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As pneumococci do not produce catalase or an inducible regulator of hydrogen peroxide, the mechanism of resistance to hydrogen peroxide is unclear. A gene responsible for resistance to hydrogen peroxide and iron in other streptococci is that encoding nonheme iron-containing ferritin,<jats:italic>dpr<\/jats:italic>, but previous attempts to study this gene in pneumococcus by generating a<jats:italic>dpr<\/jats:italic>mutant were unsuccessful. In the current study, we found that<jats:italic>dpr<\/jats:italic>is in an operon with the downstream genes<jats:italic>dhfr<\/jats:italic>and<jats:italic>clpX<\/jats:italic>. We generated a<jats:italic>dpr<\/jats:italic>deletion mutant which displayed normal early-log-phase and mid-log-phase growth in bacteriologic medium but survived less well at stationary phase; the addition of catalase partially rescued the growth defect. We showed that the<jats:italic>dpr<\/jats:italic>mutant is significantly more sensitive to pH, heat, iron concentration, and oxidative stress due to hydrogen peroxide. Using a mouse model of colonization, we also showed that the<jats:italic>dpr<\/jats:italic>mutant displays a reduced ability to colonize and is more rapidly cleared from the nasopharynx. Our results thus suggest that Dpr is important for pneumococcal resistance to stress and for nasopharyngeal colonization.<\/jats:p>","DOI":"10.1128\/iai.01829-14","type":"journal-article","created":{"date-parts":[[2014,7,8]],"date-time":"2014-07-08T02:41:30Z","timestamp":1404787290000},"page":"3939-3947","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":21,"title":["Effect of Nonheme Iron-Containing Ferritin Dpr in the Stress Response and Virulence of Pneumococci"],"prefix":"10.1128","volume":"82","author":[{"given":"Chun-Zhen","family":"Hua","sequence":"first","affiliation":[{"name":"Division of Infectious Diseases, Boston Children's Hospital, Harvard Medical School, Boston, Massachusetts, USA"},{"name":"Division of Infectious Disease, Children's Hospital, Zhejiang University School of Medicine, Hangzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Angela","family":"Howard","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Boston Children's Hospital, Harvard Medical School, Boston, Massachusetts, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard","family":"Malley","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Boston Children's Hospital, Harvard Medical School, Boston, Massachusetts, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying-Jie","family":"Lu","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Boston Children's Hospital, Harvard Medical School, Boston, Massachusetts, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.185.23.6815-6825.2003"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1061217"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.2834821"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.61.12.5401-5405.1993"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2001.02414.x"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1039\/c3mt20244e"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.11.6702-6706.2001"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.8.4389-4398.2002"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.6.12b.2646"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.6.3259"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.182.13.3740-3747.2000"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1139\/w01-027"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00477-08"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2010.10.058"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2004.03.009"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.184.11.2931-2939.2002"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2005.04756.x"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.23.8123-8136.2004"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0043445"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(85)90258-1"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.185.3.1010-1017.2003"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1999.tb13460.x"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng.2625"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.76.9.4350"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.4.2286-2293.2000"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1086\/323084"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000159"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.8.4870-4873.2001"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002989"},{"key":"e_1_3_2_31_2","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1016\/S1016-8478(23)13181-5","article-title":"Identification of essential genes in Streptococcus pneumoniae by allelic replacement mutagenesis","volume":"19","author":"Song JH","year":"2005","unstructured":"SongJHKoKSLeeJYBaekJYOhWSYoonHSJeongJYChunJ. 2005. 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