","family":"Mally","sequence":"additional","affiliation":[{"name":"Infection Biology, Biozentrum, University of Basel, Klingelbergstrasse 50\/70, CH-4056 Basel, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Salome","family":"Meyer","sequence":"additional","affiliation":[{"name":"Infection Biology, Biozentrum, University of Basel, Klingelbergstrasse 50\/70, CH-4056 Basel, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chantal","family":"Fiechter","sequence":"additional","affiliation":[{"name":"Infection Biology, Biozentrum, University of Basel, Klingelbergstrasse 50\/70, CH-4056 Basel, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ce\u0301cile","family":"Paroz","sequence":"additional","affiliation":[{"name":"Infection Biology, Biozentrum, University of Basel, Klingelbergstrasse 50\/70, CH-4056 Basel, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ulrich","family":"Zaehringer","sequence":"additional","affiliation":[{"name":"Leibnitz Zentrum fu\u0308r Medizin und Biowissenschaften, Forschungszentrum Borstel, Parkallee 1-40, D-23845 Borstel, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guy R.","family":"Cornelis","sequence":"additional","affiliation":[{"name":"Infection Biology, Biozentrum, University of Basel, Klingelbergstrasse 50\/70, CH-4056 Basel, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.7.2.223-231.1978"},{"key":"e_1_3_2_3_2","first-page":"134","volume":"36","year":"1998","unstructured":"Blanche, P., E. 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