{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T05:51:56Z","timestamp":1790488316775,"version":"4.1.0"},"reference-count":54,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2014,12,1]],"date-time":"2014-12-01T00:00:00Z","timestamp":1417392000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2014,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content content-type=\"genus-species\">Streptococcus pneumoniae<\/jats:named-content>\n            adherence to human epithelial cells (HECs) is the first step in pathogenesis leading to infections. We sought to determine the role of human antibodies against\n            <jats:named-content content-type=\"genus-species\">S. pneumoniae<\/jats:named-content>\n            protein vaccine candidates PhtD, PcpA, and Ply in preventing adherence to lung HECs\n            <jats:italic>in vitro<\/jats:italic>\n            and mouse nasopharyngeal (NP) colonization\n            <jats:italic>in vivo<\/jats:italic>\n            . Human anti-PhtD, -PcpA, and -Ply antibodies were purified and Fab fragments generated. Fabs were used to test inhibition of adherence of TIGR4 and nonencapsulated strain RX1 to A549 lung HECs. The roles of individual proteins in adherence were tested using isogenic mutants of strain TIGR4. Anti-PhtD, -PcpA, and -Ply human antibodies were assessed for their ability to inhibit NP colonization\n            <jats:italic>in vivo<\/jats:italic>\n            by passive transfer of human antibody in a murine model. Human antibodies generated against PhtD and PcpA caused a decrease in adherence to A549 cells (\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.05). Anti-PhtD but not anti-PcpA antibodies showed a protective role against mouse NP colonization. To our surprise, anti-Ply antibodies also caused a significant (\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.05) reduction in\n            <jats:named-content content-type=\"genus-species\">S. pneumoniae<\/jats:named-content>\n            colonization. Our results support the potential of PhtD, PcpA, and Ply protein vaccine candidates as alternatives to conjugate vaccines to prevent non-serotype-specific\n            <jats:named-content content-type=\"genus-species\">S. pneumoniae<\/jats:named-content>\n            colonization and invasive infection.\n          <\/jats:p>","DOI":"10.1128\/iai.02124-14","type":"journal-article","created":{"date-parts":[[2014,9,23]],"date-time":"2014-09-23T03:28:01Z","timestamp":1411442881000},"page":"5069-5075","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":51,"title":["Human Antibodies to PhtD, PcpA, and Ply Reduce Adherence to Human Lung Epithelial Cells and Murine Nasopharyngeal Colonization by Streptococcus pneumoniae"],"prefix":"10.1128","volume":"82","author":[{"given":"Ravinder","family":"Kaur","sequence":"first","affiliation":[{"name":"Center for Infectious Diseases and Immunology, Rochester General Hospital Research Institute, Rochester, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naveen","family":"Surendran","sequence":"additional","affiliation":[{"name":"Center for Infectious Diseases and Immunology, Rochester General Hospital Research Institute, Rochester, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martina","family":"Ochs","sequence":"additional","affiliation":[{"name":"Sanofi Pasteur, Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael E.","family":"Pichichero","sequence":"additional","affiliation":[{"name":"Center for Infectious Diseases and Immunology, Rochester General Hospital Research Institute, Rochester, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1053\/jinf.2000.0653"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(04)00938-7"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pcl.2012.12.007"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1871"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00562-13"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(10)62225-8"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00807-13"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.3109\/1040841X.2013.813902"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2012.02.023"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1097\/INF.0b013e31821c2d8b"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2014.04.004"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1096\/fj.08-119537"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi200012f"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.2.949-958.2001"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00050-09"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.4161\/hv.19820"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.12.6533-6542.1999"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01126-07"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.74.2.1171-1180.2006"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2002.03106.x"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1998.tb13087.x"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2013.11.096"},{"key":"e_1_3_3_24_2","volume-title":"In Abstr. 54th Intersci. 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