{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T04:59:01Z","timestamp":1792126741043,"version":"4.4.0"},"reference-count":38,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2006,7,1]],"date-time":"2006-07-01T00:00:00Z","timestamp":1151712000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2006,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:italic>znuA<\/jats:italic>\n            is known to be an important factor for survival and normal growth under low Zn\n            <jats:sup>2+<\/jats:sup>\n            concentrations for\n            <jats:italic>Escherichia coli<\/jats:italic>\n            ,\n            <jats:italic>Haemophilus<\/jats:italic>\n            spp.,\n            <jats:italic>Neisseria gonorrhoeae<\/jats:italic>\n            , and\n            <jats:italic>Pasteurella multocida<\/jats:italic>\n            . We hypothesized that the\n            <jats:italic>znuA<\/jats:italic>\n            gene present in\n            <jats:italic>Brucella melitensis<\/jats:italic>\n            16 M would be similar to\n            <jats:italic>znuA<\/jats:italic>\n            in\n            <jats:italic>B. abortus<\/jats:italic>\n            and questioned whether it may also be an important factor for growth and virulence of\n            <jats:italic>Brucella abortus<\/jats:italic>\n            . Using the\n            <jats:italic>B. melitensis<\/jats:italic>\n            16 M genome sequence, primers were designed to construct a\n            <jats:italic>B. abortus<\/jats:italic>\n            deletion mutant. A\n            <jats:italic>znuA<\/jats:italic>\n            knockout mutation in\n            <jats:italic>B. abortus<\/jats:italic>\n            2308 (\n            <jats:italic>\u0394znuA<\/jats:italic>\n            ) was constructed and found to be lethal in low-Zn\n            <jats:sup>2+<\/jats:sup>\n            medium. When used to infect macrophages,\n            <jats:italic>\u0394znuA B. abortus<\/jats:italic>\n            showed minimal growth. Further study with\n            <jats:italic>\u0394znuA B. abortus<\/jats:italic>\n            showed that its virulence in BALB\/c mice was attenuated, and most of the bacteria were cleared from the spleen within 8 weeks. Protection studies confirmed the\n            <jats:italic>\u0394znuA<\/jats:italic>\n            mutant as a potential live vaccine, since protection against wild-type\n            <jats:italic>B. abortus<\/jats:italic>\n            2308 challenge was as effective as that obtained with the RB51 or S19 vaccine strain.\n          <\/jats:p>","DOI":"10.1128\/iai.01957-05","type":"journal-article","created":{"date-parts":[[2006,6,21]],"date-time":"2006-06-21T23:10:29Z","timestamp":1150931429000},"page":"3874-3879","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":81,"title":["Deletion of\n            <i>znuA<\/i>\n            Virulence Factor Attenuates\n            <i>Brucella abortus<\/i>\n            and Confers Protection against Wild-Type Challenge"],"prefix":"10.1128","volume":"74","author":[{"given":"Xinghong","family":"Yang","sequence":"first","affiliation":[{"name":"Veterinary Molecular Biology, Montana State University, Bozeman, Montana 59717-3610"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Todd","family":"Becker","sequence":"additional","affiliation":[{"name":"Veterinary Molecular Biology, Montana State University, Bozeman, Montana 59717-3610"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nancy","family":"Walters","sequence":"additional","affiliation":[{"name":"Veterinary Molecular Biology, Montana State University, Bozeman, Montana 59717-3610"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David W.","family":"Pascual","sequence":"additional","affiliation":[{"name":"Veterinary Molecular Biology, Montana State University, Bozeman, Montana 59717-3610"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.8.4911-4917.2004"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.10.6225-6230.2001"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2004.02.041"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2003.09.008"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2000.tb09019.x"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.7.4528-4535.2001"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-147-12-3201"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2001.tb10781.x"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0923-2508(01)01195-0"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.221575398"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.9.5143-5149.2004"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0307137101"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/mmbr.61.2.136-169.1997"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.1.547-550.2001"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1097(03)00131-9"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.5.2873-2880.2005"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4615-4863-8_50"},{"key":"e_1_3_2_19_2","first-page":"1092","volume":"49","year":"1988","unstructured":"Harmon, B. 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