{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T06:01:15Z","timestamp":1791180075213,"version":"4.1.0"},"reference-count":43,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2010,5,1]],"date-time":"2010-05-01T00:00:00Z","timestamp":1272672000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2010,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p><jats:italic>Brucella abortus<\/jats:italic>is a facultative intracellular bacterial pathogen that causes abortion in domestic animals and undulant fever in humans. The mechanism of virulence of<jats:italic>Brucella<\/jats:italic>spp. is not yet fully understood. Therefore, it is crucial to identify new molecules that can function as virulence factors to better understand the host-pathogen interplay. Herein, we identified the gene encoding the phosphoglycerate kinase (PGK) of<jats:italic>B. abortus<\/jats:italic>strain 2308. To test the role of PGK in<jats:italic>Brucella<\/jats:italic>pathogenesis, a<jats:italic>pgk<\/jats:italic>deletion mutant was constructed. Replacement of the wild-type<jats:italic>pgk<\/jats:italic>by recombination was demonstrated by Southern and Western blot analyses. The<jats:italic>B. abortus<\/jats:italic>\u0394<jats:italic>pgk<\/jats:italic>mutant strain exhibited extreme attenuation in bone marrow-derived macrophages and<jats:italic>in vivo<\/jats:italic>in BALB\/c, C57BL\/6, 129\/Sv, and interferon regulatory factor-1 knockout (IRF-1 KO) mice. Additionally, at 24 h postinfection the \u0394<jats:italic>pgk<\/jats:italic>mutant was not found within the same endoplasmic reticulum-derived compartment as the wild-type bacteria, but, instead, over 60% of<jats:italic>Brucella<\/jats:italic>-containing vacuoles (BCVs) retained the late endosomal\/lysosomal marker LAMP1. Furthermore, the<jats:italic>B. abortus<\/jats:italic>\u0394<jats:italic>pgk<\/jats:italic>deletion mutant was used as a live vaccine. Challenge experiments revealed that the \u0394<jats:italic>pgk<\/jats:italic>mutant strain induced protective immunity in 129\/Sv or IRF-1 KO mice that was superior to the protection conferred by commercial strain 19 or RB51. Finally, the results shown here demonstrated that<jats:italic>Brucella<\/jats:italic>PGK is critical for full bacterial virulence and that a \u0394<jats:italic>pgk<\/jats:italic>mutant may serve as a potential vaccine candidate in future studies.<\/jats:p>","DOI":"10.1128\/iai.01433-09","type":"journal-article","created":{"date-parts":[[2010,3,2]],"date-time":"2010-03-02T04:34:14Z","timestamp":1267504454000},"page":"2283-2291","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":37,"title":["The<i>Brucella abortus<\/i>Phosphoglycerate Kinase Mutant Is Highly Attenuated and Induces Protection Superior to That of Vaccine Strain 19 in Immunocompromised and Immunocompetent Mice"],"prefix":"10.1128","volume":"78","author":[{"given":"Cyntia G. M. C.","family":"Trant","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Immunology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thais L. 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