{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,5]],"date-time":"2025-11-05T20:39:26Z","timestamp":1762375166277},"reference-count":47,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2007,5,1]],"date-time":"2007-05-01T00:00:00Z","timestamp":1177977600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2007,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>The<jats:italic>NRAMP1<\/jats:italic>gene encodes a divalent cation transporter, located in the phagolysosomal membrane of macrophages, that has been associated with resistance to intracellular pathogens. In cattle, natural resistance against brucellosis has been associated with polymorphisms at the 3\u2032 untranslated region (3\u2032UTR) of the<jats:italic>NRAMP1<\/jats:italic>gene, which are detectable by single-strand conformational analysis (SSCA). This study aimed to evaluate the association between<jats:italic>NRAMP1<\/jats:italic>3\u2032UTR polymorphisms and resistance against bovine brucellosis in experimental and natural infections. In experimentally infected pregnant cows, abortion occurred in 42.1% of cows with a resistant genotype (SSCA<jats:sup>r<\/jats:sup>;<jats:italic>n<\/jats:italic>= 19) and in 43.1% of those with a susceptible genotype (SSCA<jats:sup>s<\/jats:sup>;<jats:italic>n<\/jats:italic>= 23). Furthermore, no association between intensity of pathological changes and genotype was detected. In a farm with a very high prevalence of bovine brucellosis, the percentages of strains of the SSCA<jats:sup>r<\/jats:sup>genotype were 86 and 84% in serologically positive (<jats:italic>n<\/jats:italic>= 64) and negative (<jats:italic>n<\/jats:italic>= 36) cows, respectively. Therefore, no association was found between the<jats:italic>NRAMP1<\/jats:italic>-resistant allele and the resistant phenotype in either experimental or naturally occurring brucellosis. To further support these results, bacterial intracellular survival was assessed in bovine monocyte-derived macrophages from cattle with either the resistant or susceptible genotype. In agreement with our previous results, no difference was observed in the rates of intracellular survival of<jats:italic>B. abortus<\/jats:italic>within macrophages from cattle with susceptible or resistant genotypes. Taken together, these results indicate that these polymorphisms at the<jats:italic>NRAMP1<\/jats:italic>3\u2032UTR do not affect resistance against<jats:italic>B. abortus<\/jats:italic>in cattle and that they are therefore not suitable markers of natural resistance against bovine brucellosis.<\/jats:p>","DOI":"10.1128\/iai.01855-06","type":"journal-article","created":{"date-parts":[[2007,3,13]],"date-time":"2007-03-13T05:34:26Z","timestamp":1173764066000},"page":"2493-2499","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":31,"title":["<i>NRAMP1<\/i>3\u2032 Untranslated Region Polymorphisms Are Not Associated with Natural Resistance to<i>Brucella abortus<\/i>in Cattle"],"prefix":"10.1128","volume":"75","author":[{"given":"Tatiane A.","family":"Paixa\u0303o","sequence":"first","affiliation":[{"name":"Departamento de Cli\u0301nica e Cirurgia Veterina\u0301ria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fernando P.","family":"Poester","sequence":"additional","affiliation":[{"name":"Departamento de Medicina Veterina\u0301ria Preventiva, Escola de Veterina\u0301ria, Universidade Federal de Minas Gerais, 31270-901 Belo Horizonte, Minas Gerais, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alcina V.","family":"Carvalho Neta","sequence":"additional","affiliation":[{"name":"Departamento de Cli\u0301nica e Cirurgia Veterina\u0301ria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A\u0301lan M.","family":"Borges","sequence":"additional","affiliation":[{"name":"Departamento de Cli\u0301nica e Cirurgia Veterina\u0301ria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrey P.","family":"Lage","sequence":"additional","affiliation":[{"name":"Departamento de Medicina Veterina\u0301ria Preventiva, Escola de Veterina\u0301ria, Universidade Federal de Minas Gerais, 31270-901 Belo Horizonte, Minas Gerais, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Renato L.","family":"Santos","sequence":"additional","affiliation":[{"name":"Departamento de Cli\u0301nica e Cirurgia Veterina\u0301ria"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.64.1081"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-2427(96)05652-8"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.20506\/rst.17.1.1085"},{"key":"e_1_3_2_5_2","unstructured":"Techniques for the brucellosis laboratory. 1988"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1135(02)00222-5"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.5.3110-3119.2001"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.2460\/ajvr.2000.61.1140"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.74.4.2115-2120.2006"},{"key":"e_1_3_2_10_2","first-page":"7","volume":"37","year":"1979","unstructured":"Bradley, D. 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