{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T18:29:30Z","timestamp":1760984970181},"reference-count":81,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2014,6,1]],"date-time":"2014-06-01T00:00:00Z","timestamp":1401580800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2014,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Colonization of the human nasopharynx by\n            <jats:named-content content-type=\"genus-species\">Moraxella catarrhalis<\/jats:named-content>\n            is presumed to involve attachment of this bacterium to the mucosa. DNA microarray analysis was used to determine whether attachment of\n            <jats:named-content content-type=\"genus-species\">M. catarrhalis<\/jats:named-content>\n            to human bronchial epithelial (HBE) cells\n            <jats:italic>in vitro<\/jats:italic>\n            affected gene expression in this bacterium. Attachment affected expression of at least 454 different genes, with 163 being upregulated and 291 being downregulated. Among the upregulated genes was one (ORF113) previously annotated as encoding a protein with some similarity to outer membrane protein A (OmpA). The protein encoded by ORF113 was predicted to have a signal peptidase II cleavage site, and globomycin inhibition experiments confirmed that this protein was indeed a lipoprotein. The ORF113 protein also contained a predicted peptidoglycan-binding domain in its C-terminal half. The use of mutant and recombinant\n            <jats:named-content content-type=\"genus-species\">M. catarrhalis<\/jats:named-content>\n            strains confirmed that the ORF113 protein was present in outer membrane preparations, and this protein was also shown to be at least partially exposed on the bacterial cell surface. A mutant unable to produce the ORF113 protein showed little or no change in its growth rate\n            <jats:italic>in vitro<\/jats:italic>\n            , in its ability to attach to HBE cells\n            <jats:italic>in vitro<\/jats:italic>\n            , or in its autoagglutination characteristics, but it did exhibit a reduced ability to survive in the chinchilla nasopharynx. This is the first report of a lipoprotein essential to the ability of\n            <jats:named-content content-type=\"genus-species\">M. catarrhalis<\/jats:named-content>\n            to persist in an animal model.\n          <\/jats:p>","DOI":"10.1128\/iai.01745-14","type":"journal-article","created":{"date-parts":[[2014,3,19]],"date-time":"2014-03-19T04:09:28Z","timestamp":1395202168000},"page":"2287-2299","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":8,"title":["Identification of an Outer Membrane Lipoprotein Involved in Nasopharyngeal Colonization by Moraxella catarrhalis in an Animal Model"],"prefix":"10.1128","volume":"82","author":[{"given":"Wei","family":"Wang","sequence":"first","affiliation":[{"name":"Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephanie N.","family":"Joslin","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, Texas, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christine","family":"Pybus","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, Texas, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amanda S.","family":"Evans","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, University of Texas Southwestern Medical Center, Dallas, Texas, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Flora","family":"Lichaa","sequence":"additional","affiliation":[{"name":"Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chad A.","family":"Brautigam","sequence":"additional","affiliation":[{"name":"Department of Biophysics, University of Texas Southwestern Medical Center, Dallas, Texas, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eric J.","family":"Hansen","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, Texas, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"2529","volume-title":"Mandell, Douglas, and Bennett's principles and practice of infectious disease","author":"Murphy TF","year":"2005","unstructured":"MurphyTF. 2005. 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