{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,9,15]],"date-time":"2024-09-15T22:20:53Z","timestamp":1726438853626},"reference-count":106,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2015,1,1]],"date-time":"2015-01-01T00:00:00Z","timestamp":1420070400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2015,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            There are a paucity of data concerning gene products that could contribute to the ability of\n            <jats:named-content content-type=\"genus-species\">Moraxella catarrhalis<\/jats:named-content>\n            to colonize the human nasopharynx. Inactivation of a gene (\n            <jats:italic>mesR<\/jats:italic>\n            ) encoding a predicted response regulator of a two-component signal transduction system in\n            <jats:named-content content-type=\"genus-species\">M. catarrhalis<\/jats:named-content>\n            yielded a mutant unable to grow in liquid media. This\n            <jats:italic>mesR<\/jats:italic>\n            mutant also exhibited increased sensitivity to certain stressors, including polymyxin B, SDS, and hydrogen peroxide. Inactivation of the gene (\n            <jats:italic>mesS<\/jats:italic>\n            ) encoding the predicted cognate sensor (histidine) kinase yielded a mutant with the same inability to grow in liquid media as the\n            <jats:italic>mesR<\/jats:italic>\n            mutant. DNA microarray and real-time reverse transcriptase PCR analyses indicated that several genes previously shown to be involved in the ability of\n            <jats:named-content content-type=\"genus-species\">M. catarrhalis<\/jats:named-content>\n            to persist in the chinchilla nasopharynx were upregulated in the\n            <jats:italic>mesR<\/jats:italic>\n            mutant. Two other open reading frames upregulated in the\n            <jats:italic>mesR<\/jats:italic>\n            mutant were shown to encode small proteins (LipA and LipB) that had amino acid sequence homology to bacterial adhesins and structural homology to bacterial lysozyme inhibitors. Inactivation of both\n            <jats:italic>lipA<\/jats:italic>\n            and\n            <jats:italic>lipB<\/jats:italic>\n            did not affect the ability of\n            <jats:named-content content-type=\"genus-species\">M. catarrhalis<\/jats:named-content>\n            O35E to attach to a human bronchial epithelial cell line\n            <jats:italic>in vitro<\/jats:italic>\n            . Purified recombinant LipA and LipB fusion proteins were each shown to inhibit human lysozyme activity\n            <jats:italic>in vitro<\/jats:italic>\n            and in saliva. A\n            <jats:italic>lipA lipB<\/jats:italic>\n            deletion mutant was more sensitive than the wild-type parent strain to killing by human lysozyme in the presence of human apolactoferrin. This is the first report of the production of lysozyme inhibitors by\n            <jats:named-content content-type=\"genus-species\">M. catarrhalis<\/jats:named-content>\n            .\n          <\/jats:p>","DOI":"10.1128\/iai.02486-14","type":"journal-article","created":{"date-parts":[[2014,10,14]],"date-time":"2014-10-14T03:19:53Z","timestamp":1413256793000},"page":"146-160","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":5,"title":["A Moraxella catarrhalis Two-Component Signal Transduction System Necessary for Growth in Liquid Media Affects Production of Two Lysozyme Inhibitors"],"prefix":"10.1128","volume":"83","author":[{"given":"Stephanie N.","family":"Joslin","sequence":"first","affiliation":[{"name":"Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, Texas, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christine","family":"Pybus","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, Texas, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria","family":"Labandeira-Rey","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, Texas, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amanda S.","family":"Evans","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, University of Texas Southwestern Medical Center, Dallas, Texas, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ahmed S.","family":"Attia","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, Texas, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chad A.","family":"Brautigam","sequence":"additional","affiliation":[{"name":"Department of Biophysics, University of Texas Southwestern Medical Center, Dallas, Texas, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eric J.","family":"Hansen","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, Texas, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","first-page":"2529","volume-title":"Mandell, Douglas, and Bennett's principles and practice of infectious disease","author":"Murphy TF","year":"2005","unstructured":"Murphy TF. 2005. Moraxella (Branhamella) catarrhalis and other gram-negative cocci, p 2529. In Mandell GL, Bennett JE, Dolin R (ed), Mandell, Douglas, and Bennett's principles and practice of infectious disease, 6th ed. Elsevier Inc., Philadelphia, PA.","edition":"6"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1286-4579(00)00314-2"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1097\/00006454-200005001-00003"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.15.1.125-144.2002"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/mr.60.2.267-279.1996"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.200412-1747OC"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa012561"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.06.00024505"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1086\/599375"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/169.6.1312"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.296.2.202"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijporl.2009.05.016"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.6.3302-3309.2003"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.9.4977-4984.2003"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.182.5.1364-1373.2000"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0194-5998(97)70321-8"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.4.1906-1913.2004"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00946-07"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01330-06"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00396-07"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.05918-11"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01745-14"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.183.5.1540-1551.2001"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.8.4523-4533.2002"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00609-06"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.185.7.2285-2295.2003"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-695X.2002.tb00548.x"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01204-06"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.4.1843-1848.2003"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0068298"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01060-10"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01032-08"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-695X.1998.tb01225.x"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00073-07"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00121-10"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-12-70"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01833-12"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1165\/ajrcmb.10.1.7507342"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01347-06"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(89)90358-2"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.175.18.5899-5906.1993"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.plasmid.2006.03.002"},{"key":"e_1_3_3_44_2","first-page":"528","article-title":"Gene splicing by overlap extension: tailor-made genes using the polymerase chain reaction","volume":"8","author":"Horton RM","year":"1990","unstructured":"Horton RM, Cai Z, Ho SN, Pease LR. 1990. Gene splicing by overlap extension: tailor-made genes using the polymerase chain reaction. Biotechniques 8:528\u2013535.","journal-title":"Biotechniques"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.173.18.5685-5693.1991"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.61.5.2003-2010.1993"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1038\/msb4100050"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000019"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.117.2.696-701.1974"},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/16.4.404"},{"key":"e_1_3_3_51_2","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.2000.4315"},{"key":"e_1_3_3_52_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkn072"},{"key":"e_1_3_3_53_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.emboj.7600886"},{"key":"e_1_3_3_54_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2005.02.013"},{"key":"e_1_3_3_55_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01534-10"},{"key":"e_1_3_3_56_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0969-2126(02)00761-X"},{"key":"e_1_3_3_57_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2009.06931.x"},{"key":"e_1_3_3_58_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.06357-11"},{"key":"e_1_3_3_59_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00079-07"},{"key":"e_1_3_3_60_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01039-08"},{"key":"e_1_3_3_61_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(05)80360-2"},{"key":"e_1_3_3_62_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-14-30"},{"key":"e_1_3_3_63_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1119(96)00639-7"},{"key":"e_1_3_3_64_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00041-13"},{"key":"e_1_3_3_65_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gki408"},{"key":"e_1_3_3_66_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2010.12.007"},{"key":"e_1_3_3_67_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2008.11.039"},{"key":"e_1_3_3_68_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10858-006-9032-y"},{"key":"e_1_3_3_69_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi401241c"},{"key":"e_1_3_3_70_2","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1999.3091"},{"key":"e_1_3_3_71_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.7.3113-3119.1998"},{"key":"e_1_3_3_72_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2012.06.005"},{"key":"e_1_3_3_73_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI115407"},{"key":"e_1_3_3_74_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-004-4066-3"},{"key":"e_1_3_3_75_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.56.11.2774-2781.1988"},{"key":"e_1_3_3_76_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110.195412"},{"key":"e_1_3_3_77_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2012.02645.x"},{"key":"e_1_3_3_78_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.181.21.6772-6778.1999"},{"key":"e_1_3_3_79_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00798-08"},{"key":"e_1_3_3_80_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4614-3561-7_40"},{"key":"e_1_3_3_81_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01745-14"},{"key":"e_1_3_3_82_2","doi-asserted-by":"publisher","DOI":"10.1128\/9781555818319"},{"key":"e_1_3_3_83_2","doi-asserted-by":"publisher","DOI":"10.1038\/nri3089"},{"key":"e_1_3_3_84_2","doi-asserted-by":"publisher","DOI":"10.4161\/viru.1.5.12983"},{"key":"e_1_3_3_85_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12038-010-0015-5"},{"key":"e_1_3_3_86_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.165.10.5760"},{"key":"e_1_3_3_87_2","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.200305-669OC"},{"key":"e_1_3_3_88_2","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.0205073"},{"key":"e_1_3_3_89_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000241"},{"key":"e_1_3_3_90_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2334-8-134"},{"key":"e_1_3_3_91_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.169.12.6985"},{"key":"e_1_3_3_92_2","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2005-0059OC"},{"key":"e_1_3_3_93_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2334-4-12"},{"key":"e_1_3_3_94_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(01)02872-1"},{"key":"e_1_3_3_95_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M106317200"},{"key":"e_1_3_3_96_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.177.1.519"},{"key":"e_1_3_3_97_2","doi-asserted-by":"publisher","DOI":"10.1136\/thx.30.1.72"},{"key":"e_1_3_3_98_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00651-10"},{"key":"e_1_3_3_99_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M010297200"},{"key":"e_1_3_3_100_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0045954"},{"key":"e_1_3_3_101_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jit057"},{"key":"e_1_3_3_102_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00503-13"},{"key":"e_1_3_3_103_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00326.2003"},{"key":"e_1_3_3_104_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00409-12"},{"key":"e_1_3_3_105_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0040088"},{"key":"e_1_3_3_106_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00314-10"},{"key":"e_1_3_3_107_2","doi-asserted-by":"publisher","DOI":"10.1002\/prot.340230412"}],"container-title":["Infection and Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.02486-14","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.02486-14","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T07:42:09Z","timestamp":1646466129000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/IAI.02486-14"}},"subtitle":[],"editor":[{"given":"S. M.","family":"Payne","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2015,1]]},"references-count":106,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2015,1]]}},"alternative-id":["10.1128\/IAI.02486-14"],"URL":"https:\/\/doi.org\/10.1128\/iai.02486-14","relation":{},"ISSN":["0019-9567","1098-5522"],"issn-type":[{"type":"print","value":"0019-9567"},{"type":"electronic","value":"1098-5522"}],"subject":[],"published":{"date-parts":[[2015,1]]},"assertion":[{"value":"2014-08-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-10-09","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-12-16","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}