{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T19:32:37Z","timestamp":1780601557759,"version":"3.54.1"},"reference-count":43,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2013,4,1]],"date-time":"2013-04-01T00:00:00Z","timestamp":1364774400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2013,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Burkholderia pseudomallei<\/jats:named-content>\n            is a tier 1 select agent, and the causative agent of melioidosis, a disease with effects ranging from chronic abscesses to fulminant pneumonia and septic shock, which can be rapidly fatal. Autotransporters (ATs) are outer membrane proteins belonging to the type V secretion system family, and many have been shown to play crucial roles in pathogenesis. The open reading frame Bp1026b_II1054 (\n            <jats:italic>bcaA<\/jats:italic>\n            ) in\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">B. pseudomallei<\/jats:named-content>\n            strain 1026b is predicted to encode a classical autotransporter protein with an approximately 80-kDa passenger domain that contains a subtilisin-related domain. Immediately 3\u2032 to\n            <jats:italic>bcaA<\/jats:italic>\n            is Bp11026_II1055 (\n            <jats:italic>bcaB<\/jats:italic>\n            ), which encodes a putative prolyl 4-hydroxylase. To investigate the role of these genes in pathogenesis, large in-frame deletion mutations of\n            <jats:italic>bcaA<\/jats:italic>\n            and\n            <jats:italic>bcaB<\/jats:italic>\n            were constructed in strain Bp340, an efflux pump mutant derivative of the melioidosis clinical isolate 1026b. Comparison of Bp340\u0394\n            <jats:italic>bcaA<\/jats:italic>\n            and Bp340\u0394\n            <jats:italic>bcaB<\/jats:italic>\n            mutants to wild-type\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">B. pseudomallei<\/jats:named-content>\n            <jats:italic>in vitro<\/jats:italic>\n            demonstrated similar levels of adherence to A549 lung epithelial cells, but the mutant strains were defective in their ability to invade these cells and to form plaques. In a BALB\/c mouse model of intranasal infection, similar bacterial burdens were observed after 48 h in the lungs and liver of mice infected with Bp340\u0394\n            <jats:italic>bcaA<\/jats:italic>\n            , Bp340\u0394\n            <jats:italic>bcaB<\/jats:italic>\n            , and wild-type bacteria. However, significantly fewer bacteria were recovered from the spleen of Bp340\u0394\n            <jats:italic>bcaA<\/jats:italic>\n            -infected mice, supporting the idea of a role for this AT in dissemination or in survival in the passage from the site of infection to the spleen.\n          <\/jats:p>","DOI":"10.1128\/iai.01453-12","type":"journal-article","created":{"date-parts":[[2013,1,23]],"date-time":"2013-01-23T07:52:59Z","timestamp":1358927579000},"page":"1121-1128","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":18,"title":["Characterization of BcaA, a Putative Classical Autotransporter Protein in Burkholderia pseudomallei"],"prefix":"10.1128","volume":"81","author":[{"given":"Cristine G.","family":"Campos","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, University of North Carolina at Chapel Hill School of Medicine, Chapel Hill, North Carolina, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luke","family":"Borst","sequence":"additional","affiliation":[{"name":"North Carolina State University, College of Veterinary Medicine, Raleigh, North Carolina, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peggy A.","family":"Cotter","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of North Carolina at Chapel Hill School of Medicine, Chapel Hill, North Carolina, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0403302101"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0450.2004.00797.x"},{"key":"e_1_3_2_4_2","first-page":"262","article-title":"An account of the discovery of a hitherto undescribed infective disease occurring among the population of Ragoon","author":"Whitmore A","year":"1912","unstructured":"WhitmoreAKrisnaswamiCS. 1912. 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