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In doing this, pathogens\nevolved different type of transport systems to help them survive and\ndisseminate within the host. Members of the TolC family have been shown\nto be involved in the export of chemically diverse molecules ranging\nfrom large protein toxins to small toxic compounds. The role of\nproteins from the TolC family in\n            <jats:italic>Brucella<\/jats:italic>\n            and other\u03b1\n-2-proteobacteria has been explored little. The gene encoding\nthe unique member of the TolC family from\n            <jats:italic>Brucella suis<\/jats:italic>\n            (BepC)\nwas cloned and expressed in an\n            <jats:italic>Escherichia coli<\/jats:italic>\n            mutant\ndisrupted in the gene encoding TolC, which has the peculiarity of being\ninvolved in diverse transport functions. BepC fully complemented the\nresistance to drugs such as chloramphenicol and acriflavine but was\nincapable of restoring hemolysin secretion in the\n            <jats:italic>tolC<\/jats:italic>\n            mutant\nof\n            <jats:italic>E. coli<\/jats:italic>\n            . An insertional mutation in the\n            <jats:italic>bepC<\/jats:italic>\n            gene\nstrongly affected the resistance phenotype of\n            <jats:italic>B. suis<\/jats:italic>\n            to bile\nsalts and toxic chemicals such as ethidium bromide and rhodamine and\nsignificantly decreased the resistance to antibiotics such as\nerythromycin, ampicillin, tetracycline, and norfloxacin. Moreover, the\n            <jats:italic>B. suis bepC<\/jats:italic>\n            mutant was attenuated in the mouse model of\ninfection. Taken together, these results suggest that BepC-dependent\nefflux processes of toxic compounds contribute to\n            <jats:italic>B. suis<\/jats:italic>\n            survival inside the\nhost.\n          <\/jats:p>","DOI":"10.1128\/iai.01349-06","type":"journal-article","created":{"date-parts":[[2006,12,20]],"date-time":"2006-12-20T18:51:25Z","timestamp":1166640685000},"page":"379-389","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":47,"title":["The TolC Homologue of\n            <i>Brucella suis<\/i>\n            Is Involved in Resistance toAntimicrobial Compounds and Virulence"],"prefix":"10.1128","volume":"75","author":[{"given":"Diana M.","family":"Posadas","sequence":"first","affiliation":[{"name":"Fundacio\u0301n\rInstituto Leloir, CONICET and FCEyN, University of Buenos Aires,\rPatricias Argentinas 435, C1405BWE Buenos Aires,\rArgentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernando A.","family":"Marti\u0301n","sequence":"additional","affiliation":[{"name":"Fundacio\u0301n\rInstituto Leloir, CONICET and FCEyN, University of Buenos Aires,\rPatricias Argentinas 435, C1405BWE Buenos Aires,\rArgentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia V.","family":"Sabio y Garci\u0301a","sequence":"additional","affiliation":[{"name":"Instituto de\rBiotecnologi\u0301a, Centro de Investigaciones en Ciencias Veterinarias\ry Agrono\u0301micas, P.O. Box 77, 1708 Moro\u0301n,\rBuenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan M.","family":"Spera","sequence":"additional","affiliation":[{"name":"Instituto de\rInvestigaciones Biotecnolo\u0301gicas, Universidad de Gral. San\rMartin, Av. Gral. Paz 5445, INTI, Ed. 24, 1650 San\rMarti\u0301n, Buenos Aires,\rArgentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. Victoria","family":"Delpino","sequence":"additional","affiliation":[{"name":"Instituto de Estudios de la\rInmunidad Humoral, Facultad de Farmacia y Bioqui\u0301mica,\rUniversidad de Buenos Aires, Juni\u0301n 956, 4to. piso, 1113 Buenos\rAires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pablo","family":"Baldi","sequence":"additional","affiliation":[{"name":"Instituto de Estudios de la\rInmunidad Humoral, Facultad de Farmacia y Bioqui\u0301mica,\rUniversidad de Buenos Aires, Juni\u0301n 956, 4to. piso, 1113 Buenos\rAires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eleonora","family":"Campos","sequence":"additional","affiliation":[{"name":"Instituto de\rBiotecnologi\u0301a, Centro de Investigaciones en Ciencias Veterinarias\ry Agrono\u0301micas, P.O. 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