{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,8]],"date-time":"2026-02-08T00:52:40Z","timestamp":1770511960756,"version":"3.49.0"},"reference-count":50,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2014,7,1]],"date-time":"2014-07-01T00:00:00Z","timestamp":1404172800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2014,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Cutaneous leishmaniasis is a sand fly-transmitted disease characterized by skin ulcers that carry significant scarring and social stigmatization. Over the past years, there has been cumulative evidence that immunity to specific sand fly salivary proteins confers a significant level of protection against leishmaniasis. In this study, we used an attenuated strain of\n            <jats:named-content content-type=\"genus-species\">Listeria monocytogenes<\/jats:named-content>\n            as a vaccine expression system for LJM11, a sand fly salivary protein identified as a good vaccine candidate. We observed that mice were best protected against an intradermal needle challenge with\n            <jats:named-content content-type=\"genus-species\">Leishmania major<\/jats:named-content>\n            and sand fly saliva when vaccinated intravenously. However, this protection was short-lived. Importantly, groups of vaccinated mice were protected long term when challenged with infected sand flies. Protection correlated with smaller lesion size, fewer scars, and better parasite control between 2 and 6 weeks postchallenge compared to the control group of mice vaccinated with the parent\n            <jats:named-content content-type=\"genus-species\">L. monocytogenes<\/jats:named-content>\n            strain not expressing LJM11. Moreover, protection correlated with high numbers of CD4\n            <jats:sup>+<\/jats:sup>\n            , gamma interferon-positive (IFN-\u03b3\n            <jats:sup>+<\/jats:sup>\n            ), tumor necrosis factor alpha-positive\/negative (TNF-\u03b1\n            <jats:sup>+\/\u2212<\/jats:sup>\n            ), interleukin-10-negative (IL-10\n            <jats:sup>\u2212<\/jats:sup>\n            ) cells and low numbers of CD4\n            <jats:sup>+<\/jats:sup>\n            IFN-\u03b3\n            <jats:sup>+\/\u2212<\/jats:sup>\n            TNF-\u03b1\n            <jats:sup>\u2212<\/jats:sup>\n            IL-10\n            <jats:sup>+<\/jats:sup>\n            T cells at 2 weeks postchallenge. Overall, our data indicate that delivery of LJM11 by\n            <jats:named-content content-type=\"genus-species\">Listeria<\/jats:named-content>\n            is a promising vaccination strategy against cutaneous leishmaniasis inducing long-term protection against ulcer formation following a natural challenge with infected sand flies.\n          <\/jats:p>","DOI":"10.1128\/iai.01633-14","type":"journal-article","created":{"date-parts":[[2014,4,15]],"date-time":"2014-04-15T03:38:32Z","timestamp":1397533112000},"page":"2736-2745","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":14,"title":["A Listeria monocytogenes-Based Vaccine That Secretes Sand Fly Salivary Protein LJM11 Confers Long-Term Protection against Vector-Transmitted Leishmania major"],"prefix":"10.1128","volume":"82","author":[{"given":"Delbert S.","family":"Abi Abdallah","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, Cornell University, Ithaca, New York, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alan","family":"Pavinski Bitar","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Cornell University, Ithaca, New York, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fabiano","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Vector Molecular Biology Section, Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, Maryland, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudio","family":"Meneses","sequence":"additional","affiliation":[{"name":"Vector Molecular Biology Section, Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, Maryland, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Justin J.","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Cornell University, Ithaca, New York, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susana","family":"Mendez","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Cornell University, Ithaca, New York, USA"},{"name":"Baker Institute for Animal Health, Cornell University, Ithaca, New York, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shaden","family":"Kamhawi","sequence":"additional","affiliation":[{"name":"Vector Molecular Biology Section, Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, Maryland, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jesus G.","family":"Valenzuela","sequence":"additional","affiliation":[{"name":"Vector Molecular Biology Section, Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, Maryland, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H\u00e9l\u00e8ne","family":"Marquis","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Cornell University, Ithaca, New York, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0035671"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2012.00110"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.188.10.1941"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.3344436"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3083.2007.01964.x"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.290.5495.1351"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0001139"},{"key":"e_1_3_3_9_2","first-page":"231","article-title":"The Bagdad boil deploys to the United States","volume":"90","author":"Aronson SM","year":"2007","unstructured":"AronsonSM. 2007. 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