{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T03:03:18Z","timestamp":1784602998262,"version":"3.55.0"},"reference-count":44,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2015,1,1]],"date-time":"2015-01-01T00:00:00Z","timestamp":1420070400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2015,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content content-type=\"genus-species\">Staphylococcus aureus<\/jats:named-content>\n            is a common pathogen found in the community and in hospitals. Most notably, methicillin-resistant\n            <jats:named-content content-type=\"genus-species\">S. aureus<\/jats:named-content>\n            is resistant to many antibiotics, which is a growing public health concern. The emergence of drug-resistant strains has prompted the search for alternative treatments, such as immunotherapeutic approaches. To date, most clinical trials of vaccines or of passive immunization against\n            <jats:named-content content-type=\"genus-species\">S. aureus<\/jats:named-content>\n            have ended in failure. In this study, we investigated two ESAT-6-like proteins secreted by\n            <jats:named-content content-type=\"genus-species\">S. aureus<\/jats:named-content>\n            ,\n            <jats:named-content content-type=\"genus-species\">S. aureus<\/jats:named-content>\n            EsxA (SaEsxA) and SaEsxB, as possible targets for a vaccine. Mice vaccinated with these purified proteins elicited high titers of anti-SaEsxA and anti-SaEsxB antibodies, but these antibodies could not prevent\n            <jats:named-content content-type=\"genus-species\">S. aureus<\/jats:named-content>\n            infection. On the other hand, recombinant SaEsxA (rSaEsxA) and rSaEsxB could induce Th1- and Th17-biased immune responses in mice. Mice immunized with rSaEsxA and rSaEsxB had significantly improved survival rates when challenged with\n            <jats:named-content content-type=\"genus-species\">S. aureus<\/jats:named-content>\n            compared with the controls. These findings indicate that SaEsxA and SaEsxB are two promising Th1 and Th17 candidate antigens which could be developed into multivalent and serotype-independent vaccines against\n            <jats:named-content content-type=\"genus-species\">S. aureus<\/jats:named-content>\n            infection.\n          <\/jats:p>","DOI":"10.1128\/iai.02498-14","type":"journal-article","created":{"date-parts":[[2014,11,4]],"date-time":"2014-11-04T03:33:32Z","timestamp":1415072012000},"page":"339-345","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":24,"title":["Recombinant ESAT-6-Like Proteins Provoke Protective Immune Responses against Invasive Staphylococcus aureus Disease in a Murine Model"],"prefix":"10.1128","volume":"83","author":[{"given":"Bao Zhong","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Biochemistry, The University of Hong Kong, Pokfulam, Hong Kong, China"},{"name":"Department of Microbiology, The University of Hong Kong, Pokfulam, Hong Kong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan Hong","family":"Hua","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, The University of Hong Kong, Pokfulam, Hong Kong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, The University of Hong Kong, Pokfulam, Hong Kong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Candy Choi Yi","family":"Lau","sequence":"additional","affiliation":[{"name":"Department of Microbiology, The University of Hong Kong, Pokfulam, Hong Kong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian Piao","family":"Cai","sequence":"additional","affiliation":[{"name":"Department of Microbiology, The University of Hong Kong, Pokfulam, Hong Kong, 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