{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T12:16:00Z","timestamp":1790338560540,"version":"4.1.0"},"reference-count":52,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2008,5,1]],"date-time":"2008-05-01T00:00:00Z","timestamp":1209600000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2008,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Brucellosis is still a widespread zoonotic disease. Very little is known about the interaction between<jats:italic>Brucella abortus<\/jats:italic>and trophoblastic cells, which is essential for better understanding the pathogenesis of the<jats:italic>Brucella<\/jats:italic>-induced placentitis and abortion, a key event for transmission of the disease. The goal of this study was to evaluate the profile of gene expression by bovine trophoblastic cells during infection with<jats:italic>B. abortus<\/jats:italic>. Explants of chorioallantoic membranes were inoculated with<jats:italic>B. abortus<\/jats:italic>strain 2308. Microarray analysis was performed at 4 h after infection, and expression of cytokines and chemokines by trophoblastic cells was assessed by real-time reverse transcription-PCR at 6 and 12 h after inoculation. In addition, cytokine and chemokine expression in placentomes from experimentally infected cows was evaluated. Expression of proinflammatory genes by trophoblastic cells was suppressed at 4 h after inoculation, whereas a significant upregulation of CXC chemokines, namely, CXCL6 (GCP-2) and CXCL8 (interleukin 8), was observed at 12 but not at 6 h after inoculation. Placentomes of experimentally infected cows had a similar profile of chemokine expression, with upregulation of CXCL6 and CXCL8. Our data indicate that<jats:italic>B. abortus<\/jats:italic>modulates the innate immune response by trophoblastic cells, suppressing the expression of proinflammatory mediators during the early stages of infection that is followed by a delayed and mild expression of proinflammatory chemokines, which is similar to the profile of chemokine expression in the placentomes of experimentally infected cows. This trophoblastic response is likely to contribute to the pathogenesis of<jats:italic>B. abortus<\/jats:italic>-induced placentitis.<\/jats:p>","DOI":"10.1128\/iai.01554-07","type":"journal-article","created":{"date-parts":[[2008,3,4]],"date-time":"2008-03-04T01:35:04Z","timestamp":1204594504000},"page":"1897-1907","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":58,"title":["Modulation of the Bovine Trophoblastic Innate Immune Response by<i>Brucella abortus<\/i>"],"prefix":"10.1128","volume":"76","author":[{"given":"Alcina V.","family":"Carvalho Neta","sequence":"first","affiliation":[{"name":"Departamento de Cli\u0301nica e Cirurgia Veterina\u0301ria and Departamento de Medicina Veterina\u0301ria Preventiva, Escola de Veterina\u0301ria, Universidade Federal de Minas Gerais, 31270-901 Belo Horizonte, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana P. R.","family":"Stynen","sequence":"additional","affiliation":[{"name":"Departamento de Cli\u0301nica e Cirurgia Veterina\u0301ria and Departamento de Medicina Veterina\u0301ria Preventiva, Escola de Veterina\u0301ria, Universidade Federal de Minas Gerais, 31270-901 Belo Horizonte, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatiane A.","family":"Paixa\u0303o","sequence":"additional","affiliation":[{"name":"Departamento de Cli\u0301nica e Cirurgia Veterina\u0301ria and Departamento de Medicina Veterina\u0301ria Preventiva, Escola de Veterina\u0301ria, Universidade Federal de Minas Gerais, 31270-901 Belo Horizonte, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karina L.","family":"Miranda","sequence":"additional","affiliation":[{"name":"Departamento de Cli\u0301nica e Cirurgia Veterina\u0301ria and Departamento de Medicina Veterina\u0301ria Preventiva, Escola de Veterina\u0301ria, Universidade Federal de Minas Gerais, 31270-901 Belo Horizonte, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabiana L.","family":"Silva","sequence":"additional","affiliation":[{"name":"Departamento de Cli\u0301nica e Cirurgia Veterina\u0301ria and Departamento de Medicina Veterina\u0301ria Preventiva, Escola de Veterina\u0301ria, Universidade Federal de Minas Gerais, 31270-901 Belo Horizonte, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christelle M.","family":"Roux","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Immunology, University of California at Davis, Davis, California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rene\u0301e M.","family":"Tsolis","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Immunology, University of California at Davis, Davis, California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robin E.","family":"Everts","sequence":"additional","affiliation":[{"name":"Department of Animal Sciences, University of Illinois, Urbana, Illinois"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harris A.","family":"Lewin","sequence":"additional","affiliation":[{"name":"Department of Animal Sciences, University of Illinois, Urbana, Illinois"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L. Garry","family":"Adams","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pathobiology, Texas A&amp;M University, College Station, Texas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alex F.","family":"Carvalho","sequence":"additional","affiliation":[{"name":"Instituto Ludwig de Pesquisa sobre o Ca\u0302ncer, Sa\u0303o Paulo, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrey P.","family":"Lage","sequence":"additional","affiliation":[{"name":"Departamento de Cli\u0301nica e Cirurgia Veterina\u0301ria and Departamento de Medicina Veterina\u0301ria Preventiva, Escola de Veterina\u0301ria, Universidade Federal de Minas Gerais, 31270-901 Belo Horizonte, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renato L.","family":"Santos","sequence":"additional","affiliation":[{"name":"Departamento de Cli\u0301nica e Cirurgia Veterina\u0301ria and Departamento de Medicina Veterina\u0301ria Preventiva, Escola de Veterina\u0301ria, Universidade Federal de Minas Gerais, 31270-901 Belo Horizonte, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1177\/030098588802500104"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1177\/030098588602300301"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1177\/030098588602300302"},{"key":"e_1_3_2_5_2","first-page":"e631","volume":"7","year":"2007","unstructured":"Barqueiro-Calvo, E., E. Chaves-Olarte, D. S. Weiss, C. Guzm\u00e1n-Verri, C. Chacon-Diaz, A. Rucavado, I. Moriyon, and E. Moreno. 2007. Brucella abortus uses a stealthy strategy to avoid activation of the innate immune system during the onset of infection. PLoS ONE7:e631.","journal-title":"PLoS ONE"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0897.1997.tb00253.x"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)76559-4"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.1.176-186.2004"},{"key":"e_1_3_2_9_2","first-page":"131","year":"1990","unstructured":"Crawford, R. P., J. D. Huber, and B. S. Adams. 1990. Epidemiology and surveillance, pp. 131-151. In K. Nielsen and J. R. Duncan, Animal brucellosis. CRC Press, Boca Raton, FL.","journal-title":"Animal brucellosis."},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1177\/030098589002700503"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.58.7.2320-2328.1990"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/dxh148"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.61.11.4569-4574.1993"},{"key":"e_1_3_2_14_2","first-page":"424","volume":"45","year":"1984","unstructured":"Enright, F. M., J. V. Walker, G. Jeffers, and B. L. Deyoe. 1984. Cellular and humoral responses of Brucella abortus-infected bovine fetuses. Am. J. Vet. Res.45:424-430.","journal-title":"Am. J. Vet. Res."},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.3.1125-1133.2003"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1135(02)00212-2"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0264.1973.tb00253.x"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.173.7.4635"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.60.4.1385-1389.1992"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1177\/030098588602300317"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2567.2005.02143.x"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.171.3.1441"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.8.4823-4830.2001"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI117676"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.16.1.65-78.2003"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.86.14.5227"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2005.00629.x"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1152\/physiolgenomics.00188.2007"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1177\/030098588902600609"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80412-2"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01855-06"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.5.2387-2392.1998"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.12.5711-5724.1998"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2006.04.020"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01571-06"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2007.00922.x"},{"key":"e_1_3_2_38_2","doi-asserted-by":"crossref","first-page":"359","DOI":"10.2460\/ajvr.1992.53.3.359","volume":"53","year":"1992","unstructured":"Samartino, L. E., and F. M. Enright. 1992. Interaction of bovine chorioallantoic membrane explants with three strains of Brucella abortus. Am. J. Vet. Res.53:359-363.","journal-title":"Am. J. Vet. Res."},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/0147-9571(93)90001-L"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/0147-9571(95)00018-6"},{"key":"e_1_3_2_41_2","first-page":"34","volume":"6","year":"1998","unstructured":"Santos, R. L., M. T. D. Peixoto, R. Serakides, G. M. Costa, and N. E. Martins. 1998. Detecci\u00f3n de Brucella abortus (muestra B19) por el complejo inmunoenzim\u00e1tico avidina-biotina-peroxidasa en el test\u00edculo y en el epid\u00eddimo de bovinos inoculados experimentalmente. Arch. Reprod. Anim.6:34-41.","journal-title":"Arch. Reprod. Anim."},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1354\/vp.39-2-200"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1038\/193047a0"},{"key":"e_1_3_2_44_2","first-page":"631","volume":"42","year":"1961","unstructured":"Smith, H., J. Keppie, J. H. Pearce, R. Fuller, and A. E. Williams. 1961. The chemical basis of the virulence of Brucella abortus. I. Isolation of Brucella abortus from bovine foetal tissue. Br. J. Exp. Pathol.42:631.","journal-title":"Br. J. Exp. Pathol."},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.21.120601.141126"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1348-0421.2006.tb03810.x"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1186\/1477-7827-2-77"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1262\/jrd.16072"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1186\/1477-7827-5-17"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.8.5137-5143.2005"},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.1007\/s429-001-8004-7"},{"key":"e_1_3_2_52_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/21.2.283"},{"key":"e_1_3_2_53_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.64.7.2782-2786.1996"}],"container-title":["Infection and Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.01554-07","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.01554-07","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,2,23]],"date-time":"2024-02-23T03:20:50Z","timestamp":1708658450000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/IAI.01554-07"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,5]]},"references-count":52,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2008,5]]}},"alternative-id":["10.1128\/IAI.01554-07"],"URL":"https:\/\/doi.org\/10.1128\/iai.01554-07","relation":{},"ISSN":["0019-9567","1098-5522"],"issn-type":[{"value":"0019-9567","type":"print"},{"value":"1098-5522","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,5]]},"assertion":[{"value":"2007-11-23","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2008-01-30","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2008-05-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}