{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T11:08:58Z","timestamp":1773659338172,"version":"3.50.1"},"reference-count":31,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2016,7,1]],"date-time":"2016-07-01T00:00:00Z","timestamp":1467331200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2016,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Peptidoglycan recognition proteins (PGLYRPs) are innate immune components that recognize the peptidoglycan and lipopolysaccharides of bacteria and exhibit antibacterial activity. Recently, the obligate intracellular parasite\n            <jats:named-content content-type=\"genus-species\">Chlamydia trachomatis<\/jats:named-content>\n            was shown to have peptidoglycan. However, the antichlamydial activity of PGLYRPs has not yet been demonstrated. The aim of our study was to test whether PGLYRPs exhibit antibacterial activity against\n            <jats:named-content content-type=\"genus-species\">C. trachomatis<\/jats:named-content>\n            . Thus, we cloned the regions containing the human\n            <jats:italic>Pglyrp1<\/jats:italic>\n            ,\n            <jats:italic>Pglyrp2<\/jats:italic>\n            ,\n            <jats:italic>Pglyrp3<\/jats:italic>\n            , and\n            <jats:italic>Pglyrp4<\/jats:italic>\n            genes for subsequent expression in human cell lines. We obtained stable HeLa cell lines that secrete recombinant human PGLYRPs into culture medium. We also generated purified recombinant PGLYRP1, -2, and -4 and confirmed their activities against Gram-positive (\n            <jats:named-content content-type=\"genus-species\">Bacillus subtilis<\/jats:named-content>\n            ) and Gram-negative (\n            <jats:named-content content-type=\"genus-species\">Escherichia coli<\/jats:named-content>\n            ) bacteria. Furthermore, we examined the activities of recombinant PGLYRPs against\n            <jats:named-content content-type=\"genus-species\">C. trachomatis<\/jats:named-content>\n            and determined their MICs. We also observed a decrease in the infectious ability of chlamydial elementary bodies in the next generation after a single exposure to PGLYRPs. Finally, we demonstrated that PGLYRPs attach to\n            <jats:named-content content-type=\"genus-species\">C. trachomatis<\/jats:named-content>\n            elementary bodies and activate the expression of the chlamydial two-component stress response system. Thus, PGLYRPs inhibit the development of chlamydial infection.\n          <\/jats:p>","DOI":"10.1128\/iai.01495-15","type":"journal-article","created":{"date-parts":[[2016,5,10]],"date-time":"2016-05-10T03:04:56Z","timestamp":1462849496000},"page":"2124-2130","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":12,"title":["Recombinant Human Peptidoglycan Recognition Proteins Reveal Antichlamydial Activity"],"prefix":"10.1128","volume":"84","author":[{"given":"Pavel","family":"Bobrovsky","sequence":"first","affiliation":[{"name":"Federal Research and Clinical Center of Physical-Chemical Medicine, Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Valentin","family":"Manuvera","sequence":"additional","affiliation":[{"name":"Federal Research and Clinical Center of Physical-Chemical Medicine, Moscow, Russia"},{"name":"Moscow Institute of Physics and Technology (State University), Dolgoprudny, Moscow Region, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nadezhda","family":"Polina","sequence":"additional","affiliation":[{"name":"Federal Research and Clinical Center of Physical-Chemical Medicine, Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Oleg","family":"Podgorny","sequence":"additional","affiliation":[{"name":"Federal Research and Clinical Center of Physical-Chemical Medicine, Moscow, Russia"},{"name":"Koltzov Institute of Developmental Biology of the Russian Academy of Sciences, Moscow, Russia"},{"name":"Moscow Institute of Physics and Technology (State University), Dolgoprudny, Moscow Region, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kirill","family":"Prusakov","sequence":"additional","affiliation":[{"name":"Federal Research and Clinical Center of Physical-Chemical Medicine, Moscow, Russia"},{"name":"Moscow Institute of Physics and Technology (State University), Dolgoprudny, Moscow Region, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vadim","family":"Govorun","sequence":"additional","affiliation":[{"name":"Federal Research and Clinical Center of Physical-Chemical Medicine, Moscow, Russia"},{"name":"Moscow Institute of Physics and Technology (State University), Dolgoprudny, Moscow Region, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vassili","family":"Lazarev","sequence":"additional","affiliation":[{"name":"Federal Research and Clinical Center of Physical-Chemical Medicine, Moscow, Russia"},{"name":"Moscow Institute of Physics and Technology (State University), Dolgoprudny, Moscow Region, 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