{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T02:08:19Z","timestamp":1790388499126,"version":"4.1.0"},"reference-count":24,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2009,7,1]],"date-time":"2009-07-01T00:00:00Z","timestamp":1246406400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2009,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:italic>Streptococcus pneumoniae<\/jats:italic>\n            sortase A (SrtA) is a transpeptidase that is highly conserved among pneumococcal strains, whose involvement in adhesion\/colonization has been reported. We found that intraperitoneal immunization with recombinant SrtA conferred to mice protection against\n            <jats:italic>S. pneumoniae<\/jats:italic>\n            intraperitoneal challenge and that the passive transfer of immune serum before intraperitoneal challenge was also protective. Moreover, by using the intranasal challenge model, we observed a significant reduction of bacteremia when mice were intraperitoneally immunized with SrtA, while a moderate decrease of lung infection was achieved by intranasal immunization, even though no influence on nasopharynx colonization was seen. Taken together, our results suggest that SrtA is a good candidate for inclusion in a multicomponent, protein-based, pneumococcal vaccine.\n          <\/jats:p>","DOI":"10.1128\/iai.01516-08","type":"journal-article","created":{"date-parts":[[2009,5,12]],"date-time":"2009-05-12T00:45:01Z","timestamp":1242089101000},"page":"2957-2961","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":22,"title":["Sortase A Confers Protection against\n            <i>Streptococcus pneumoniae<\/i>\n            in Mice"],"prefix":"10.1128","volume":"77","author":[{"given":"Claudia","family":"Gianfaldoni","sequence":"first","affiliation":[{"name":"Novartis Vaccines and Diagnostics s.r.l., Research Center, Siena, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia","family":"Maccari","sequence":"additional","affiliation":[{"name":"Novartis Vaccines and Diagnostics s.r.l., Research Center, Siena, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Pancotto","sequence":"additional","affiliation":[{"name":"Novartis Vaccines and Diagnostics s.r.l., Research Center, Siena, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giacomo","family":"Rossi","sequence":"additional","affiliation":[{"name":"University of Camerino, Department of Veterinary Sciences, Matelica, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Markus","family":"Hilleringmann","sequence":"additional","affiliation":[{"name":"Novartis Vaccines and Diagnostics s.r.l., Research Center, Siena, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Werner","family":"Pansegrau","sequence":"additional","affiliation":[{"name":"Novartis Vaccines and Diagnostics s.r.l., Research Center, Siena, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonia","family":"Sinisi","sequence":"additional","affiliation":[{"name":"Novartis Vaccines and Diagnostics s.r.l., Research Center, Siena, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monica","family":"Moschioni","sequence":"additional","affiliation":[{"name":"Novartis Vaccines and Diagnostics s.r.l., Research Center, Siena, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vega","family":"Masignani","sequence":"additional","affiliation":[{"name":"Novartis Vaccines and Diagnostics s.r.l., Research Center, Siena, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rino","family":"Rappuoli","sequence":"additional","affiliation":[{"name":"Novartis Vaccines and Diagnostics s.r.l., Research Center, Siena, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giuseppe","family":"Del Giudice","sequence":"additional","affiliation":[{"name":"Novartis Vaccines and Diagnostics s.r.l., Research Center, Siena, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paolo","family":"Ruggiero","sequence":"additional","affiliation":[{"name":"Novartis Vaccines and Diagnostics s.r.l., Research Center, Siena, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.1.75-80.2001"},{"key":"e_1_3_2_3_2","first-page":"459","year":"2004","unstructured":"Briles, D. E., S. K. Hollingshead, and J. Paton. 2004. Pneumococcal common proteins and other vaccine strategies, p. 459-469. In M. M. Levine, J. B. Kaper, R. Rappuoli, M. A. Liu, and M. F. Good (ed.), New generation vaccines, 3rd ed. Marcel Dekker, Inc., New York, NY.","journal-title":"New generation vaccines"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.femsle.2005.09.052"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01400-06"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.2217\/17460913.3.1.23"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1399-302X.2003.00076.x"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.5.2758-2765.2003"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.6.3342-3350.2005"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00379-08"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(06)68703-5"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1109869"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.70.1.192-221.2006"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.080520697"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01103-06"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2005.06.009"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1586\/14760584.2.2.285"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1586\/14760584.6.1.47"},{"key":"e_1_3_2_19_2","first-page":"421","year":"2004","unstructured":"Rappuoli, R., A. J. Pollard, and E. R. Moxon. 2004. Meningococcal coniugate and protein-based vaccines, p. 421-426. In M. M. Levine, J. B. Kaper, R. Rappuoli, M. A. Liu, and M. F. Good (ed.), New generation vaccines, 3rd ed. Marcel Dekker, Inc., New York, NY.","journal-title":"New generation vaccines"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.2471\/BLT.07.048769"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.297.16.1784"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.22.12424"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2007.08.032"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkh078"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2006.08.010"}],"container-title":["Infection and Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.01516-08","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.01516-08","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T08:03:53Z","timestamp":1646467433000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/IAI.01516-08"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,7]]},"references-count":24,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2009,7]]}},"alternative-id":["10.1128\/IAI.01516-08"],"URL":"https:\/\/doi.org\/10.1128\/iai.01516-08","relation":{},"ISSN":["0019-9567","1098-5522"],"issn-type":[{"value":"0019-9567","type":"print"},{"value":"1098-5522","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,7]]},"assertion":[{"value":"2008-12-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2009-04-29","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2009-07-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}