{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T22:34:52Z","timestamp":1791153292465,"version":"4.1.0"},"reference-count":42,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2009,4,1]],"date-time":"2009-04-01T00:00:00Z","timestamp":1238544000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2009,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Paracoccidioidomycosis (PCM) is a systemic granulomatous disease caused by the dimorphic fungus\n            <jats:italic>Paracoccidioides brasiliensis<\/jats:italic>\n            . Anti-PCM vaccine formulations based on the secreted fungal cell wall protein (gp43) or the derived P10 sequence containing a CD4\n            <jats:sup>+<\/jats:sup>\n            T-cell-specific epitope have shown promising results. In the present study, we evaluated new anti-PCM vaccine formulations based on the intranasal administration of\n            <jats:italic>P. brasiliensis<\/jats:italic>\n            gp43 or the P10 peptide in combination with the\n            <jats:italic>Salmonella enterica<\/jats:italic>\n            FliC flagellin, an innate immunity agonist binding specifically to the Toll-like receptor 5, in a murine model. BALB\/c mice immunized with gp43 developed high-specific-serum immunoglobulin G1 responses and enhanced interleukin-4 (IL-4) and IL-10 levels. On the other hand, mice immunized with recombinant purified flagellins genetically fused with P10 at the central hypervariable domain, either flanked or not by two lysine residues, or the synthetic P10 peptide admixed with purified FliC elicited a prevailing Th1-type immune response based on lung cell-secreted type 1 cytokines. Mice immunized with gp43 and FliC and intratracheally challenged with\n            <jats:italic>P. brasiliensis<\/jats:italic>\n            yeast cells had increased fungal proliferation and lung tissue damage. In contrast, mice immunized with the chimeric flagellins and particularly those immunized with P10 admixed with FliC reduced\n            <jats:italic>P. brasiliensis<\/jats:italic>\n            growth and lung damage. Altogether, these results indicate that\n            <jats:italic>S. enterica<\/jats:italic>\n            FliC flagellin modulates the immune response to\n            <jats:italic>P. brasiliensis<\/jats:italic>\n            P10 antigen and represents a promising alternative for the generation of anti-PCM vaccines.\n          <\/jats:p>","DOI":"10.1128\/iai.01470-08","type":"journal-article","created":{"date-parts":[[2009,2,10]],"date-time":"2009-02-10T01:54:20Z","timestamp":1234230860000},"page":"1700-1707","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":47,"title":["<i>Paracoccidioides brasiliensis<\/i>\n            Vaccine Formulations Based on the gp43-Derived P10 Sequence and the\n            <i>Salmonella enterica<\/i>\n            FliC Flagellin"],"prefix":"10.1128","volume":"77","author":[{"given":"Catarina J. M.","family":"Braga","sequence":"first","affiliation":[{"name":"Departamento de Microbiologia, Universidade de Sa\u0303o Paulo, Sa\u0303o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Glauce M. G.","family":"Rittner","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologia, Universidade de Sa\u0303o Paulo, Sa\u0303o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julian E.","family":"Mun\u0303oz Henao","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologia, Universidade de Sa\u0303o Paulo, Sa\u0303o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aline F.","family":"Teixeira","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologia, Universidade de Sa\u0303o Paulo, Sa\u0303o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liliana M.","family":"Massis","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologia, Universidade de Sa\u0303o Paulo, Sa\u0303o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria E.","family":"Sbrogio-Almeida","sequence":"additional","affiliation":[{"name":"Divisa\u0303o de Desenvolvimento Tecnolo\u0301gico e Produc\u0327a\u0303o, Instituto Butantan, Sa\u0303o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos P.","family":"Taborda","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologia, Universidade de Sa\u0303o Paulo, Sa\u0303o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luiz R.","family":"Travassos","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de Sa\u0303o Paulo, Sa\u0303o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lui\u0301s C. S.","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologia, Universidade de Sa\u0303o Paulo, Sa\u0303o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nri1391"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2006.02.015"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1590\/S1517-83822008000100011"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0966-842X(01)02292-2"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.6.2.89"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00349-08"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11046-007-9048-1"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.2.800-806.1998"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.5.2810-2816.2004"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2007.09.011"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.167.4.1882"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/35074106"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.74.2.1113-1120.2006"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2006.08.013"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2007.10.062"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF03402131"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00458-06"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni1112"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.74.1.694-702.2006"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1078\/0171-2985-00286"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1080\/13693780801982762"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.169.7.3914"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.2468182"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.2174\/1389557013406666"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.4.2369-2378.2004"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.10.6763-6770.2005"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(00)00074-8"},{"key":"e_1_3_2_30_2","first-page":"507","year":"1994","unstructured":"Puccia, R., L. R. Travassos, E. G. Rodrigues, A. K. Carmona, M. C. Oliveira, and L. Juliano. 1994. Purification of the specific exocellular antigen gp43 from Paracoccidioides brasiliensis: immunological and proteolytic activities, p. 507-515. In B. Maresca and G. S. Kobayashi (ed.), Molecular biology of pathogenic fungi: a laboratory manual. Telos Press, New York, NY.","journal-title":"Molecular biology of pathogenic fungi: a laboratory manual"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2004.09.002"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.18.1.190"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1006\/cyto.2002.0884"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1080\/02681219280000771"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.5.2546-2555.2004"},{"key":"e_1_3_2_36_2","first-page":"261","volume":"30","year":"1992","unstructured":"Singer-Vermes, L. M., M. C. Ciavaglia, S. S. Kashino, E. Burger, and V. L. Calich. 1992. The source of the growth-promoting factor(s) affects the plating efficiency of Paracoccidioides brasiliensis. J. Med. Vet. Mycol.30:261-264.","journal-title":"J. Med. Vet. Mycol."},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2249.2004.02507.x"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.2.786-793.1998"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.0300-9475.2004.01359.x"},{"key":"e_1_3_2_40_2","first-page":"241","year":"2007","unstructured":"Travassos, L. R., G. Goldman, C. P. Taborda, and R. Puccia. 2007. Insights in Paracoccidioides brasiliensis pathogenicity, p. 241-265. In K. Kavanagh (ed.), New insights in medical mycology. Springer, Dordrecht, The Netherlands.","journal-title":"New insights in medical mycology"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1586\/14787210.6.2.251"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11046-007-9056-1"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1016\/0264-410X(95)93308-V"}],"container-title":["Infection and Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.01470-08","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.01470-08","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T07:57:20Z","timestamp":1646467040000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/IAI.01470-08"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,4]]},"references-count":42,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2009,4]]}},"alternative-id":["10.1128\/IAI.01470-08"],"URL":"https:\/\/doi.org\/10.1128\/iai.01470-08","relation":{},"ISSN":["0019-9567","1098-5522"],"issn-type":[{"value":"0019-9567","type":"print"},{"value":"1098-5522","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,4]]},"assertion":[{"value":"2008-12-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2009-01-31","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2009-04-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}