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We examined the potential of EhMSP-1 for use as a vaccine antigen to protect against amebic liver abscesses. First, existing serum samples from South Africans naturally infected with<jats:named-content content-type=\"genus-species\">E. histolytica<\/jats:named-content>were examined by enzyme-linked immunosorbent assay (ELISA) for the presence of EhMSP-1-specific IgG. Nine of 12 (75%) people with anti-<jats:named-content content-type=\"genus-species\">E. histolytica<\/jats:named-content>IgG also had EhMSP-1-specific IgG antibodies. We next used a hamster model of amebic liver abscess to determine the effect of immunization with a mixture of four recombinant EhMSP-1 protein fragments. EhMSP-1 immunization stimulated a robust IgG antibody response. Furthermore, EhMSP-1 immunization of hamsters reduced development of severe amebic liver abscesses following intrahepatic injection of<jats:named-content content-type=\"genus-species\">E. histolytica<\/jats:named-content>by a combined rate of 68% in two independent animal experiments. Purified IgG from immunized compared to control animals bound to the surface of<jats:named-content content-type=\"genus-species\">E. histolytica<\/jats:named-content>trophozoites and accelerated amebic lysis via activation of the classical complement cascade. We concluded that EhMSP-1 is a promising antigen that warrants further study to determine its full potential as a target for therapy and\/or prevention of invasive amebiasis.<\/jats:p>","DOI":"10.1128\/iai.02490-14","type":"journal-article","created":{"date-parts":[[2014,12,2]],"date-time":"2014-12-02T08:05:56Z","timestamp":1417507556000},"page":"713-720","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":17,"title":["Immunization with the Entamoeba histolytica Surface Metalloprotease EhMSP-1 Protects Hamsters from Amebic Liver Abscess"],"prefix":"10.1128","volume":"83","author":[{"given":"Eduardo C.","family":"Roncolato","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Immunology, Ribeir\u00e3o Preto Medical School, University of S\u00e3o Paulo, Ribeir\u00e3o Preto, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 E.","family":"Teixeira","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Vermont, Burlington, Vermont, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 E.","family":"Barbosa","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Immunology, Ribeir\u00e3o Preto Medical School, University of S\u00e3o Paulo, Ribeir\u00e3o Preto, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leandra N.","family":"Zambelli Ramalho","sequence":"additional","affiliation":[{"name":"Department of Pathology and Legal Medicine, Ribeir\u00e3o Preto Medical School, University of S\u00e3o Paulo, Ribeir\u00e3o Preto, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher D.","family":"Huston","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Molecular Genetics, University of Vermont, Burlington, Vermont, USA"},{"name":"Department of Medicine, University of Vermont, Burlington, Vermont, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(10)60549-1"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(13)60844-2"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cis1044"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1999.60.1031"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jit507"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra022710"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.10.4.637"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1086\/320740"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.74.2.904-909.2006"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1086\/341566"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.62.6.2605-2608.1994"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.186.9.1557"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.63.2.472-477.1995"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.59.1.97-101.1991"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2003.09.003"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.62.4.1166-1170.1994"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2012.02.066"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.06389-11"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-6851(03)00211-1"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2012.03.068"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkp941"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(90)90458-8"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(78)90144-X"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1080\/00034983.1991.11812571"},{"key":"e_1_3_2_26_2","volume-title":"A handbook for high-level expression and purification of 6xHis-tagged proteins","author":"Qiagen","year":"2003","unstructured":"Qiagen. 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