{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T13:36:17Z","timestamp":1780925777017,"version":"3.54.1"},"reference-count":88,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2006,6,1]],"date-time":"2006-06-01T00:00:00Z","timestamp":1149120000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2006,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p><jats:italic>Campylobacter jejuni<\/jats:italic>is a leading cause of bacterial food-borne diarrheal disease throughout the world and the most frequent antecedent of autoimmune neuropathy Guillain-Barre\u0301 syndrome. While infection is associated with immune memory, little is known regarding the role of the epithelium in targeting dendritic cells (DC) for initiating the appropriate adaptive immune response to<jats:italic>C. jejuni<\/jats:italic>. The objective of this study was to define the role for the intestinal epithelium in the induction of the adaptive immune response in<jats:italic>C. jejuni<\/jats:italic>infection by assessing the production of DC and T-cell chemoattractants. Human T84 epithelial cells were used as model intestinal epithelia. Infection of T84 cells with<jats:italic>C. jejuni<\/jats:italic>dose- and time-dependently up-regulated DC and T-cell chemokine gene transcription and secretion. Induction required live bacteria and was in the physiologically relevant direction for attraction of mucosal immunocytes.<jats:italic>C. jejuni<\/jats:italic>-activated NF-\u03baB signaling was shown to be essential for proinflammatory chemokine secretion. Notably,<jats:italic>C. jejuni<\/jats:italic>secretion occurred independently of flagellin identification by Toll-like receptor 5. Secretion of a DC chemoattractant by differing clinical<jats:italic>C. jejuni<\/jats:italic>isolates suggested adherence\/invasion were key virulence determinants of epithelial chemokine secretion. The regulated epithelial expression of DC and T-cell chemoattractants suggests a mechanism for the directed trafficking of immune cells required for the initiation of adaptive immunity in campylobacteriosis. Chemokine secretion occurs despite<jats:italic>Campylobacter<\/jats:italic>evasion of the flagellin pattern recognition receptor, suggesting that alternate host defense strategies limit disease pathogenesis.<\/jats:p>","DOI":"10.1128\/iai.01740-05","type":"journal-article","created":{"date-parts":[[2006,5,19]],"date-time":"2006-05-19T23:52:41Z","timestamp":1148082761000},"page":"3437-3447","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":51,"title":["Flagellin-Independent Regulation of Chemokine Host Defense in<i>Campylobacter jejuni<\/i>-Infected Intestinal Epithelium"],"prefix":"10.1128","volume":"74","author":[{"given":"Priscilla A.","family":"Johanesen","sequence":"first","affiliation":[{"name":"Department of Microbiology and Molecular Genetics, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, Wisconsin 53226"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael B.","family":"Dwinell","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Molecular Genetics, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, Wisconsin 53226"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1086\/319760"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid0501.990104"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0502040102"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.61.8.3466-3475.1993"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.61.6.2723-2726.1993"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.18.1.767"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/32588"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2004.01.028"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-695X.2000.tb01524.x"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/157.3.472"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1136\/gut.2004.062794"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(00)80201-0"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1984.246.3.C204"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.188.2.373"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.192.5.705"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1101\/gr.185801"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1053\/gast.2001.20914"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00112.2003"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.166.2.1248"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.61.11.4569-4574.1993"},{"key":"e_1_3_2_22_2","doi-asserted-by":"crossref","first-page":"1457","DOI":"10.4049\/jimmunol.163.3.1457","volume":"163","year":"1999","unstructured":"Elewaut, D., J. A. DiDonato, J. M. Kim, F. Truong, L. Eckmann, and M. F. Kagnoff. 1999. NF-kappa B is a central regulator of the intestinal epithelial cell innate immune response induced by infection with enteroinvasive bacteria. J. Immunol.163:1457-1466.","journal-title":"J. Immunol."},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1099\/00222615-37-5-319"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1038\/labinvest.3700176"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.54.2.283-287.1986"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/13.10.1255"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.167.4.1882"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.74.1.769-772.2006"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1038\/35074106"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2001.02376.x"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.1.88-93.1999"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.12.6535-6541.2000"},{"key":"e_1_3_2_33_2","doi-asserted-by":"crossref","first-page":"5550","DOI":"10.4049\/jimmunol.159.11.5550","volume":"159","year":"1997","unstructured":"Hobbie, S., L. M. Chen, R. J. Davis, and J. E. Galan. 1997. Involvement of mitogen-activated protein kinase pathways in the nuclear responses and cytokine production induced by Salmonella typhimurium in cultured intestinal epithelial cells. J. Immunol.159:5550-5559.","journal-title":"J. Immunol."},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.7.4437-4440.2005"},{"key":"e_1_3_2_35_2","first-page":"191","year":"2000","unstructured":"Hu, L., and D. J. Kopecko. 2000. Interactions of Campylobacter with eukaryotic cells: gut luminal colonization and mucosal invasion mechanisms, p. 191-217. In I. Nachamkin and M. J. Blaser (ed.), Campylobacter, 2nd ed. ASM Press, Washington, D.C.","journal-title":"Campylobacter"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11894-004-0050-1"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.191.8.1381"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.2001.280.4.G710"},{"key":"e_1_3_2_39_2","doi-asserted-by":"crossref","first-page":"226","DOI":"10.4049\/jimmunol.158.1.226","volume":"158","year":"1997","unstructured":"Jobin, C., S. Haskill, L. Mayer, A. Panja, and R. B. Sartor. 1997. Evidence for altered regulation of I kappa B alpha degradation in human colonic epithelial cells. J. Immunol.158:226-234.","journal-title":"J. Immunol."},{"key":"e_1_3_2_40_2","doi-asserted-by":"crossref","first-page":"410","DOI":"10.4049\/jimmunol.160.1.410","volume":"160","year":"1998","unstructured":"Jobin, C., A. Panja, C. Hellerbrand, Y. Iimuro, J. Didonato, D. A. Brenner, and R. B. Sartor. 1998. Inhibition of proinflammatory molecule production by adenovirus-mediated expression of a nuclear factor kappaB super-repressor in human intestinal epithelial cells. J. Immunol.160:410-418.","journal-title":"J. Immunol."},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.2000.278.3.C451"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI117676"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI119522"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.18.1.621"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.3.510-517.1999"},{"key":"e_1_3_2_46_2","first-page":"55","volume":"2","year":"2001","unstructured":"Konkel, M. E., M. R. Monteville, V. Rivera-Amill, and L. A. Joens. 2001. The pathogenesis of Campylobacter jejuni-mediated enteritis. Curr. Issues Intest. Microbiol.2:55-71.","journal-title":"Curr. Issues Intest. Microbiol."},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/152.3.592"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V96.7.2338"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M208241200"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.290.5490.354"},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.1038\/nri910"},{"key":"e_1_3_2_52_2","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_3_2_53_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2567.2005.02159.x"},{"key":"e_1_3_2_54_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-148-9-2753"},{"key":"e_1_3_2_55_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00565.2004"},{"key":"e_1_3_2_56_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-2952(00)00390-7"},{"key":"e_1_3_2_57_2","doi-asserted-by":"publisher","DOI":"10.1097\/00054725-200403000-00015"},{"key":"e_1_3_2_58_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.289.5484.1560"},{"key":"e_1_3_2_59_2","first-page":"57S","volume":"2001","year":"2001","unstructured":"Newell, D. G. 2001. Animal models of Campylobacter jejuni colonization and disease and the lessons to be learned from similar Helicobacter pylori models. Symp. Ser. Soc. Appl. Microbiol.2001:57S-67S.","journal-title":"Symp. Ser. Soc. Appl. Microbiol."},{"key":"e_1_3_2_60_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11894-001-0081-9"},{"key":"e_1_3_2_61_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.14.6884"},{"key":"e_1_3_2_62_2","doi-asserted-by":"publisher","DOI":"10.1038\/35001088"},{"key":"e_1_3_2_63_2","doi-asserted-by":"publisher","DOI":"10.3109\/03009749409103055"},{"key":"e_1_3_2_64_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2004.06.012"},{"key":"e_1_3_2_65_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.64.6.2070-2078.1996"},{"key":"e_1_3_2_66_2","doi-asserted-by":"publisher","DOI":"10.1078\/0171-2985-00094"},{"key":"e_1_3_2_67_2","doi-asserted-by":"publisher","DOI":"10.1038\/86373"},{"key":"e_1_3_2_68_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/168.1.210"},{"key":"e_1_3_2_69_2","doi-asserted-by":"publisher","DOI":"10.1038\/nri1499"},{"key":"e_1_3_2_70_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.2001.281.4.G890"},{"key":"e_1_3_2_71_2","first-page":"303","year":"2000","unstructured":"Scott, D. A., and T., D. R. 2000. Protection against Campylobacter infection and vaccine development, p. 303-319. In I. Nachamkin and M. J. Blaser (ed.), Campylobacter, 2nd ed. ASM Press, Washington, D.C.","journal-title":"Campylobacter"},{"key":"e_1_3_2_72_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.241308598"},{"key":"e_1_3_2_73_2","first-page":"69","year":"2000","unstructured":"Skirrow, M. B., and M. J. Blaser. 2000. Clinical aspects of Campylobacter infection, p. 69-88. In I. Nachamkin and M. J. Blaser (ed.), Campylobacter. ASM Press, Washington, D.C.","journal-title":"Campylobacter"},{"key":"e_1_3_2_74_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00208.2004"},{"key":"e_1_3_2_75_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2004.04175.x"},{"key":"e_1_3_2_76_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI8892"},{"key":"e_1_3_2_77_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.168.11.5621"},{"key":"e_1_3_2_78_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-4-33"},{"key":"e_1_3_2_79_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1521-4141(199902)29:02<633::AID-IMMU633>3.0.CO;2-I"},{"key":"e_1_3_2_80_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.crvi.2004.02.010"},{"key":"e_1_3_2_81_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1286-4579(99)80520-6"},{"key":"e_1_3_2_82_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.66.1.1-9.2000"},{"key":"e_1_3_2_83_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2004.00498.x"},{"key":"e_1_3_2_84_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.5.1934-1940.1998"},{"key":"e_1_3_2_85_2","doi-asserted-by":"crossref","first-page":"1737","DOI":"10.4049\/jimmunol.163.4.1737","volume":"163","year":"1999","unstructured":"Yang, D., O. M. Howard, Q. Chen, and J. J. Oppenheim. 1999. Cutting edge: immature dendritic cells generated from monocytes in the presence of TGF-beta 1 express functional C-C chemokine receptor 6. J. Immunol.163:1737-1741.","journal-title":"J. Immunol."},{"key":"e_1_3_2_86_2","doi-asserted-by":"publisher","DOI":"10.1053\/gast.1997.v113.pm9322516"},{"key":"e_1_3_2_87_2","first-page":"287","year":"2000","unstructured":"Young, V. B., D. B. Schauer, and J. G. Fox. 2000. Animal models of Campylobacter infection, p. 287-301. In I. Nachamkin nd M. J. Blaser (ed.), Campylobacter, 2nd ed. ASM Press, Washington, D.C.","journal-title":"Campylobacter"},{"key":"e_1_3_2_88_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0402391101"},{"key":"e_1_3_2_89_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.4.2120-2129.2003"}],"container-title":["Infection and Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.01740-05","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.01740-05","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,5,7]],"date-time":"2023-05-07T13:16:48Z","timestamp":1683465408000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/IAI.01740-05"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,6]]},"references-count":88,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2006,6]]}},"alternative-id":["10.1128\/IAI.01740-05"],"URL":"https:\/\/doi.org\/10.1128\/iai.01740-05","relation":{},"ISSN":["0019-9567","1098-5522"],"issn-type":[{"value":"0019-9567","type":"print"},{"value":"1098-5522","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,6]]},"assertion":[{"value":"2005-10-27","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2006-03-22","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2006-06-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}