{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,27]],"date-time":"2026-01-27T14:54:42Z","timestamp":1769525682379,"version":"3.49.0"},"reference-count":46,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2013,6,1]],"date-time":"2013-06-01T00:00:00Z","timestamp":1370044800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2013,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Malaria is one of the most important infectious diseases in the world, and it has many economic and social impacts on populations, especially in poor countries. Transmission-blocking vaccines (TBVs) are valuable tools for malaria eradication. A study on\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Anopheles gambiae<\/jats:named-content>\n            revealed that polyclonal antibodies to carboxypeptidase B1 of\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">A. gambiae<\/jats:named-content>\n            can block sexual parasite development in the mosquito midgut. Hence, it was introduced as a TBV target in regions where\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">A. gambiae<\/jats:named-content>\n            is the main malaria vector. However, in Iran and neighboring countries as far as China, the main malaria vector is\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Anopheles stephensi<\/jats:named-content>\n            . Also, the genome of this organism has not been sequenced yet. Therefore, in this study, carboxypeptidase B1 of\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">A. stephensi<\/jats:named-content>\n            was characterized by genomic and proteomic approaches. Furthermore, its expression pattern after ingestion of\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Plasmodium falciparum<\/jats:named-content>\n            gametocytes and the effect of anti-CPBAs1 antibodies on sexual parasite development were evaluated. Our results revealed that the\n            <jats:italic>cpbAs1<\/jats:italic>\n            expression level was increased after ingestion of the mature gametocytes of\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">P. falciparum<\/jats:named-content>\n            and that anti-CPBAs1 directed antibodies could significantly reduce the mosquito infection rate in the test group compared with the control group. Therefore, according to our findings and with respect to the high similarity of carboxypeptidase enzymes between the two main malaria vectors in Africa (\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">A. gambiae<\/jats:named-content>\n            ) and Asia (\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">A. stephensi<\/jats:named-content>\n            ) and the presence of other sympatric vectors, CPBAs1 could be introduced as a TBV candidate in regions where\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">A. stephensi<\/jats:named-content>\n            is the main malaria vector, and this will broaden the scope for the potential wider application of CPBAs1 antigen homologs\/orthologs.\n          <\/jats:p>","DOI":"10.1128\/iai.01331-12","type":"journal-article","created":{"date-parts":[[2013,4,9]],"date-time":"2013-04-09T02:22:40Z","timestamp":1365474160000},"page":"2206-2216","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":39,"title":["Molecular Characterization of the Carboxypeptidase B1 of Anopheles stephensi and Its Evaluation as a Target for Transmission-Blocking Vaccines"],"prefix":"10.1128","volume":"81","author":[{"given":"Abbasali","family":"Raz","sequence":"first","affiliation":[{"name":"Malaria and Vector Research Group (MVRG), Biotechnology Research Center (BRC), Pasteur Institute of Iran, Tehran, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Navid","family":"Dinparast Djadid","sequence":"additional","affiliation":[{"name":"Malaria and Vector Research Group (MVRG), Biotechnology Research Center (BRC), Pasteur Institute of Iran, Tehran, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sedigheh","family":"Zakeri","sequence":"additional","affiliation":[{"name":"Malaria and Vector Research Group (MVRG), Biotechnology Research Center (BRC), Pasteur Institute of Iran, Tehran, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.1000398"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.65.3.1109-1113.1997"},{"key":"e_1_3_2_4_2","first-page":"118","article-title":"Development of malaria vaccines that block transmission of parasites by mosquito vectors","volume":"49","author":"Hisaeda H","year":"2002","unstructured":"HisaedaHYasutomoK. 2002. 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