{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T02:21:35Z","timestamp":1790302895646,"version":"4.1.0"},"reference-count":65,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2008,5,1]],"date-time":"2008-05-01T00:00:00Z","timestamp":1209600000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2008,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            In gram-negative bacteria, iron acquisition proteins are commonly regulated by Fur (\n            <jats:italic>f<\/jats:italic>\n            erric\n            <jats:italic>u<\/jats:italic>\n            ptake\n            <jats:italic>r<\/jats:italic>\n            egulator), which binds iron-regulated promoters (the Fur box). We hypothesized that\n            <jats:italic>Coxiella burnetii<\/jats:italic>\n            requires iron and employs an iron-regulatory system and used various approaches to define a Fur regulon. Cloned\n            <jats:italic>C. burnetii fur<\/jats:italic>\n            complemented an\n            <jats:italic>Escherichia coli fur<\/jats:italic>\n            deletion mutant. A ferrous iron transporter gene (CBU1766), a putative iron binding protein-encoding gene (CBU0970), and a cation efflux pump gene (CBU1362) were identified by genome annotation and using a Fur titration assay. Bioinformatically predicted Fur box-containing promoters were tested for transcriptional control by iron. Five genes demonstrated at least a twofold induction with minimal iron. Putatively regulated genes were evaluated in a two-plasmid regulator\/promoter heterologous expression system. These data suggested a very limited Fur-regulated system in\n            <jats:italic>C. burnetii<\/jats:italic>\n            . In an in vitro tissue culture model, a significant increase in bacterial growth was observed with infected cells treated with deferoxamine in comparison to growth under iron-replete conditions. In an iron-overloaded animal model in vivo, the level of bacterial growth detected in the iron-injected animals was significantly decreased in comparison to growth in control animals. In a low-iron-diet animal model, a significant increase in splenomegaly was observed, but no significant change in bacterial growth was identified. The small number of predicted iron acquisition systems, few Fur-regulated genes, and enhanced replication under a decreased iron level predict a requirement of a low level of iron for survival, perhaps to avoid creation of additional reactive oxygen radicals.\n          <\/jats:p>","DOI":"10.1128\/iai.01609-07","type":"journal-article","created":{"date-parts":[[2008,3,4]],"date-time":"2008-03-04T01:35:04Z","timestamp":1204594504000},"page":"2189-2201","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":46,"title":["Limited Role for Iron Regulation in\n            <i>Coxiella burnetii<\/i>\n            Pathogenesis"],"prefix":"10.1128","volume":"76","author":[{"given":"Heather L.","family":"Briggs","sequence":"first","affiliation":[{"name":"TAMUS Health Science Center, Department of Microbial and Molecular Pathogenesis, College Station, Texas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicolein","family":"Pul","sequence":"additional","affiliation":[{"name":"TAMUS Health Science Center, Department of Microbial and Molecular Pathogenesis, College Station, Texas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rekha","family":"Seshadri","sequence":"additional","affiliation":[{"name":"The Institute of Genomic Research, Rockville, Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mary J.","family":"Wilson","sequence":"additional","affiliation":[{"name":"TAMUS Health Science Center, Department of Microbial and Molecular Pathogenesis, College Station, Texas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia","family":"Tersteeg","sequence":"additional","affiliation":[{"name":"TAMUS Health Science Center, Department of Microbial and Molecular Pathogenesis, College Station, Texas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kasi E.","family":"Russell-Lodrigue","sequence":"additional","affiliation":[{"name":"TAMUS Health Science Center, Department of Microbial and Molecular Pathogenesis, College Station, Texas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masako","family":"Andoh","sequence":"additional","affiliation":[{"name":"TAMUS Health Science Center, Department of Microbial and Molecular Pathogenesis, College Station, Texas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas J.","family":"Ba\u0308umler","sequence":"additional","affiliation":[{"name":"University of California, Department of Medical Microbiology and Immunology, Davis, California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James E.","family":"Samuel","sequence":"additional","affiliation":[{"name":"TAMUS Health Science Center, Department of Microbial and Molecular Pathogenesis, College Station, 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