{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T20:53:00Z","timestamp":1791060780909,"version":"4.1.0"},"reference-count":33,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2007,6,1]],"date-time":"2007-06-01T00:00:00Z","timestamp":1180656000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2007,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The\n            <jats:italic>Brucella abortus<\/jats:italic>\n            type IV secretion system (T4SS), encoded by the\n            <jats:italic>virB<\/jats:italic>\n            genes, is essential for survival in mononuclear phagocytes in vitro. In the mouse model, a\n            <jats:italic>B. abortus virB<\/jats:italic>\n            mutant was initially able to colonize the spleen at the level of the wild type for approximately 3 to 5 days, which coincided with the development of adaptive immunity. To investigate the relationship between survival in macrophages cultivated in vitro and persistence in tissues in vivo, we tested the ability of mutant mice lacking components of adaptive immunity to eliminate the\n            <jats:italic>virB<\/jats:italic>\n            mutant from the spleen during a mixed infection with the\n            <jats:italic>B. abortus<\/jats:italic>\n            wild type.\n            <jats:italic>Ifng<\/jats:italic>\n            <jats:sup>\u2212\/\u2212<\/jats:sup>\n            or \u03b2\n            <jats:sub>\n              <jats:italic>2<\/jats:italic>\n            <\/jats:sub>\n            <jats:italic>m<\/jats:italic>\n            <jats:sup>\u2212\/\u2212<\/jats:sup>\n            mice were able to clear the\n            <jats:italic>virB<\/jats:italic>\n            mutant to the same degree as control mice. However, spleens of\n            <jats:italic>Rag1<\/jats:italic>\n            <jats:sup>\u2212\/\u2212<\/jats:sup>\n            mice and\n            <jats:italic>Igh6<\/jats:italic>\n            <jats:sup>\u2212\/\u2212<\/jats:sup>\n            mice were more highly colonized by the\n            <jats:italic>virB<\/jats:italic>\n            mutant than control mice after 14 to 21 days, suggesting that, in these mice, there is not an absolute requirement for the T4SS to mediate persistence of\n            <jats:italic>B. abortus<\/jats:italic>\n            in the spleen. Macrophages isolated from\n            <jats:italic>Igh6<\/jats:italic>\n            <jats:sup>\u2212\/\u2212<\/jats:sup>\n            mice killed the\n            <jats:italic>virB<\/jats:italic>\n            mutant to the same extent as macrophages from control mice, showing that the reduced ability of these mice to clear the\n            <jats:italic>virB<\/jats:italic>\n            mutant from the spleen does not correlate with diminished macrophage function in vitro. These results show that in the murine model host, the T4SS is required for persistence beyond 3 to 5 days after infection and suggest that the T4SS may contribute to evasion of adaptive immune mechanisms by\n            <jats:italic>B. abortus<\/jats:italic>\n            .\n          <\/jats:p>","DOI":"10.1128\/iai.01896-06","type":"journal-article","created":{"date-parts":[[2007,4,10]],"date-time":"2007-04-10T00:34:44Z","timestamp":1176165284000},"page":"2965-2973","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":60,"title":["Mice Lacking Components of Adaptive Immunity Show Increased\n            <i>Brucella abortus virB<\/i>\n            Mutant Colonization"],"prefix":"10.1128","volume":"75","author":[{"given":"Hortensia Garci\u0301a","family":"Rola\u0301n","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology and Immunology, University of California, One Shields Avenue, Davis, California 95616"},{"name":"Texas A&amp;M University System Health Science Center, Department of Medical Microbiology and Immunology, College Station, Texas 77843-1114"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rene\u0301e M.","family":"Tsolis","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Immunology, University of California, One Shields Avenue, Davis, California 95616"},{"name":"Texas A&amp;M University System Health Science Center, Department of Medical Microbiology and Immunology, College Station, Texas 77843-1114"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","unstructured":"Laboratory techniques in brucellosis 1975"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.6.3702-3713.2005"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20030088"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0406873102"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1462-5822.2001.00102.x"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.8456300"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1462-5822.2001.00131.x"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.9.5143-5149.2004"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-695X.1996.tb00060.x"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.3.1297-1303.2000"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.7.4102-4107.2000"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1286-4579(02)00076-X"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.9.5314-5320.2000"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.6.3020-3027.2003"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/350423a0"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.232454299"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.4.1730"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2000.02150.x"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.12.7053-7060.2003"},{"key":"e_1_3_2_21_2","first-page":"2147","volume":"47","year":"1986","unstructured":"Meador, V. 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