{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T08:40:17Z","timestamp":1791189617062,"version":"4.1.0"},"reference-count":57,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2008,5,1]],"date-time":"2008-05-01T00:00:00Z","timestamp":1209600000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2008,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:italic>Burkholderia cenocepacia<\/jats:italic>\n            is an important opportunistic pathogen causing serious chronic infections in patients with cystic fibrosis (CF). Adaptation of\n            <jats:italic>B. cenocepacia<\/jats:italic>\n            to the CF airways may play an important role in the persistence of the infection. We have identified a sensor kinase-response regulator (BCAM0379) named AtsR in\n            <jats:italic>B. cenocepacia<\/jats:italic>\n            K56-2 that shares 19% amino acid identity with RetS from\n            <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n            .\n            <jats:italic>atsR<\/jats:italic>\n            inactivation led to increased biofilm production and a hyperadherent phenotype in both abiotic surfaces and lung epithelial cells. Also, the\n            <jats:italic>atsR<\/jats:italic>\n            mutant overexpressed and hypersecreted an Hcp-like protein known to be specifically secreted by the type VI secretion system (T6SS) in other gram-negative bacteria. Amoeba plaque assays demonstrated that the\n            <jats:italic>atsR<\/jats:italic>\n            mutant was more resistant to\n            <jats:italic>Dictyostelium<\/jats:italic>\n            predation than the wild-type strain and that this phenomenon was T6SS dependent. Macrophage infection assays also demonstrated that the\n            <jats:italic>atsR<\/jats:italic>\n            mutant induces the formation of actin-mediated protrusions from macrophages that require a functional Hcp-like protein, suggesting that the T6SS is involved in actin rearrangements. Three\n            <jats:italic>B. cenocepacia<\/jats:italic>\n            transposon mutants that were found in a previous study to be impaired for survival in chronic lung infection model were mapped to the T6SS gene cluster, indicating that the T6SS is required for infection in vivo. Together, our data show that AtsR is involved in the regulation of genes required for virulence in\n            <jats:italic>B. cenocepacia<\/jats:italic>\n            K56-2, including genes encoding a T6SS.\n          <\/jats:p>","DOI":"10.1128\/iai.01338-07","type":"journal-article","created":{"date-parts":[[2008,3,4]],"date-time":"2008-03-04T01:35:04Z","timestamp":1204594504000},"page":"1979-1991","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":117,"title":["A Novel Sensor Kinase-Response Regulator Hybrid Controls Biofilm Formation and Type VI Secretion System Activity in\n            <i>Burkholderia cenocepacia<\/i>"],"prefix":"10.1128","volume":"76","author":[{"given":"Daniel F.","family":"Aubert","sequence":"first","affiliation":[{"name":"Infectious Diseases Research Group, Departments of Microbiology and Immunology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ronald S.","family":"Flannagan","sequence":"additional","affiliation":[{"name":"Infectious Diseases Research Group, Departments of Microbiology and Immunology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miguel A.","family":"Valvano","sequence":"additional","affiliation":[{"name":"Infectious Diseases Research Group, Departments of Microbiology and Immunology"},{"name":"Medicine, Siebens-Drake Research Institute, University of Western Ontario, London, Ontario, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkh121"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1104\/pp.117.3.723"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1462-2920.2003.00471.x"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.69.8.2110"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.26243-0"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.184.11.3027-3033.2002"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/81.19.1492"},{"key":"e_1_3_2_9_2","first-page":"287","volume":"3","year":"2003","unstructured":"Das, S., and K. 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