{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T02:33:02Z","timestamp":1774924382500,"version":"3.50.1"},"reference-count":26,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2010,6,1]],"date-time":"2010-06-01T00:00:00Z","timestamp":1275350400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2010,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:italic>Blastomyces dermatitidis<\/jats:italic>\n            is a thermally induced dimorphic fungus capable of causing lung and systemic infections in immunocompetent animal hosts. With the publication of genomic sequences from three different strains of\n            <jats:italic>B<\/jats:italic>\n            .\n            <jats:italic>dermatitidis<\/jats:italic>\n            and the development of RNA interference as a gene-silencing tool, it has become possible to easily ascertain the virulence and morphological effects of knocking down the expression of candidate genes of interest.\n            <jats:italic>BYS1<\/jats:italic>\n            (\n            <jats:italic>Blastomyces<\/jats:italic>\n            yeast-phase-specific 1), first identified by Burg and Smith, is expressed at high levels in yeast cells and is undetectable in mold. The deduced protein sequence of\n            <jats:italic>BYS1<\/jats:italic>\n            has a putative signal sequence at its N terminus, opening the possibility that the\n            <jats:italic>BYS1<\/jats:italic>\n            -encoded protein is associated with the yeast cell wall. Herein, strains of\n            <jats:italic>B<\/jats:italic>\n            .\n            <jats:italic>dermatitidis<\/jats:italic>\n            with silenced expression of\n            <jats:italic>BYS1<\/jats:italic>\n            were engineered and tested for morphology and virulence. The silenced strains produced rough-surfaced cultures on agar medium and demonstrated a propensity to form pseudohyphal cells on prolonged culture\n            <jats:italic>in vitro<\/jats:italic>\n            and\n            <jats:italic>in vivo<\/jats:italic>\n            , as measured in the mouse lung. Tests using a mouse model of blastomycosis with either yeast or spore inocula showed that the\n            <jats:italic>bys1<\/jats:italic>\n            -silenced strains were as virulent as control strains. Thus, although silencing of\n            <jats:italic>BYS1<\/jats:italic>\n            alters morphology at 37\u00b0C, it does not appear to impair the pathogenicity of\n            <jats:italic>B<\/jats:italic>\n            .\n            <jats:italic>dermatitidis<\/jats:italic>\n            .\n          <\/jats:p>","DOI":"10.1128\/iai.01328-09","type":"journal-article","created":{"date-parts":[[2010,4,6]],"date-time":"2010-04-06T00:26:28Z","timestamp":1270513588000},"page":"2522-2528","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":11,"title":["Discordant Influence of\n            <i>Blastomyces dermatitidis<\/i>\n            Yeast-Phase-Specific Gene\n            <i>BYS1<\/i>\n            on Morphogenesis and Virulence"],"prefix":"10.1128","volume":"78","author":[{"given":"Theerapong","family":"Krajaejun","sequence":"first","affiliation":[{"name":"Departments of Pediatrics"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcel","family":"Wu\u0308thrich","sequence":"additional","affiliation":[{"name":"Departments of Pediatrics"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gregory M.","family":"Gauthier","sequence":"additional","affiliation":[{"name":"Medicine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas F.","family":"Warner","sequence":"additional","affiliation":[{"name":"Pathology and Laboratory Medicine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas D.","family":"Sullivan","sequence":"additional","affiliation":[{"name":"Departments of Pediatrics"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bruce S.","family":"Klein","sequence":"additional","affiliation":[{"name":"Departments of Pediatrics"},{"name":"Medicine"},{"name":"Pathology and Laboratory Medicine"},{"name":"Medical Microbiology and Immunology, School of Medicine and Public Health, University of Wisconsin, Madison, Wisconsin 53706"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-7-29"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00304-07"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/EC.4.4.685-693.2005"},{"key":"e_1_3_2_5_2","first-page":"113","volume":"152","year":"2001","unstructured":"Bono, J. L., B. Jaber, M. A. Fisher, R. O. Abuodeh, E. O'Leary-Jepson, G. M. Scalarone, and L. H. Smith, Jr. 2001. Genetic diversity and transcriptional analysis of the bys1 gene from Blastomyces dermatitidis. Mycopathologia152:113-123.","journal-title":"Mycopathologia"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.275.11.7925"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2003.03415.x"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.189.8.1207"},{"key":"e_1_3_2_9_2","first-page":"4.9.1","year":"1997","unstructured":"Brown, T., and K. Mackey. 1997. Analysis of RNA by Northern and slot blot hybridization, p. 4.9.1-4.9.16. In F. M. Ausubel, R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, and J. A. Smith (ed.), Current protocols in molecular biology. 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