{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T02:03:05Z","timestamp":1791424985992,"version":"4.3.3"},"reference-count":67,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2009,6,1]],"date-time":"2009-06-01T00:00:00Z","timestamp":1243814400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2009,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            A new\n            <jats:italic>Escherichia coli<\/jats:italic>\n            virulent clonal group, O45:K1, belonging to the highly virulent subgroup B2\n            <jats:sub>1<\/jats:sub>\n            was recently identified in France, where it accounts for one-third of\n            <jats:italic>E. coli<\/jats:italic>\n            neonatal meningitis cases. Here we describe the sequence, epidemiology and function of the large plasmid harbored by strain S88, which is representative of the O45:K1 clonal group. Plasmid pS88 is 133,853 bp long and contains 144 protein-coding genes. It harbors three different iron uptake systems (aerobactin, salmochelin, and the\n            <jats:italic>sitABCD<\/jats:italic>\n            genes) and other putative virulence genes (\n            <jats:italic>iss<\/jats:italic>\n            ,\n            <jats:italic>etsABC<\/jats:italic>\n            ,\n            <jats:italic>ompT<\/jats:italic>\n            <jats:sub>P<\/jats:sub>\n            , and\n            <jats:italic>hlyF<\/jats:italic>\n            ). The pS88 sequence is composed of several gene blocks homologous to avian pathogenic\n            <jats:italic>E. coli<\/jats:italic>\n            plasmids pAPEC-O2-ColV and pAPEC-O1-ColBM. PCR amplification of 11 open reading frames scattered throughout the plasmid was used to investigate the distribution of pS88 and showed that a pS88-like plasmid is present in other meningitis clonal groups such as O18:K1, O1:K1, and O83:K1. A pS88-like plasmid was also found in avian pathogenic strains and human urosepsis strains belonging to subgroup B2\n            <jats:sub>1<\/jats:sub>\n            . A variant of S88 cured of its plasmid displayed a marked loss of virulence relative to the wild-type strain in a neonatal rat model, with bacteremia more than 2 log CFU\/ml lower. The salmochelin siderophore, a known meningovirulence factor, could not alone explain the plasmid's contribution to virulence, as a salmochelin mutant displayed only a minor fall in bacteremia (0.9 log CFU\/ml). Thus, pS88 is a major virulence determinant related to avian pathogenic plasmids that has spread not only through meningitis clonal groups but also human urosepsis and avian pathogenic strains.\n          <\/jats:p>","DOI":"10.1128\/iai.01333-08","type":"journal-article","created":{"date-parts":[[2009,3,24]],"date-time":"2009-03-24T00:55:34Z","timestamp":1237856134000},"page":"2272-2284","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":129,"title":["The Plasmid of\n            <i>Escherichia coli<\/i>\n            Strain S88 (O45:K1:H7) That Causes Neonatal Meningitis Is Closely Related to Avian Pathogenic\n            <i>E. coli<\/i>\n            Plasmids and Is Associated with High-Level Bacteremia in a Neonatal Rat Meningitis Model"],"prefix":"10.1128","volume":"77","author":[{"given":"Chantal","family":"Peigne","sequence":"first","affiliation":[{"name":"Laboratoire d'E\u0301tudes de Ge\u0301ne\u0301tique Bacte\u0301rienne dans les Infections de l'Enfant (EA 3105), Universite\u0301 Paris Diderot, Ho\u0302pital Robert Debre\u0301, 75019 Paris"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippe","family":"Bidet","sequence":"additional","affiliation":[{"name":"Laboratoire d'E\u0301tudes de Ge\u0301ne\u0301tique Bacte\u0301rienne dans les Infections de l'Enfant (EA 3105), Universite\u0301 Paris Diderot, Ho\u0302pital Robert Debre\u0301, 75019 Paris"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Farah","family":"Mahjoub-Messai","sequence":"additional","affiliation":[{"name":"Laboratoire d'E\u0301tudes de Ge\u0301ne\u0301tique Bacte\u0301rienne dans les Infections de l'Enfant (EA 3105), Universite\u0301 Paris Diderot, Ho\u0302pital Robert Debre\u0301, 75019 Paris"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ce\u0301line","family":"Plainvert","sequence":"additional","affiliation":[{"name":"Laboratoire d'E\u0301tudes de Ge\u0301ne\u0301tique Bacte\u0301rienne dans les Infections de l'Enfant (EA 3105), Universite\u0301 Paris Diderot, Ho\u0302pital Robert Debre\u0301, 75019 Paris"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vale\u0301rie","family":"Barbe","sequence":"additional","affiliation":[{"name":"Commissariat a\u0300 l'Energie Atomique, Direction des Sciences du Vivant, Institut de Ge\u0301nomique, Ge\u0301noscope, 91057 Evry Cedex"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudine","family":"Me\u0301digue","sequence":"additional","affiliation":[{"name":"Commissariat a\u0300 l'Energie Atomique, Direction des Sciences du Vivant, Institut de Ge\u0301nomique, Atelier de Ge\u0301nomique Comparative, CNRS UMR8030, 91057 Evry Cedex"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric","family":"Frapy","sequence":"additional","affiliation":[{"name":"Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale U570 and Universite\u0301 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Paris"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ste\u0301phane","family":"Bonacorsi","sequence":"additional","affiliation":[{"name":"Laboratoire d'E\u0301tudes de Ge\u0301ne\u0301tique Bacte\u0301rienne dans les Infections de l'Enfant (EA 3105), Universite\u0301 Paris Diderot, Ho\u0302pital Robert Debre\u0301, 75019 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Steinr\u00fcck, P. Buchholz, R. Prager, E. Tietze, G. Seltmann, and J. Hacker. 1990. Molecular analysis of Escherichia coli from neonatal infections and its epidemiological implication, p. 224-234. In E. Gravel, L. Stern, I. Syllim-Rapoport, and R. Waver (ed.), Research in perinatal medicine II. 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