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Prior to phagocytosis of host cells,<jats:italic>E. histolytica<\/jats:italic>induces apoptotic host cell death, using a mechanism that requires contact via an amebic galactose-specific lectin. However, lectin inhibition only partially blocks phagocytosis of already dead cells, implicating at least one additional receptor in phagocytosis. To identify receptors for engulfment of apoptotic cells, monoclonal antibodies against<jats:italic>E. histolytica<\/jats:italic>membrane antigens were screened for inhibition of phagocytosis. Of 43 antibodies screened, one blocked lectin-independent uptake of apoptotic cells, with &gt;90% inhibition at a dose of 20 \u03bcg\/ml (<jats:italic>P<\/jats:italic>&lt; 0.0003 versus control). The same antibody also inhibited adherence to apoptotic lymphocytes and, to a lesser extent, adherence to and killing of viable lymphocytes. The antigen recognized by the inhibitory antibody was purified by affinity chromatography and identified by liquid chromatography-mass spectrometry as the serine-rich<jats:italic>E. histolytica<\/jats:italic>protein (SREHP). Consistent with this, the inhibitory antibody bound to recombinant SREHP present in bacterial lysates on immunoblots. The SREHP is an abundant immunogenic surface protein of unclear function. The results of this unbiased antibody screen strongly implicate the SREHP as a participant in<jats:italic>E. histolytica<\/jats:italic>phagocytosis and suggest that it may play an important role in adherence to apoptotic cells.<\/jats:p>","DOI":"10.1128\/iai.01455-07","type":"journal-article","created":{"date-parts":[[2007,12,18]],"date-time":"2007-12-18T02:31:18Z","timestamp":1197945078000},"page":"959-966","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":53,"title":["Participation of the Serine-Rich<i>Entamoeba histolytica<\/i>Protein in Amebic Phagocytosis of Apoptotic Host Cells"],"prefix":"10.1128","volume":"76","author":[{"given":"Jose E.","family":"Teixeira","sequence":"first","affiliation":[{"name":"Departments of Medicine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christopher D.","family":"Huston","sequence":"additional","affiliation":[{"name":"Departments of Medicine"},{"name":"Microbiology and Molecular Genetics, University of Vermont College of Medicine, Burlington, Vermont 05405"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1006\/expr.2001.4652"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.6.3422-3430.2005"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1080\/00365516809168206"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.36.1.396-406.1982"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.151.1.184"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(95)54029-6"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1006\/expr.1993.1105"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(78)90144-X"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1999.01500.x"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.6.3073-3081.1999"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.47.3.236"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1972.21.895"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.11.4081-4090.2002"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.8.3748-3756.2003"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.2.964-972.2003"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1462-5822.2000.00085.x"},{"key":"e_1_3_2_18_2","first-page":"172","volume":"46","year":"1947","unstructured":"Jacobs, L. 1947. 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