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Several different borrelial outer surface proteins have been identified as being able to bind host factor H, a regulator of the alternative pathway, leading to a hypothesis that such binding is important for borrelial resistance to complement. To test this hypothesis, the development of<jats:italic>B. burgdorferi<\/jats:italic>infection was compared between factor H-deficient and wild-type mice. Factor B- and C3-deficient mice were also studied to determine the relative roles of the alternative and classical\/lectin pathways in<jats:italic>B. burgdorferi<\/jats:italic>survival during mammalian infection. While it was predicted that<jats:italic>B. burgdorferi<\/jats:italic>should be impaired in its ability to infect factor H-deficient animals, quantitative analyses of bacterial loads indicated that those mice were infected at levels similar to those of wild-type and factor B- and C3-deficient mice. Ticks fed on infected factor H-deficient or wild-type mice all acquired similar numbers of bacteria. Indirect immunofluorescence analysis of<jats:italic>B. burgdorferi<\/jats:italic>acquired by feeding ticks from the blood of infected mice indicated that none of the bacteria had detectable levels of factor H on their outer surfaces, even though such bacteria express high levels of surface proteins capable of binding factor H. These findings demonstrate that the acquisition of host factor H is not essential for mammalian infection by<jats:italic>B. burgdorferi<\/jats:italic>and indicate that additional mechanisms are employed by the Lyme disease spirochete to evade complement-mediated killing.<\/jats:p>","DOI":"10.1128\/iai.01923-06","type":"journal-article","created":{"date-parts":[[2007,4,10]],"date-time":"2007-04-10T00:34:44Z","timestamp":1176165284000},"page":"3131-3139","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":33,"title":["<i>Borrelia burgdorferi<\/i>Binding of Host Complement Regulator Factor H Is Not Required for Efficient Mammalian Infection"],"prefix":"10.1128","volume":"75","author":[{"given":"Michael E.","family":"Woodman","sequence":"first","affiliation":[{"name":"Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky College of Medicine, Lexington, Kentucky 40536"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anne E.","family":"Cooley","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky College of Medicine, Lexington, Kentucky 40536"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jennifer C.","family":"Miller","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky College of Medicine, Lexington, Kentucky 40536"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John J.","family":"Lazarus","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Immunology, University of Toledo Health Sciences Campus, Toledo, Ohio 43614"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kathryn","family":"Tucker","sequence":"additional","affiliation":[{"name":"Statistics Collaborative, Inc., Washington, DC 20036"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomasz","family":"Bykowski","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky College of Medicine, Lexington, Kentucky 40536"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marina","family":"Botto","sequence":"additional","affiliation":[{"name":"Molecular Genetics and Rheumatology Section, Division of Medicine, Imperial College, Hammersmith Campus, London W12 0NN, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jens","family":"Hellwage","sequence":"additional","affiliation":[{"name":"Leibniz Institute for Natural Products Research and Infection Biology, Hans Kno\u0308ll Institute, Beutenbergstrasse 11a, D-07745 Jena, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. 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