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In infected target cells,<jats:named-content content-type=\"genus-species\">C. trachomatis<\/jats:named-content>replicates within parasitophorous vacuoles and expresses the protease-like activity factor CPAF. Previous studies have suggested that CPAF degrades the host transcription factors RFX5 and NF-\u03baB p65, which are involved in the regulation of constitutive and inducible expression of major histocompatibility complex class I (MHC I). It was speculated that<jats:named-content content-type=\"genus-species\">Chlamydia<\/jats:named-content>suppresses the surface presentation of MHC I in order to evade an effective immune response. Nevertheless, a recent study suggested that RFX5 and NF-\u03baB p65 may not serve as target substrates for CPAF-mediated degradation, raising concerns about the proposed MHC I subversion by<jats:named-content content-type=\"genus-species\">Chlamydia<\/jats:named-content>. Hence, we investigated the direct influence of<jats:named-content content-type=\"genus-species\">Chlamydia<\/jats:named-content>on MHC I expression and surface presentation in infected host cells. By using nine different human cells and cell lines infected with<jats:named-content content-type=\"genus-species\">C. trachomatis<\/jats:named-content>(serovar D or LGV2), we demonstrate that chlamydial infection does not interfere with expression, maturation, transport, and surface presentation of MHC I, suggesting functional antigen processing in bacterium-infected cells. Our findings provide novel insights into the interaction of chlamydiae with their host cells and should be taken into consideration for the design of future therapies and vaccines.<\/jats:p>","DOI":"10.1128\/iai.01473-13","type":"journal-article","created":{"date-parts":[[2013,12,17]],"date-time":"2013-12-17T03:16:28Z","timestamp":1387250188000},"page":"993-1006","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":9,"title":["Chlamydia trachomatis-Infected Epithelial Cells and Fibroblasts Retain the Ability To Express Surface-Presented Major Histocompatibility Complex Class I Molecules"],"prefix":"10.1128","volume":"82","author":[{"given":"Danny","family":"K\u00e4gebein","sequence":"first","affiliation":[{"name":"Institute of Immunology, Federal Research Institute of Animal Health, Friedrich Loeffler Institute, Greifswald, Isle of Riems, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Melanie","family":"Gutjahr","sequence":"additional","affiliation":[{"name":"Institute of Immunology, Federal Research Institute of Animal Health, Friedrich Loeffler Institute, Greifswald, Isle of Riems, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christina","family":"Gro\u00dfe","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology, Friedrich Schiller University of Jena, Jena, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Annette B.","family":"Vogel","sequence":"additional","affiliation":[{"name":"Institute of Immunology, Federal Research Institute of Animal Health, Friedrich Loeffler Institute, Greifswald, Isle of Riems, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J\u00fcrgen","family":"R\u00f6del","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology, Friedrich Schiller University of Jena, Jena, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael R.","family":"Knittler","sequence":"additional","affiliation":[{"name":"Institute of Immunology, Federal Research Institute of Animal Health, Friedrich Loeffler Institute, Greifswald, Isle of Riems, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpobgyn.2006.06.003"},{"key":"e_1_3_2_3_2","first-page":"41","article-title":"Chlamydia trachomatis as a cause of acute perihepatitis associated with pelvic inflammatory disease","volume":"57","author":"Dalaker K","year":"1981","unstructured":"DalakerKGjonnaessHKvileGUrnesAAnestadGBerganT. 1981. 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