{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T05:35:57Z","timestamp":1790746557354,"version":"4.1.0"},"reference-count":63,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2014,8,1]],"date-time":"2014-08-01T00:00:00Z","timestamp":1406851200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2014,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content content-type=\"genus-species\">Burkholderia pseudomallei<\/jats:named-content>\n            , the etiologic agent of melioidosis, is an opportunistic pathogen that harbors a wide array of secretion systems, including a type II secretion system (T2SS), three type III secretion systems (T3SS), and six type VI secretion systems (T6SS). The proteins exported by these systems provide\n            <jats:named-content content-type=\"genus-species\">B. pseudomallei<\/jats:named-content>\n            with a growth advantage\n            <jats:italic>in vitro<\/jats:italic>\n            and\n            <jats:italic>in vivo<\/jats:italic>\n            , but relatively little is known about the full repertoire of exoproducts associated with each system. In this study, we constructed deletion mutations in\n            <jats:italic>gspD<\/jats:italic>\n            and\n            <jats:italic>gspE<\/jats:italic>\n            , T2SS genes encoding an outer membrane secretin and a cytoplasmic ATPase, respectively. The secretion profiles of\n            <jats:named-content content-type=\"genus-species\">B. pseudomallei<\/jats:named-content>\n            MSHR668 and its T2SS mutants were noticeably different when analyzed by SDS-PAGE. We utilized liquid chromatography-tandem mass spectrometry (LC-MS\/MS) to identify proteins present in the supernatants of\n            <jats:named-content content-type=\"genus-species\">B. pseudomallei<\/jats:named-content>\n            MSHR668 and\n            <jats:named-content content-type=\"genus-species\">B. pseudomallei<\/jats:named-content>\n            \u0394\n            <jats:italic>gspD<\/jats:italic>\n            grown in rich and minimal media. The MSHR668 supernatants contained 48 proteins that were either absent or substantially reduced in the supernatants of \u0394\n            <jats:italic>gspD<\/jats:italic>\n            strains. Many of these proteins were putative hydrolytic enzymes, including 12 proteases, two phospholipases, and a chitinase. Biochemical assays validated the LC-MS\/MS results and demonstrated that the export of protease, phospholipase C, and chitinase activities is T2SS dependent. Previous studies had failed to identify the mechanism of secretion of TssM, a deubiquitinase that plays an integral role in regulating the innate immune response. Here we present evidence that TssM harbors an atypical signal sequence and that its secretion is mediated by the T2SS. This study provides the first in-depth characterization of the\n            <jats:named-content content-type=\"genus-species\">B. pseudomallei<\/jats:named-content>\n            T2SS secretome.\n          <\/jats:p>","DOI":"10.1128\/iai.01739-14","type":"journal-article","created":{"date-parts":[[2014,5,28]],"date-time":"2014-05-28T06:43:50Z","timestamp":1401259430000},"page":"3214-3226","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":64,"title":["Proteomic Analysis of the Burkholderia pseudomallei Type II Secretome Reveals Hydrolytic Enzymes, Novel Proteins, and the Deubiquitinase TssM"],"prefix":"10.1128","volume":"82","author":[{"given":"Mary N.","family":"Burtnick","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, University of South Alabama, Mobile, Alabama, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul J.","family":"Brett","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of South Alabama, Mobile, Alabama, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"DeShazer","sequence":"additional","affiliation":[{"name":"Bacteriology Division, United States Army Medical Research Institute of Infectious Diseases, Frederick, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","first-page":"61083","article-title":"Possession, use, and transfer of select agents and toxins; biennial review","volume":"77","author":"CDC","year":"2012","unstructured":"CDC. 2012. 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