{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T06:44:47Z","timestamp":1791614687080,"version":"4.3.4"},"reference-count":26,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2007,7,1]],"date-time":"2007-07-01T00:00:00Z","timestamp":1183248000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2007,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            FimH is the tip adhesin of mannose-specific type 1 fimbriae of\n            <jats:italic>Escherichia coli<\/jats:italic>\n            , which are critical to the pathogenesis of urinary tract infections. Point FimH mutations increasing monomannose (1M)-specific uroepithelial adhesion are commonly found in uropathogenic strains of\n            <jats:italic>E. coli<\/jats:italic>\n            . Here, we demonstrate the emergence of a mixed population of clonally identical\n            <jats:italic>E. coli<\/jats:italic>\n            strains in the urine of a patient with acute cystitis, where half of the isolates carried a glycine-to-arginine substitution at position 66 of the mature FimH. The R66 mutation induced an unusually strong 1M-binding phenotype and a 20-fold advantage in mouse bladder colonization. However,\n            <jats:italic>E. coli<\/jats:italic>\n            strains carrying FimH-R66, but not the parental FimH-G66, had disappeared from the patient's rectal and urine samples collected from 29 to 44 days later, demonstrating within-host instability of the R66 mutation. No FimH variants with R66 were identified in a large (&gt;600 strains) sequence database of\n            <jats:italic>fimH<\/jats:italic>\n            -positive\n            <jats:italic>E. coli<\/jats:italic>\n            strains. However, several strains carrying genes encoding FimH with either S66 or C66 mutations appeared to be relatively stable in the\n            <jats:italic>E. coli<\/jats:italic>\n            population. Relative to FimH-R66, the FimH-S66 and FimH-C66 variants mediated only moderate increases in 1M binding but preserved the ability to enhance binding under flow-induced shear conditions. In contrast, FimH-R66 completely lost shear-enhanced binding properties, with bacterial adhesion being inhibited by shear forces and lacking a rolling mode of binding. These functional trade-offs may determine the natural populational instability of this mutation or other pathoadaptive FimH mutations that confer dramatic increases in 1M binding strength.\n          <\/jats:p>","DOI":"10.1128\/iai.01963-06","type":"journal-article","created":{"date-parts":[[2007,5,15]],"date-time":"2007-05-15T00:58:35Z","timestamp":1179190715000},"page":"3548-3555","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":66,"title":["Differential Stability and Trade-Off Effects of Pathoadaptive Mutations in the\n            <i>Escherichia coli<\/i>\n            FimH Adhesin"],"prefix":"10.1128","volume":"75","author":[{"given":"Scott J.","family":"Weissman","sequence":"first","affiliation":[{"name":"Division of Infectious Disease, Children's Hospital and Regional Medical Center; Department of Pediatrics, University of Washington School of Medicine, Seattle, Washington 98195"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Viktoriya","family":"Beskhlebnaya","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Russian University of Peoples\u2019 Friendship, Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Veronika","family":"Chesnokova","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Washington School of Medicine, Seattle, Washington 98195"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sujay","family":"Chattopadhyay","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Washington School of Medicine, Seattle, Washington 98195"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Walter E.","family":"Stamm","sequence":"additional","affiliation":[{"name":"Division of Allergy and Infectious Disease, Department of Medicine, University of Washington School of Medicine, Seattle, Washington 98195"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas M.","family":"Hooton","sequence":"additional","affiliation":[{"name":"University of Miami Miller School of Medicine, Miami, Florida 33136"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evgeni V.","family":"Sokurenko","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Washington School of Medicine, Seattle, Washington 98195"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00899-06"},{"key":"e_1_3_2_3_2","first-page":"190","year":"1995","unstructured":"Arbeit, R. D. 1995. Laboratory procedures for the epidemiologic analysis of microorganisms, p. 190-208. In P. R. Murray, E. J. Baron, M. A. Pfaller, F. C. Tenover, and R. H. Yolken (ed.), Manual of clinical microbiology, 6th ed. American Society of Microbiology, Washington, DC.","journal-title":"Manual of clinical microbiology"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2006.05352.x"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.6.3619-3622.2003"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1086\/339343"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1067\/mlc.2002.121550"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.59.4.1567-1568.1991"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.282.21.2035"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1993.tb00911.x"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.282.5393.1494"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.72.4.3005-3010.2006"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.157.2.690-693.1984"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.3.1470-1480.2003"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.28.17880"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.15.8922"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.177.13.3680-3686.1995"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.176.3.748-755.1994"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msh136"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1446"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1529\/biophysj.105.066548"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2004.04226.x"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(02)00796-1"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2005.04985.x"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.252529799"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2006.05172.x"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.114.22.4095"}],"updated-by":[{"DOI":"10.1128\/iai.01228-07","type":"correction","label":"Correction","source":"publisher","updated":{"date-parts":[[2009,4,1]],"date-time":"2009-04-01T00:00:00Z","timestamp":1238544000000}}],"container-title":["Infection and Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.01963-06","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.01963-06","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T07:15:42Z","timestamp":1646464542000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/IAI.01963-06"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,7]]},"references-count":26,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2007,7]]}},"alternative-id":["10.1128\/IAI.01963-06"],"URL":"https:\/\/doi.org\/10.1128\/iai.01963-06","relation":{"correction":[{"id-type":"doi","id":"10.1128\/IAI.01228-07","asserted-by":"object"}]},"ISSN":["0019-9567","1098-5522"],"issn-type":[{"value":"0019-9567","type":"print"},{"value":"1098-5522","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,7]]},"assertion":[{"value":"2006-12-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2007-05-02","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2007-07-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}