{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T05:00:31Z","timestamp":1790312431459,"version":"4.1.0"},"reference-count":45,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2014,9,1]],"date-time":"2014-09-01T00:00:00Z","timestamp":1409529600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2014,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Elongation factor P (EF-P) is a universally conserved bacterial translation factor. In many bacteria, EF-P is posttranslationally modified by PoxA, which covalently attaches a \u03b2-lysine to a conserved lysine residue of EF-P. Here we show that both EF-P and PoxA are necessary for virulence of the human diarrheal pathogen\n            <jats:named-content content-type=\"genus-species\">Shigella flexneri<\/jats:named-content>\n            . Loss of either EF-P or PoxA leads to an impaired ability of\n            <jats:named-content content-type=\"genus-species\">S. flexneri<\/jats:named-content>\n            to invade epithelial cells and form plaques in an epithelial cell monolayer. Proteomic analysis of\n            <jats:italic>efp<\/jats:italic>\n            and\n            <jats:italic>poxA<\/jats:italic>\n            deletion mutants revealed decreased levels of several virulence effector proteins, including IpaA, -B, and -C and IcsA. Additionally, mRNA levels of\n            <jats:italic>virB<\/jats:italic>\n            and\n            <jats:italic>virF<\/jats:italic>\n            , which encode master virulence regulators, were decreased in the\n            <jats:italic>efp<\/jats:italic>\n            mutant. The reduction in\n            <jats:italic>virF<\/jats:italic>\n            transcription was at least partially due to decreased levels of CpxA, which activates\n            <jats:italic>virF<\/jats:italic>\n            through the response regulator CpxR. The role of CpxAR in reduced synthesis of VirF and its downstream effectors was indicated by restoration of invasion when a mutation resulting in constitutively activated CpxR was introduced into the\n            <jats:italic>efp<\/jats:italic>\n            mutant. Thus, modified EF-P is required for appropriate synthesis of proteins involved in the virulence of this bacterial pathogen.\n          <\/jats:p>","DOI":"10.1128\/iai.01532-13","type":"journal-article","created":{"date-parts":[[2014,6,17]],"date-time":"2014-06-17T02:44:12Z","timestamp":1402973052000},"page":"3612-3621","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":29,"title":["Elongation Factor P and Modifying Enzyme PoxA Are Necessary for Virulence of Shigella flexneri"],"prefix":"10.1128","volume":"82","author":[{"given":"Hannah E.","family":"Marman","sequence":"first","affiliation":[{"name":"Department of Molecular Biosciences and Institute for Cellular and Molecular Biology, The University of Texas, Austin, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra R.","family":"Mey","sequence":"additional","affiliation":[{"name":"Department of Molecular Biosciences and Institute for Cellular and Molecular Biology, The University of Texas, Austin, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shelley M.","family":"Payne","sequence":"additional","affiliation":[{"name":"Department of Molecular Biosciences and Institute for Cellular and Molecular Biology, The University of Texas, Austin, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.femsre.2003.07.002"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1001519806727"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.86.10.3867"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1462-5822.2001.00143.x"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.49.1.164-171.1985"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.170.6.2480-2484.1988"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.3.1254"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1989.tb00210.x"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1988.tb00067.x"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.43.1.195-201.1984"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.177.17.5062-5069.1995"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.172.11.6274-6281.1990"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1992.tb01385.x"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.176.13.4187-4191.1994"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2001.02646.x"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.180.14.3522-3528.1998"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.175.19.6142-6149.1993"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00800-12"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkr521"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00589-10"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00112-07"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1229017"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1219536110"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00180-13"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1175800"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb.1889"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1186\/1745-6150-5-3"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio.1001"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2010.06.021"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.183.1.36-45.2001"},{"key":"e_1_3_3_32_2","volume-title":"Molecular cloning: a laboratory manual","author":"Sambrook J","year":"2001","unstructured":"SambrookJRussellDW. 2001. 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