{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T04:55:10Z","timestamp":1790744110631,"version":"4.1.0"},"reference-count":48,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2015,2,1]],"date-time":"2015-02-01T00:00:00Z","timestamp":1422748800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2015,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Accumulation of\n            <jats:named-content content-type=\"genus-species\">Tropheryma whipplei<\/jats:named-content>\n            -stuffed macrophages in the duodenum, impaired\n            <jats:named-content content-type=\"genus-species\">T. whipplei<\/jats:named-content>\n            -specific Th1 responses, and weak secretion of interleukin-12 (IL-12) are hallmarks of classical Whipple's disease (CWD). This study addresses dendritic cell (DC) functionality during CWD. We documented composition, distribution, and functionality of DC\n            <jats:italic>ex vivo<\/jats:italic>\n            or after\n            <jats:italic>in vitro<\/jats:italic>\n            maturation by fluorescence-activated cell sorting (FACS) and by immunohistochemistry\n            <jats:italic>in situ<\/jats:italic>\n            . A decrease in peripheral DC of untreated CWD patients compared to healthy donors was due to reduced CD11c\n            <jats:sup>high<\/jats:sup>\n            myeloid DC (M-DC). Decreased maturation markers CD83, CD86, and CCR7, as well as low IL-12 production in response to stimulation, disclosed an immature M-DC phenotype.\n            <jats:italic>In vitro<\/jats:italic>\n            -generated monocyte-derived DC from CWD patients showed normal maturation and T cell-stimulatory capacity under proinflammatory conditions but produced less IL-12 and failed to activate\n            <jats:named-content content-type=\"genus-species\">T. whipplei<\/jats:named-content>\n            -specific Th1 cells. In duodenal and lymphoid tissues,\n            <jats:named-content content-type=\"genus-species\">T. whipplei<\/jats:named-content>\n            was found within immature DC-SIGN\n            <jats:sup>+<\/jats:sup>\n            DC. DC and proliferating lymphocytes were reduced in lymph nodes of CWD patients compared to levels in controls. Our results indicate that dysfunctional IL-12 production by DC provides suboptimal conditions for priming of\n            <jats:named-content content-type=\"genus-species\">T. whipplei<\/jats:named-content>\n            -specific T cells during CWD and that immature DC carrying\n            <jats:named-content content-type=\"genus-species\">T. whipplei<\/jats:named-content>\n            contribute to the dissemination of the bacterium.\n          <\/jats:p>","DOI":"10.1128\/iai.02463-14","type":"journal-article","created":{"date-parts":[[2014,11,11]],"date-time":"2014-11-11T05:20:18Z","timestamp":1415683218000},"page":"482-491","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":22,"title":["Role of Dendritic Cells in the Pathogenesis of Whipple's Disease"],"prefix":"10.1128","volume":"83","author":[{"given":"Katina","family":"Schinnerling","sequence":"first","affiliation":[{"name":"Medizinische Klinik I, 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