{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,27]],"date-time":"2025-11-27T13:46:27Z","timestamp":1764251187742},"reference-count":37,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2008,4,1]],"date-time":"2008-04-01T00:00:00Z","timestamp":1207008000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2008,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>CD8<jats:sup>+<\/jats:sup>T cells play a pivotal role in protection against<jats:italic>Mycobacterium tuberculosis<\/jats:italic>infection. We identified a novel HLA-A*0201-restricted CD8<jats:sup>+<\/jats:sup>T-cell epitope on a dominant secreted antigen of<jats:italic>M. tuberculosis<\/jats:italic>, MPT51, in HLA-A*0201 transgenic HHD mice. HHD mice were immunized with plasmid DNA encoding MPT51 with gene gun bombardment, and gamma interferon (IFN-\u03b3) production by the immune splenocytes was analyzed. In response to overlapping synthetic peptides covering the mature MPT51 sequence, the splenocytes were stimulated to produce IFN-\u03b3 by only one peptide, p51-70. Three-color flow cytometric analysis of intracellular IFN-\u03b3 and cell surface CD4 and CD8 staining revealed that the MPT51 p51-70 peptide contains an immunodominant CD8<jats:sup>+<\/jats:sup>T-cell epitope. Further analysis using computer algorithms permitted identification of a bona fide T-cell epitope, p53-62. A major histocompatibility complex class I stabilization assay using T2 cells confirmed that this epitope binds to HLA-A*0201. The T cells were capable of lysing MPT51 p53-62 peptide-pulsed T2 cells. In addition, MPT51 p53-62-specific memory CD8<jats:sup>+<\/jats:sup>T cells were found in tuberculin skin test-positive HLA-A*0201<jats:sup>+<\/jats:sup>healthy individuals. Use of this HLA-A*0201-restricted CD8<jats:sup>+<\/jats:sup>T-cell epitope for analysis of the role of MPT51-specific T cells in<jats:italic>M. tuberculosis<\/jats:italic>infection and for design of vaccines against tuberculosis is feasible.<\/jats:p>","DOI":"10.1128\/iai.01381-07","type":"journal-article","created":{"date-parts":[[2008,1,23]],"date-time":"2008-01-23T01:44:47Z","timestamp":1201052687000},"page":"1565-1571","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":20,"title":["Identification of an HLA-A*0201-Restricted T-Cell Epitope on the MPT51 Protein, a Major Secreted Protein Derived from<i>Mycobacterium tuberculosis<\/i>, by MPT51 Overlapping Peptide Screening"],"prefix":"10.1128","volume":"76","author":[{"given":"Taiki","family":"Aoshi","sequence":"first","affiliation":[{"name":"Department of Infectious Diseases"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toshi","family":"Nagata","sequence":"additional","affiliation":[{"name":"Department of Health Science"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mina","family":"Suzuki","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masato","family":"Uchijima","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dai","family":"Hashimoto","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Second Division, Hamamatsu University School of Medicine, Hamamatsu 431-3192, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alireza","family":"Rafiei","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takafumi","family":"Suda","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Second Division, Hamamatsu University School of Medicine, Hamamatsu 431-3192, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kingo","family":"Chida","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Second Division, Hamamatsu University School of Medicine, Hamamatsu 431-3192, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yukio","family":"Koide","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.56.12.3046-3051.1988"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.62.6.2536-2544.1994"},{"key":"e_1_3_2_4_2","doi-asserted-by":"crossref","first-page":"3359","DOI":"10.4049\/jimmunol.154.7.3359","volume":"154","year":"1995","unstructured":"Andersen, P., \u00c5. 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