{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T20:30:11Z","timestamp":1790281811329,"version":"4.1.0"},"reference-count":50,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2014,4,1]],"date-time":"2014-04-01T00:00:00Z","timestamp":1396310400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2014,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Pneumolysin (Ply) and its variants are protective against pneumococcal infections in animal models, and as a Toll-like receptor 4 agonist, pneumolysin has been reported to be a mucosal adjuvant. DnaJ has been approved as a useful candidate vaccine protein; we therefore designed novel fusion proteins of DnaJ with a form of Ply that has a deletion of A146 (\u0394A146Ply-DnaJ [the C terminus of \u0394A146Ply connected with the N terminus of DnaJ] and DnaJ-\u0394A146Ply [the C terminus of DnaJ connected with the N terminus of \u0394A146Ply]) to test whether they are protective against focal and lethal pneumococcal infections and their potential protective mechanisms. The purified proteins were used to intranasally immunize the animals without additional adjuvant. Immunization with DnaJ-\u0394A146Ply or DnaJ plus \u0394A146Ply (Ply with a single deletion of A146) could significantly reduce\n            <jats:named-content content-type=\"genus-species\">S. pneumoniae<\/jats:named-content>\n            colonization in the nasopharynx and lung relative with DnaJ alone. Additionally, we observed the best protection for DnaJ-\u0394A146Ply-immunized mice after challenge with lethal doses of\n            <jats:named-content content-type=\"genus-species\">S. pneumoniae<\/jats:named-content>\n            strains, which was comparable to that achieved by PPV23. Mice immunized with DnaJ-\u0394A146Ply produced significantly higher levels of anti-DnaJ IgG in serum and secretory IgA (sIgA) in saliva than those immunized with DnaJ alone. The production of IL-17A was also striking in DnaJ-\u0394A146Ply-immunized mice. IL-17A knockout (KO) mice did not benefit from DnaJ-\u0394A146Ply immunization in colonization experiments, and sIgA production was impaired in IL-17A KO mice. Collectively, our results indicate a mucosal adjuvant potential for \u0394A146Ply and that, without additional adjuvant, DnaJ-\u0394A146Ply fusion protein exhibits extensive immune stimulation and is effective against pneumococcal challenges, properties which are partially attributed to the IL-17A-mediated immune responses.\n          <\/jats:p>","DOI":"10.1128\/iai.01391-13","type":"journal-article","created":{"date-parts":[[2014,2,4]],"date-time":"2014-02-04T03:45:09Z","timestamp":1391485509000},"page":"1666-1675","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":38,"title":["Mucosal Immunization with Recombinant Fusion Protein DnaJ-\u0394A146Ply Enhances Cross-Protective Immunity against Streptococcus pneumoniae Infection in Mice via Interleukin 17A"],"prefix":"10.1128","volume":"82","author":[{"given":"Yusi","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine, Key Laboratory 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