{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T02:20:24Z","timestamp":1767925224092,"version":"3.49.0"},"reference-count":54,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2014,10,1]],"date-time":"2014-10-01T00:00:00Z","timestamp":1412121600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2014,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>The Lyme disease spirochete,<jats:named-content content-type=\"genus-species\">Borrelia burgdorferi<\/jats:named-content>, occupies both a tick vector and mammalian host in nature. Considering the unique enzootic life cycle of<jats:named-content content-type=\"genus-species\">B. burgdorferi<\/jats:named-content>, it is not surprising that a large proportion of its genome is composed of hypothetical proteins not found in other bacterial pathogens.<jats:italic>bb0238<\/jats:italic>encodes a conserved hypothetical protein of unknown function that is predicted to contain a tetratricopeptide repeat (TPR) domain, a structural motif responsible for mediating protein-protein interactions. To evaluate the role of<jats:italic>bb0238<\/jats:italic>during mammalian infection, a<jats:italic>bb0238<\/jats:italic>-deficient mutant was constructed. The<jats:italic>bb0238<\/jats:italic>mutant was attenuated in mice infected via needle inoculation, and complementation of<jats:italic>bb0238<\/jats:italic>expression restored infectivity to wild-type levels.<jats:italic>bb0238<\/jats:italic>expression does not change in response to varying culture conditions, and thus, it appears to be constitutively expressed under<jats:italic>in vitro<\/jats:italic>conditions.<jats:italic>bb0238<\/jats:italic>is expressed in murine tissues during infection, though there was no significant change in expression levels among different tissue types. Localization studies indicate that BB0238 is associated with the inner membrane of the spirochete and is therefore unlikely to promote interaction with host ligands during infection.<jats:named-content content-type=\"genus-species\">B. burgdorferi<\/jats:named-content>clones containing point mutations in conserved residues of the putative TPR motif of BB0238 demonstrated attenuation in mice that was comparable to that in the<jats:italic>bb0238<\/jats:italic>deletion mutant, suggesting that BB0238 may contain a functional TPR domain.<\/jats:p>","DOI":"10.1128\/iai.01977-14","type":"journal-article","created":{"date-parts":[[2014,7,29]],"date-time":"2014-07-29T04:03:27Z","timestamp":1406606607000},"page":"4292-4306","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":23,"title":["BB0238, a Presumed Tetratricopeptide Repeat-Containing Protein, Is Required during Borrelia burgdorferi Mammalian Infection"],"prefix":"10.1128","volume":"82","author":[{"given":"Ashley M.","family":"Groshong","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, University of Arkansas for Medical Sciences, Little Rock, Arkansas, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Danielle E.","family":"Fortune","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Arkansas for Medical Sciences, Little Rock, Arkansas, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brendan P.","family":"Moore","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Arkansas for Medical Sciences, Little Rock, Arkansas, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Horace J.","family":"Spencer","sequence":"additional","affiliation":[{"name":"Department of Biostatistics, University of Arkansas for Medical Sciences, Little Rock, Arkansas, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert A.","family":"Skinner","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery, University of Arkansas for Medical Sciences, Little Rock, Arkansas, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William T.","family":"Bellamy","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Arkansas for Medical Sciences, Little Rock, Arkansas, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jon S.","family":"Blevins","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Arkansas for Medical Sciences, Little Rock, Arkansas, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.7043737"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2714"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198303313081301"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(11)60103-7"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI200421681"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/37551"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0502565102"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1086\/605846"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2013.06.001"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1086\/651172"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2009.06853.x"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0019536"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1521-1878(199911)21:11<932::AID-BIES5>3.0.CO;2-N"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.30.3.698-703.1992"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.31.5.1251-1255.1993"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-8-82"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00253-12"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02454-06"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.1701"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01118-07"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-11-17"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.11.6008-6018.1999"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-12-60"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.8.4864-4867.2004"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2010.02020.x"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.74.1.296-304.2006"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.170.12.5789-5796.1988"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.187.1.175-184.2005"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.12048"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2007.05746.x"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-4658.2005.04945.x"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1992.47.605"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.9.5190-5197.2000"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.9.5264-5269.2001"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.170.4.1427"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.164.12.6096"},{"key":"e_1_3_3_38_2","first-page":"811","article-title":"Lyme borreliosis in the severe combined immunodeficiency (scid) mouse manifests predominantly in the joints, heart, and liver","volume":"137","author":"Schaible UE","year":"1990","unstructured":"SchaibleUE GayS MuseteanuC KramerMD ZimmerG EichmannK MuseteanuU SimonMM. 1990. Lyme borreliosis in the severe combined immunodeficiency (scid) mouse manifests predominantly in the joints, heart, and liver. Am. J. Pathol. 137:811\u2013820.","journal-title":"Am. J. Pathol."},{"key":"e_1_3_3_39_2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.4049\/jimmunol.157.1.1","article-title":"Protective antibodies in murine Lyme disease arise independently of CD40 ligand","volume":"157","author":"Fikrig E","year":"1996","unstructured":"FikrigE BartholdSW ChenM GrewalIS CraftJ FlavellRA. 1996. Protective antibodies in murine Lyme disease arise independently of CD40 ligand. J. Immunol. 157:1\u20133.","journal-title":"J. Immunol."},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00514-12"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00450-09"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2009.06740.x"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-143-5-1681"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-141-1-85"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.032667699"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.6.3371-3383.2003"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.4.1689-1705.2003"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2000.02104.x"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00252-12"},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkp912"},{"key":"e_1_3_3_51_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibs.2003.10.007"},{"key":"e_1_3_3_52_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0959-440X(03)00105-2"},{"key":"e_1_3_3_53_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkl217"},{"key":"e_1_3_3_54_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.05014-11"},{"key":"e_1_3_3_55_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2003.03452.x"}],"container-title":["Infection and Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.01977-14","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.01977-14","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,15]],"date-time":"2023-07-15T15:16:12Z","timestamp":1689434172000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/IAI.01977-14"}},"subtitle":[],"editor":[{"given":"A.","family":"Camilli","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2014,10]]},"references-count":54,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2014,10]]}},"alternative-id":["10.1128\/IAI.01977-14"],"URL":"https:\/\/doi.org\/10.1128\/iai.01977-14","relation":{},"ISSN":["0019-9567","1098-5522"],"issn-type":[{"value":"0019-9567","type":"print"},{"value":"1098-5522","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,10]]},"assertion":[{"value":"2014-04-25","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-07-23","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-09-10","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}