{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T10:00:23Z","timestamp":1760608823066},"reference-count":38,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2014,11,1]],"date-time":"2014-11-01T00:00:00Z","timestamp":1414800000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2014,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Our previous study demonstrated that treatment of<jats:named-content content-type=\"genus-species\">Coxiella burnetii<\/jats:named-content>with the phase I lipopolysaccharide (PI-LPS)-targeted monoclonal antibody (MAb) 1E4 significantly inhibited<jats:named-content content-type=\"genus-species\">C. burnetii<\/jats:named-content>infection in mice, suggesting that 1E4 is a protective MAb. To determine whether passive transfer of antibodies (Abs) can provide protection against<jats:named-content content-type=\"genus-species\">C. burnetii<\/jats:named-content>natural infection, we examined if passive transfer of 1E4 would protect SCID mice against<jats:named-content content-type=\"genus-species\">C. burnetii<\/jats:named-content>aerosol infection. The results indicated that 1E4 conferred significant protection against aerosolized<jats:named-content content-type=\"genus-species\">C. burnetii<\/jats:named-content>, suggesting that 1E4 may be useful for preventing<jats:named-content content-type=\"genus-species\">C. burnetii<\/jats:named-content>natural infection. To further understand the mechanisms of 1E4-mediated protection and to test the possibility of using humanized 1E4 to prevent<jats:named-content content-type=\"genus-species\">C. burnetii<\/jats:named-content>infection, we examined whether the Fab fragment of 1E4 (Fab1E4), a recombinant murine single-chain variable fragment (muscFv1E4), and a humanized single-chain variable fragment (huscFv1E4) retained the ability of 1E4 to inhibit<jats:named-content content-type=\"genus-species\">C. burnetii<\/jats:named-content>infection. The results indicated that Fab1E4, muscFv1E4, and huscFv1E4 were able to inhibit<jats:named-content content-type=\"genus-species\">C. burnetii<\/jats:named-content>infection in mice but that their ability to inhibit<jats:named-content content-type=\"genus-species\">C. burnetii<\/jats:named-content>infection was lower than that of 1E4. In addition, treatment of<jats:named-content content-type=\"genus-species\">C. burnetii<\/jats:named-content>with Fab1E4, muscFv1E4, or huscFv1E4 can block<jats:named-content content-type=\"genus-species\">C. burnetii<\/jats:named-content>infection of macrophages. Interestingly, treatment of<jats:named-content content-type=\"genus-species\">C. burnetii<\/jats:named-content>with huscFv1E4 can significantly reduce<jats:named-content content-type=\"genus-species\">C. burnetii<\/jats:named-content>infectivity in human macrophages. This report provides the first evidence to demonstrate that the humanized variable fragments of an LPS-specific MAb can neutralize<jats:named-content content-type=\"genus-species\">C. burnetii<\/jats:named-content>infection and appears to be a promising step toward the potential use of a humanized MAb as emergency prophylaxis against<jats:named-content content-type=\"genus-species\">C. burnetii<\/jats:named-content>exposure.<\/jats:p>","DOI":"10.1128\/iai.01695-14","type":"journal-article","created":{"date-parts":[[2014,8,12]],"date-time":"2014-08-12T04:05:46Z","timestamp":1407816346000},"page":"4530-4541","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":9,"title":["Characterization of a Lipopolysaccharide-Targeted Monoclonal Antibody and Its Variable Fragments as Candidates for Prophylaxis against the Obligate Intracellular Bacterial Pathogen Coxiella burnetii"],"prefix":"10.1128","volume":"82","author":[{"given":"Ying","family":"Peng","sequence":"first","affiliation":[{"name":"Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Missouri\u2014Columbia, Columbia, Missouri, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura","family":"Schoenlaub","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Missouri\u2014Columbia, Columbia, Missouri, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexandra","family":"Elliott","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Missouri\u2014Columbia, Columbia, Missouri, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William J.","family":"Mitchell","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Missouri\u2014Columbia, Columbia, Missouri, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guoquan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Missouri\u2014Columbia, Columbia, Missouri, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2009.07.016"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-94-007-4315-1_1"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.12.4.518"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/qjmed\/95.8.491"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1001\/archinte.159.2.167"},{"key":"e_1_3_2_7_2","first-page":"382","article-title":"Q fever in the Netherlands from 2007 to 2010","volume":"68","author":"Delsing CE","year":"2010","unstructured":"DelsingCEKullbergBJBleeker-RoversCP. 2010. 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