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There is limited understanding of leptospiral pathogenesis; therefore, further elucidation of the mechanisms involved would aid in vaccine development and the prevention of infection. HtpG (\n            <jats:italic>h<\/jats:italic>\n            igh-\n            <jats:italic>t<\/jats:italic>\n            emperature\n            <jats:italic>p<\/jats:italic>\n            rotein\n            <jats:italic>G<\/jats:italic>\n            ) is the bacterial homolog to the highly conserved molecular chaperone Hsp90 and is important in the stress responses of many bacteria. The specific role of HtpG, especially in bacterial pathogenesis, remains largely unknown. Through the use of an\n            <jats:named-content content-type=\"genus-species\">L. interrogans<\/jats:named-content>\n            <jats:italic>htpG<\/jats:italic>\n            transposon insertion mutant, this study demonstrates that\n            <jats:named-content content-type=\"genus-species\">L. interrogans<\/jats:named-content>\n            HtpG is essential for virulence in the hamster model of acute leptospirosis. Complementation of the\n            <jats:italic>htpG<\/jats:italic>\n            mutant completely restored virulence. Surprisingly, the\n            <jats:italic>htpG<\/jats:italic>\n            mutant did not appear to show sensitivity to heat or oxidative stress, phenotypes common in\n            <jats:italic>htpG<\/jats:italic>\n            mutants in other bacterial species. Furthermore, the mutant did not show increased sensitivity to serum complement, reduced survival within macrophages, or altered protein or lipopolysaccharide expression. The underlying cause for attenuation thus remains unknown, but HtpG is a novel leptospiral virulence factor and one of only a very small number identified to date.\n          <\/jats:p>","DOI":"10.1128\/iai.01546-13","type":"journal-article","created":{"date-parts":[[2013,12,24]],"date-time":"2013-12-24T04:24:10Z","timestamp":1387859050000},"page":"1123-1131","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":49,"title":["High-Temperature Protein G Is an Essential Virulence Factor of Leptospira interrogans"],"prefix":"10.1128","volume":"82","author":[{"given":"Amy M.","family":"King","sequence":"first","affiliation":[{"name":"Australian Research Council Centre of Excellence in Structural and Functional Microbial Genomics, Department of Microbiology, Monash University, Victoria, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriela","family":"Pretre","sequence":"additional","affiliation":[{"name":"Institut Pasteur, Unit\u00e9 de Biologie des Spiroch\u00e8tes, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thanatchaporn","family":"Bartpho","sequence":"additional","affiliation":[{"name":"Melioidosis Research Center, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rasana W.","family":"Sermswan","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia","family":"Toma","sequence":"additional","affiliation":[{"name":"Department of Molecular Bacteriology and Immunology, Graduate School of Medicine, University of the Ryukyus, Nishihara, Okinawa, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toshihiko","family":"Suzuki","sequence":"additional","affiliation":[{"name":"Department of Molecular Bacteriology and Immunology, Graduate School of Medicine, University of the Ryukyus, Nishihara, Okinawa, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Azad","family":"Eshghi","sequence":"additional","affiliation":[{"name":"Institut Pasteur, Unit\u00e9 de Biologie des Spiroch\u00e8tes, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mathieu","family":"Picardeau","sequence":"additional","affiliation":[{"name":"Institut Pasteur, Unit\u00e9 de Biologie des Spiroch\u00e8tes, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ben","family":"Adler","sequence":"additional","affiliation":[{"name":"Australian Research Council Centre of Excellence in Structural and Functional Microbial Genomics, Department of Microbiology, Monash University, Victoria, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerald L.","family":"Murray","sequence":"additional","affiliation":[{"name":"Australian Research Council Centre of Excellence in Structural and Functional Microbial Genomics, Department of Microbiology, Monash University, Victoria, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2009.03.012"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(03)00830-2"},{"key":"e_1_3_2_4_2","volume-title":"Leptospira and leptospirosis","author":"Faine S","year":"1999","unstructured":"FaineSAdlerBBolinCPerolatP. 1999. 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