{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T00:40:30Z","timestamp":1791852030023,"version":"4.3.4"},"reference-count":34,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2014,7,1]],"date-time":"2014-07-01T00:00:00Z","timestamp":1404172800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2014,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content content-type=\"genus-species\">Chlamydia trachomatis<\/jats:named-content>\n            is an obligate intracellular mucosotropic pathogen of significant medical importance. It is the etiological agent of blinding trachoma and bacterial sexually transmitted diseases, infections that afflict hundreds of millions of people globally. The\n            <jats:named-content content-type=\"genus-species\">C. trachomatis<\/jats:named-content>\n            polymorphic membrane protein D (PmpD) is a highly conserved autotransporter and the target of broadly cross-reactive neutralizing antibodies; however, its role in host-pathogen interactions is unknown. Here we employed a targeted reverse genetics approach to generate a\n            <jats:italic>pmpD<\/jats:italic>\n            null mutant that was used to define the role of PmpD in the pathogenesis of chlamydial infection. We show that\n            <jats:italic>pmpD<\/jats:italic>\n            is not an essential chlamydial gene and the\n            <jats:italic>pmpD<\/jats:italic>\n            null mutant has no detectable deficiency in cultured murine cells or in a murine mucosal infection model. Notably, however, the\n            <jats:italic>pmpD<\/jats:italic>\n            null mutant was significantly attenuated for macaque eyes and cultured human cells. A reduction in\n            <jats:italic>pmpD<\/jats:italic>\n            null infection of human endocervical cells was associated with a deficiency in chlamydial attachment to cells. Collectively, our results show that PmpD is a chlamydial virulence factor that functions in early host-cell interactions. This study is the first of its kind using reverse genetics to evaluate the contribution of a\n            <jats:named-content content-type=\"genus-species\">C. trachomatis<\/jats:named-content>\n            gene to disease pathogenesis.\n          <\/jats:p>","DOI":"10.1128\/iai.01686-14","type":"journal-article","created":{"date-parts":[[2014,4,15]],"date-time":"2014-04-15T03:38:32Z","timestamp":1397533112000},"page":"2756-2762","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":32,"title":["Chlamydia trachomatis Polymorphic Membrane Protein D Is a Virulence Factor Involved in Early Host-Cell Interactions"],"prefix":"10.1128","volume":"82","author":[{"given":"Laszlo","family":"Kari","sequence":"first","affiliation":[{"name":"Laboratory of Intracellular Parasites, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timothy R.","family":"Southern","sequence":"additional","affiliation":[{"name":"Laboratory of Intracellular Parasites, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carey J.","family":"Downey","sequence":"additional","affiliation":[{"name":"Laboratory of Intracellular Parasites, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heather S.","family":"Watkins","sequence":"additional","affiliation":[{"name":"Laboratory of Intracellular Parasites, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linnell B.","family":"Randall","sequence":"additional","affiliation":[{"name":"Laboratory of Intracellular Parasites, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lacey D.","family":"Taylor","sequence":"additional","affiliation":[{"name":"Laboratory of Intracellular Parasites, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gail L.","family":"Sturdevant","sequence":"additional","affiliation":[{"name":"Laboratory of Intracellular Parasites, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William M.","family":"Whitmire","sequence":"additional","affiliation":[{"name":"Laboratory of Intracellular Parasites, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harlan D.","family":"Caldwell","sequence":"additional","affiliation":[{"name":"Laboratory of Intracellular Parasites, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1136\/bjo.2008.148494"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM197803092981005"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM197802232980805"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM197803022980905"},{"key":"e_1_3_3_6_2","volume-title":"Global prevalence and incidence of selected curable sexually transmitted infections: overview and estimates","author":"World Health Organization","year":"2001","unstructured":"World Health Organization. 2001. 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