{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T07:11:26Z","timestamp":1790233886815,"version":"4.1.0"},"reference-count":45,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2007,5,1]],"date-time":"2007-05-01T00:00:00Z","timestamp":1177977600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2007,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:italic>Bartonella quintana<\/jats:italic>\n            is a fastidious, gram-negative, rod-shaped bacterium that causes prolonged bacteremia in immunocompetent humans and severe infections in immunocompromised individuals. We sought to define the outer membrane subproteome of\n            <jats:italic>B. quintana<\/jats:italic>\n            in order to obtain insight into the biology and pathogenesis of this emerging pathogen and to identify the predominant\n            <jats:italic>B. quintana<\/jats:italic>\n            antigens targeted by the human immune system during infection. We isolated the total membrane proteins of\n            <jats:italic>B. quintana<\/jats:italic>\n            and identified 60 proteins by two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis and peptide mass fingerprinting. Using the newly constructed proteome map, we then utilized two-dimensional immunoblotting with sera from 21\n            <jats:italic>B. quintana<\/jats:italic>\n            -infected patients to identify 24 consistently recognized, immunoreactive\n            <jats:italic>B. quintana<\/jats:italic>\n            antigens that have potential relevance for pathogenesis and diagnosis. Among the outer membrane proteins, the\n            <jats:italic>v<\/jats:italic>\n            ariably expressed\n            <jats:italic>o<\/jats:italic>\n            uter\n            <jats:italic>m<\/jats:italic>\n            embrane\n            <jats:italic>p<\/jats:italic>\n            rotein adhesins (VompA and VompB), peptidyl-prolyl\n            <jats:italic>cis-trans<\/jats:italic>\n            -isomerase (PpI), and\n            <jats:italic>h<\/jats:italic>\n            emin-\n            <jats:italic>b<\/jats:italic>\n            inding\n            <jats:italic>p<\/jats:italic>\n            rotein\n            <jats:italic>E<\/jats:italic>\n            (HbpE) were recognized most frequently by sera from patients, which is consistent with surface expression of these virulence factors during human infection.\n          <\/jats:p>","DOI":"10.1128\/iai.01974-06","type":"journal-article","created":{"date-parts":[[2007,2,17]],"date-time":"2007-02-17T01:58:30Z","timestamp":1171677510000},"page":"2548-2561","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":49,"title":["Proteomic and Immunoblot Analyses of\n            <i>Bartonella quintana<\/i>\n            Total Membrane Proteins Identify Antigens Recognized by Sera from Infected Patients"],"prefix":"10.1128","volume":"75","author":[{"given":"Jenni K.","family":"Boonjakuakul","sequence":"first","affiliation":[{"name":"Division of Infectious Diseases, Department of Medicine, University of California at San Francisco, San Francisco, California 94143-0654"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helen L.","family":"Gerns","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Medicine, University of California at San Francisco, San Francisco, California 94143-0654"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu-Ting","family":"Chen","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Medicine, University of California at San Francisco, San Francisco, California 94143-0654"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linda D.","family":"Hicks","sequence":"additional","affiliation":[{"name":"Division of Biological Sciences, University of Montana, Missoula, Montana 59812"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael F.","family":"Minnick","sequence":"additional","affiliation":[{"name":"Division of Biological Sciences, University of Montana, Missoula, Montana 59812"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Scott E.","family":"Dixon","sequence":"additional","affiliation":[{"name":"Biomolecular Resource Center Mass Spectrometry Facility, University of California at San Francisco, San Francisco, California 94143"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven C.","family":"Hall","sequence":"additional","affiliation":[{"name":"Biomolecular Resource Center Mass Spectrometry Facility, University of California at San Francisco, San Francisco, California 94143"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jane E.","family":"Koehler","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Medicine, University of California at San Francisco, San Francisco, California 94143-0654"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0305659101"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200401019"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.4.949-955.2004"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkh121"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00245-06"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200401342"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.19.11478"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.6.3097-3105.2004"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2005.03.004"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1001\/archinte.1995.00430150164017"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkj149"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1086\/342065"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/CDLI.6.4.558-566.1999"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bti057"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.8.4818-4822.2003"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/1615-9861(200203)2:3<313::AID-PROT313>3.0.CO;2-7"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200204144"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1348-0421.2003.tb03419.x"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/CDLI.5.6.766-772.1998"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1988.39.173"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199712253372603"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1086\/375586"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(82)90515-0"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.6.1899-1905.1999"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.34.9.2270-2274.1996"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.45616-0"},{"key":"e_1_3_2_28_2","unstructured":"Molecular cloning: a laboratory manual 1982"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.35.9.2283-2287.1997"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.2.814-821.2003"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.12.6933-6942.2003"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1135(96)00010-7"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/147.5.838"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.109.1.89-95.1972"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.1150180309"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)41496-8"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.179.14.4545-4552.1997"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M209238200"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1086\/520454"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.1150150183"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(92)92032-B"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.femsle.2005.03.004"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200400933"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.61.12.4962-4971.1993"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00663-06"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0405284101"}],"container-title":["Infection and Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.01974-06","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.01974-06","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T07:05:27Z","timestamp":1646463927000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/IAI.01974-06"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,5]]},"references-count":45,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2007,5]]}},"alternative-id":["10.1128\/IAI.01974-06"],"URL":"https:\/\/doi.org\/10.1128\/iai.01974-06","relation":{},"ISSN":["0019-9567","1098-5522"],"issn-type":[{"value":"0019-9567","type":"print"},{"value":"1098-5522","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,5]]},"assertion":[{"value":"2006-12-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2007-02-10","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2007-05-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}