{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T23:02:16Z","timestamp":1791414136368,"version":"4.3.3"},"reference-count":49,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2014,6,1]],"date-time":"2014-06-01T00:00:00Z","timestamp":1401580800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2014,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Limited research has been conducted on the role of transcriptional regulators in relation to virulence in<jats:named-content content-type=\"genus-species\">Leptospira interrogans<\/jats:named-content>, the etiological agent of leptospirosis. Here, we identify an<jats:named-content content-type=\"genus-species\">L. interrogans<\/jats:named-content>locus that encodes a sensor protein, an anti-sigma factor antagonist, and two genes encoding proteins of unknown function. Transposon insertion into the gene encoding the sensor protein led to dampened transcription of the other 3 genes in this locus. This lb139 insertion mutant (the lb139<jats:sup>\u2212<\/jats:sup>mutant) displayed attenuated virulence in the hamster model of infection and reduced motility<jats:italic>in vitro<\/jats:italic>. Whole-transcriptome analyses using RNA sequencing revealed the downregulation of 115 genes and the upregulation of 28 genes, with an overrepresentation of gene products functioning in motility and signal transduction and numerous gene products with unknown functions, predicted to be localized to the extracellular space. Another significant finding encompassed suppressed expression of the majority of the genes previously demonstrated to be upregulated at physiological osmolarity, including the sphingomyelinase C precursor Sph2 and LigB. We provide insight into a possible requirement for transcriptional regulation as it relates to leptospiral virulence and suggest various biological processes that are affected due to the loss of native expression of this genetic locus.<\/jats:p>","DOI":"10.1128\/iai.01803-14","type":"journal-article","created":{"date-parts":[[2014,4,1]],"date-time":"2014-04-01T04:23:04Z","timestamp":1396326184000},"page":"2542-2552","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":40,"title":["A Putative Regulatory Genetic Locus Modulates Virulence in the Pathogen Leptospira interrogans"],"prefix":"10.1128","volume":"82","author":[{"given":"Azad","family":"Eshghi","sequence":"first","affiliation":[{"name":"Institut Pasteur, Biology of Spirochetes Unit, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00e9r\u00f4me","family":"Becam","sequence":"additional","affiliation":[{"name":"Institut Pasteur de Nouvelle-Cal\u00e9donie, Association Pasteur International Network, Noum\u00e9a, New Caledonia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ambroise","family":"Lambert","sequence":"additional","affiliation":[{"name":"Institut Pasteur, Biology of Spirochetes Unit, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Odile","family":"Sismeiro","sequence":"additional","affiliation":[{"name":"Plate-Forme Transcriptome et Epigenome, Departement G\u00e9nomes et G\u00e9n\u00e9tique, Institut Pasteur, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie-Agn\u00e8s","family":"Dillies","sequence":"additional","affiliation":[{"name":"Plate-Forme Transcriptome et Epigenome, Departement G\u00e9nomes et G\u00e9n\u00e9tique, Institut Pasteur, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernd","family":"Jagla","sequence":"additional","affiliation":[{"name":"Plate-Forme Transcriptome et Epigenome, Departement G\u00e9nomes et G\u00e9n\u00e9tique, Institut Pasteur, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"Jr.","given":"Elsio A.","family":"Wunder","sequence":"additional","affiliation":[{"name":"Yale School of Public Health, Department of Epidemiology of Microbial Disease, New Haven, Connecticut, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Albert I.","family":"Ko","sequence":"additional","affiliation":[{"name":"Yale School of Public Health, Department of Epidemiology of Microbial Disease, New Haven, Connecticut, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Yves","family":"Coppee","sequence":"additional","affiliation":[{"name":"Plate-Forme Transcriptome et Epigenome, Departement G\u00e9nomes et G\u00e9n\u00e9tique, Institut Pasteur, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cyrille","family":"Goarant","sequence":"additional","affiliation":[{"name":"Institut Pasteur de Nouvelle-Cal\u00e9donie, Association Pasteur International Network, Noum\u00e9a, New Caledonia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mathieu","family":"Picardeau","sequence":"additional","affiliation":[{"name":"Institut Pasteur, Biology of Spirochetes Unit, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2208"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2334-12-20"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00755-06"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-6-51"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00435-10"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-10-31"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0000857"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01619-06"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00911-12"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.70.9.5349-5356.2004"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.10.6106-6111.2004"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.182.18.5036-5045.2000"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2007.05813.x"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.4.1560-1568.1999"},{"key":"e_1_3_3_16_2","first-page":"45","article-title":"Nutrition of Leptospira pomona and growth of 13 other serotypes: fractionation of oleic albumin complex and a medium of bovine albumin and polysorbate 80","volume":"26","author":"Ellinghausen HC","year":"1965","unstructured":"EllinghausenHCJrMcCulloughWG. 1965. 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