{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T20:07:41Z","timestamp":1791317261081,"version":"4.3.1"},"reference-count":39,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2010,8,1]],"date-time":"2010-08-01T00:00:00Z","timestamp":1280620800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2010,8]]},"abstract":"ABSTRACT<\/jats:title>\n \n Mycobacterium avium<\/jats:italic>\n subsp.\n paratuberculosis<\/jats:italic>\n , the agent of Johne's disease, infects ruminant hosts by translocation through the intestinal mucosa. A number of studies have suggested that\n M. avium<\/jats:italic>\n subsp.\n paratuberculosis<\/jats:italic>\n interacts with M cells in the Peyer's patches of the small intestine. The invasion of the intestinal mucosa by\n M. avium<\/jats:italic>\n subsp.\n paratuberculosis<\/jats:italic>\n and\n Mycobacterium avium<\/jats:italic>\n subsp.\n hominissuis<\/jats:italic>\n , a pathogen known to interact with intestinal cells, was compared.\n M. avium<\/jats:italic>\n subsp.\n paratuberculosis<\/jats:italic>\n was capable of invading the mucosa, but it was significantly less efficient at dissemination than\n M. avium<\/jats:italic>\n subsp.\n hominissuis<\/jats:italic>\n . B-cell knockout (KO) mice, which lack Peyer's patches, were used to demonstrate that\n M. avium<\/jats:italic>\n subsp.\n paratuberculosis<\/jats:italic>\n enters the intestinal mucosa through enterocytes in the absence of M cells. In addition, the results indicated that\n M. avium<\/jats:italic>\n subsp.\n paratuberculosis<\/jats:italic>\n had equal abilities to cross the mucosa in both Peyer's patch and non-Peyer's patch segments of normal mice.\n M. avium<\/jats:italic>\n subsp.\n paratuberculosis<\/jats:italic>\n was also shown to interact with epithelial cells by an \u03b1\n 5<\/jats:sub>\n \u03b2\n 1<\/jats:sub>\n integrin-independent pathway. Upon translocation, dendritic cells ingest\n M. avium<\/jats:italic>\n subsp.\n paratuberculosis<\/jats:italic>\n , but this process does not lead to efficient dissemination of the infection. In summary,\n M. avium<\/jats:italic>\n subsp.\n paratuberculosis<\/jats:italic>\n interacts with the intestinal mucosa by crossing both Peyer's patches and non-Peyer's patch areas but does not translocate or disseminate efficiently.\n <\/jats:p>","DOI":"10.1128\/iai.01411-09","type":"journal-article","created":{"date-parts":[[2010,5,24]],"date-time":"2010-05-24T22:23:54Z","timestamp":1274739834000},"page":"3570-3577","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":68,"title":["Peyer's Patch-Deficient Mice Demonstrate That\n Mycobacterium avium<\/i>\n subsp.\n paratuberculosis<\/i>\n Translocates across the Mucosal Barrier via both M Cells and Enterocytes but Has Inefficient Dissemination"],"prefix":"10.1128","volume":"78","author":[{"given":"Luiz E.","family":"Bermudez","sequence":"first","affiliation":[{"name":"Kuzell Institute, California Pacific Medical Center Research Institute, San Francisco, California 94115"},{"name":"Department of Biomedical Sciences, College of Veterinary Medicine"},{"name":"Department of Microbiology, College of Science, Oregon State University, Corvallis, Oregon 97331"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mary","family":"Petrofsky","sequence":"additional","affiliation":[{"name":"Kuzell Institute, California Pacific Medical Center Research Institute, San Francisco, California 94115"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandra","family":"Sommer","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, College of Veterinary Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rau\u0301l G.","family":"Barletta","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine and Biomedical Sciences, University of Nebraska, Lincoln, Nebraska 68583-0905"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01913-06"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/165.1.75"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.1.140-146.2002"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.62.5.2021-2026.1994"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.micro.58.030603.123726"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/151.1.179"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1086\/596033"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.286.5446.1965"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.57.1.225-230.1989"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.14.3.489-512.2001"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2007.03.009"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.6.3.266"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1099\/00222615-47-8-725"},{"key":"e_1_3_2_15_2","doi-asserted-by":"crossref","first-page":"1505","DOI":"10.1099\/00207713-52-5-1505","volume":"52","year":"2002","unstructured":"Mijs, W., P. de Haas, R. 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