{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T23:58:54Z","timestamp":1790812734493,"version":"4.1.0"},"reference-count":47,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2014,12,1]],"date-time":"2014-12-01T00:00:00Z","timestamp":1417392000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2014,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Hydrosalpinx induction in mice by\n            <jats:named-content content-type=\"genus-species\">Chlamydia muridarum<\/jats:named-content>\n            infection, a model that has been used to study\n            <jats:named-content content-type=\"genus-species\">C. trachomatis<\/jats:named-content>\n            pathogenesis in women, is known to depend on the cryptic plasmid that encodes eight genes designated\n            <jats:italic>pgp1<\/jats:italic>\n            to\n            <jats:italic>pgp8<\/jats:italic>\n            . To identify the plasmid-encoded pathogenic determinants, we evaluated\n            <jats:named-content content-type=\"genus-species\">C. muridarum<\/jats:named-content>\n            transformants deficient in the plasmid-borne gene\n            <jats:italic>pgp3<\/jats:italic>\n            , -\n            <jats:italic>4<\/jats:italic>\n            , or -\n            <jats:italic>7<\/jats:italic>\n            for induction of hydrosalpinx.\n            <jats:named-content content-type=\"genus-species\">C. muridarum<\/jats:named-content>\n            transformants with an in-frame deletion of either\n            <jats:italic>pgp3<\/jats:italic>\n            or -\n            <jats:italic>4<\/jats:italic>\n            but not -\n            <jats:italic>7<\/jats:italic>\n            failed to induce hydrosalpinx. The deletion mutant phenotype was reproduced by using transformants with premature termination codon insertions in the corresponding\n            <jats:italic>pgp<\/jats:italic>\n            genes (to minimize polar effects inherent in the deletion mutants). Pgp4 is known to regulate\n            <jats:italic>pgp3<\/jats:italic>\n            expression, while lack of Pgp3 does not significantly affect Pgp4 function. Thus, we conclude that Pgp3 is an effector virulence factor and that lack of Pgp3 may be responsible for the attenuation in\n            <jats:named-content content-type=\"genus-species\">C. muridarum<\/jats:named-content>\n            pathogenicity described above. This attenuated pathogenicity was further correlated with a rapid decrease in chlamydial survival in the lower genital tract and reduced ascension to the upper genital tract in mice infected with\n            <jats:named-content content-type=\"genus-species\">C. muridarum<\/jats:named-content>\n            deficient in Pgp3 but not Pgp7. The Pgp3-deficient\n            <jats:named-content content-type=\"genus-species\">C. muridarum<\/jats:named-content>\n            organisms were also less invasive when delivered directly to the oviduct on day 7 after inoculation. These observations demonstrate that plasmid-encoded Pgp3 is required for\n            <jats:named-content content-type=\"genus-species\">C. muridarum<\/jats:named-content>\n            survival in the mouse genital tract and represents a major virulence factor in\n            <jats:named-content content-type=\"genus-species\">C. muridarum<\/jats:named-content>\n            pathogenesis in mice.\n          <\/jats:p>","DOI":"10.1128\/iai.02576-14","type":"journal-article","created":{"date-parts":[[2014,10,7]],"date-time":"2014-10-07T07:41:35Z","timestamp":1412667695000},"page":"5327-5335","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":124,"title":["Plasmid-Encoded Pgp3 Is a Major Virulence Factor for Chlamydia muridarum To Induce Hydrosalpinx in Mice"],"prefix":"10.1128","volume":"82","author":[{"given":"Yuanjun","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Microbiology &amp; Immunology, University of Texas Health Science Center at San Antonio, San Antonio, Texas, USA"},{"name":"Department of Dermatovenereology, Tianjin Medical University General Hospital, Tianjin, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yumeng","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Microbiology &amp; Immunology, University of Texas Health Science Center at San Antonio, San Antonio, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhangsheng","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Microbiology &amp; Immunology, University of Texas Health Science Center at San Antonio, San Antonio, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yina","family":"Sun","sequence":"additional","affiliation":[{"name":"Key Laboratory of Hormones and Development (Ministry of Health), Metabolic Diseases Hospital and Tianjin Institute of Endocrinology, Tianjin Medical University, Tianjin, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siqi","family":"Gong","sequence":"additional","affiliation":[{"name":"Department of Microbiology &amp; Immunology, University of Texas Health Science Center at San Antonio, San Antonio, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuping","family":"Hou","sequence":"additional","affiliation":[{"name":"Department of Dermatovenereology, Tianjin Medical University General Hospital, Tianjin, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chaoqun","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of South China, Hengyang, Hunan, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhongyu","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of South China, Hengyang, Hunan, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Quanzhong","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Dermatovenereology, Tianjin Medical University General Hospital, Tianjin, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yimou","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of South China, Hengyang, Hunan, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joel","family":"Baseman","sequence":"additional","affiliation":[{"name":"Department of Microbiology &amp; Immunology, University of Texas Health Science Center at San Antonio, San Antonio, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangming","family":"Zhong","sequence":"additional","affiliation":[{"name":"Department of Microbiology &amp; Immunology, University of Texas Health Science Center at San Antonio, San Antonio, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-0997(1998)6:4<163::AID-IDOG5>3.0.CO;2-6"},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2011.06.021"},{"key":"e_1_3_1_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.6.2741-2751.2002"},{"key":"e_1_3_1_5_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.olq.0000148299.14513.11"},{"key":"e_1_3_1_6_2","first-page":"980","article-title":"Vaccines for Chlamydia trachomatis infections","volume":"3","author":"de la Maza LM","year":"2002","unstructured":"de la MazaLMPetersonEM. 2002. 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