{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T01:30:45Z","timestamp":1793410245951,"version":"4.5.0"},"reference-count":83,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2016,5,1]],"date-time":"2016-05-01T00:00:00Z","timestamp":1462060800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["AI101982"],"award-info":[{"award-number":["AI101982"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"HHS | NIH | National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["GM085448"],"award-info":[{"award-number":["GM085448"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008065","name":"Georgia Research Alliance","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008065","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2016,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Infection of mammals by the parasitic helminth\n            <jats:named-content content-type=\"genus-species\">Schistosoma mansoni<\/jats:named-content>\n            induces antibodies to glycan antigens in worms and eggs, but the differential nature of the immune response among infected mammals is poorly understood. To better define these responses, we used a shotgun glycomics approach in which N-glycans from schistosome egg glycoproteins were prepared, derivatized, separated, and used to generate an egg shotgun glycan microarray. This array was interrogated with sera from infected mice, rhesus monkeys, and humans and with glycan-binding proteins and antibodies to gather information about the structures of antigenic glycans, which also were analyzed by mass spectrometry. A major glycan antigen targeted by IgG from different infected species is the FLDNF epitope [Fuc\u03b13GalNAc\u03b24(Fuc\u03b13)GlcNAc-R], which is also recognized by the IgG monoclonal antibody F2D2. The FLDNF antigen is expressed by all life stages of the parasite in mammalian hosts, and F2D2 can kill schistosomula\n            <jats:italic>in vitro<\/jats:italic>\n            in a complement-dependent manner. Different antisera also recognized other glycan determinants, including core \u03b2-xylose and highly fucosylated glycans. Thus, the natural shotgun glycan microarray of schistosome eggs is useful in identifying antigenic glycans and in developing new anti-glycan reagents that may have diagnostic applications and contribute to developing new vaccines against schistosomiasis.\n          <\/jats:p>","DOI":"10.1128\/iai.01349-15","type":"journal-article","created":{"date-parts":[[2016,2,17]],"date-time":"2016-02-17T03:58:22Z","timestamp":1455681502000},"page":"1371-1386","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":32,"title":["Identification of Antigenic Glycans from Schistosoma mansoni by Using a Shotgun Egg Glycan Microarray"],"prefix":"10.1128","volume":"84","author":[{"given":"Megan L.","family":"Mickum","sequence":"first","affiliation":[{"name":"Department of Biochemistry and the Emory Glycomics Center, Emory University School of Medicine, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nina Salinger","family":"Prasanphanich","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and the Emory Glycomics Center, Emory University School of Medicine, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuezheng","family":"Song","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and the Emory Glycomics Center, Emory University School of Medicine, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nelum","family":"Dorabawila","sequence":"additional","affiliation":[{"name":"Department of Natural Sciences, University of Maryland Eastern Shores, Princess Anne, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Msano","family":"Mandalasi","sequence":"additional","affiliation":[{"name":"Department of Natural Sciences, University of Maryland Eastern Shores, Princess Anne, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Lasanajak","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and the Emory Glycomics Center, Emory University School of Medicine, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anthony","family":"Luyai","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and the Emory Glycomics Center, Emory University School of Medicine, Atlanta, Georgia, USA"},{"name":"Centers for Disease Control and Prevention, Division of Parasitic Diseases and Malaria, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W. 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