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The\n                    <jats:italic>B. mallei<\/jats:italic>\n                    VirAG two-component regulatory system activates the transcription of \u223c60 genes, including a large virulence gene cluster encoding a type VI secretion system (T6SS). The\n                    <jats:italic>B. mallei tssM<\/jats:italic>\n                    gene encodes a putative ubiquitin-specific protease that is physically linked to, and transcriptionally coregulated with, the T6SS gene cluster. Mass spectrometry and immunoblot analysis demonstrated that TssM was secreted in a\n                    <jats:italic>virAG<\/jats:italic>\n                    -dependent manner in vitro. Surprisingly, the T6SS was found to be dispensable for the secretion of TssM. The C-terminal half of TssM, which contains Cys and His box motifs conserved in eukaryotic deubiquitinases, was purified and biochemically characterized. Recombinant TssM hydrolyzed multiple ubiquitinated substrates and the cysteine at position 102 was critical for enzymatic activity. The\n                    <jats:italic>tssM<\/jats:italic>\n                    gene was expressed within 1 h after uptake of\n                    <jats:italic>B. mallei<\/jats:italic>\n                    into RAW 264.7 murine macrophages, suggesting that the TssM deubiquitinase is produced in this intracellular niche. Although the physiological substrate(s) is currently unknown, the TssM deubiquitinase may provide\n                    <jats:italic>B. mallei<\/jats:italic>\n                    a selective advantage in the intracellular environment during infection.\n                  <\/jats:p>","DOI":"10.1128\/iai.01339-08","type":"journal-article","created":{"date-parts":[[2009,1,23]],"date-time":"2009-01-23T21:41:02Z","timestamp":1232746862000},"page":"1636-1648","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":51,"title":["<i>Burkholderia mallei tssM<\/i>\n                    Encodes a Putative Deubiquitinase That Is Secreted and Expressed inside Infected RAW 264.7 Murine Macrophages"],"prefix":"10.1128","volume":"77","author":[{"given":"John","family":"Shanks","sequence":"first","affiliation":[{"name":"Department of Biochemistry, Emory University School of Medicine, Atlanta, Georgia 30322"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mary N.","family":"Burtnick","sequence":"additional","affiliation":[{"name":"Laboratory of Zoonotic Pathogens, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul J.","family":"Brett","sequence":"additional","affiliation":[{"name":"Laboratory of Zoonotic Pathogens, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David M.","family":"Waag","sequence":"additional","affiliation":[{"name":"Bacteriology Division, U.S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, Maryland 21702-5011"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin B.","family":"Spurgers","sequence":"additional","affiliation":[{"name":"Bacteriology Division, U.S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, Maryland 21702-5011"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wilson J.","family":"Ribot","sequence":"additional","affiliation":[{"name":"Bacteriology Division, U.S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, Maryland 21702-5011"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark A.","family":"Schell","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Georgia, Athens, Georgia 30602"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rekha G.","family":"Panchal","sequence":"additional","affiliation":[{"name":"Bacteriology Division, U.S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, Maryland 21702-5011"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank C.","family":"Gherardini","sequence":"additional","affiliation":[{"name":"Laboratory of Zoonotic Pathogens, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keith D.","family":"Wilkinson","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Emory University School of Medicine, Atlanta, Georgia 30322"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"DeShazer","sequence":"additional","affiliation":[{"name":"Bacteriology Division, U.S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, Maryland 21702-5011"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.17.3389"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2004.10.003"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.0030003"},{"key":"e_1_3_2_5_2","doi-asserted-by":"crossref","first-page":"727","DOI":"10.1515\/bchm.2001.382.5.727","volume":"382","year":"2001","unstructured":"Barrett, A. 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