{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T23:53:27Z","timestamp":1790380407026,"version":"4.1.0"},"reference-count":41,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2014,8,1]],"date-time":"2014-08-01T00:00:00Z","timestamp":1406851200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2014,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The native plasmid of both\n            <jats:named-content content-type=\"genus-species\">Chlamydia muridarum<\/jats:named-content>\n            and\n            <jats:named-content content-type=\"genus-species\">Chlamydia trachomatis<\/jats:named-content>\n            has been shown to control virulence and infectivity in mice and in lower primates. We recently described the development of a plasmid-based genetic transformation protocol for\n            <jats:named-content content-type=\"genus-species\">Chlamydia trachomatis<\/jats:named-content>\n            that for the first time provides a platform for the molecular dissection of the function of the chlamydial plasmid and its individual genes or coding sequences (CDS). In the present study, we transformed a plasmid-free lymphogranuloma venereum isolate of\n            <jats:named-content content-type=\"genus-species\">C. trachomatis<\/jats:named-content>\n            , serovar L2, with either the original shuttle vector (pGFP::SW2) or a derivative of pGFP::SW2 carrying a deletion of the plasmid CDS5 gene (pCDS5KO). Female mice were inoculated with these strains either intravaginally or transcervically. We found that transformation of the plasmid-free isolate with the intact pGFP::SW2 vector significantly enhanced infectivity and induction of host inflammatory responses compared to the plasmid-free parental isolate. Transformation with pCDS5KO resulted in infection courses and inflammatory responses not significantly different from those observed in mice infected with the plasmid-free isolate. These results indicate a critical role of plasmid CDS5 in\n            <jats:italic>in vivo<\/jats:italic>\n            fitness and in induction of inflammatory responses. To our knowledge, these are the first\n            <jats:italic>in vivo<\/jats:italic>\n            observations ascribing infectivity and virulence to a specific plasmid gene.\n          <\/jats:p>","DOI":"10.1128\/iai.01795-14","type":"journal-article","created":{"date-parts":[[2014,5,28]],"date-time":"2014-05-28T06:43:50Z","timestamp":1401259430000},"page":"3341-3349","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":31,"title":["Plasmid CDS5 Influences Infectivity and Virulence in a Mouse Model of Chlamydia trachomatis Urogenital Infection"],"prefix":"10.1128","volume":"82","author":[{"given":"K. H.","family":"Ramsey","sequence":"first","affiliation":[{"name":"Department of Microbiology &amp; Immunology, Chicago College of Osteopathic Medicine, Illinois Midwestern University, Downers Grove, Illinois, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. H.","family":"Schripsema","sequence":"additional","affiliation":[{"name":"Department of Microbiology &amp; Immunology, Chicago College of Osteopathic Medicine, Illinois Midwestern University, Downers Grove, Illinois, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B. J.","family":"Smith","sequence":"additional","affiliation":[{"name":"Department of Microbiology &amp; Immunology, Chicago College of Osteopathic Medicine, Illinois Midwestern University, Downers Grove, Illinois, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Clinical and Experimental Sciences, Molecular Microbiology Group, Faculty of Medicine, University of Southampton, Southampton, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B. 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