{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T00:53:54Z","timestamp":1774400034905,"version":"3.50.1"},"reference-count":41,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2015,3,1]],"date-time":"2015-03-01T00:00:00Z","timestamp":1425168000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2015,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            In\n            <jats:named-content content-type=\"genus-species\">Aspergillus nidulans<\/jats:named-content>\n            , the AcuK and AcuM transcription factors form a complex that regulates gluconeogenesis. In\n            <jats:named-content content-type=\"genus-species\">Aspergillus fumigatus<\/jats:named-content>\n            , AcuM governs gluconeogenesis and iron acquisition\n            <jats:italic>in vitro<\/jats:italic>\n            and virulence in immunosuppressed mice. However, the function of AcuK was previously unknown. Through\n            <jats:italic>in vitro<\/jats:italic>\n            studies, we found that\n            <jats:named-content content-type=\"genus-species\">A. fumigatus<\/jats:named-content>\n            \u0394\n            <jats:italic>acuK<\/jats:italic>\n            single and \u0394\n            <jats:italic>acuK<\/jats:italic>\n            \u0394\n            <jats:italic>acuM<\/jats:italic>\n            double mutants had impaired gluconeogenesis and iron acquisition, similar to the \u0394\n            <jats:italic>acuM<\/jats:italic>\n            mutant. Also, the \u0394\n            <jats:italic>acuK<\/jats:italic>\n            , \u0394\n            <jats:italic>acuM<\/jats:italic>\n            , and \u0394\n            <jats:italic>acuK<\/jats:italic>\n            \u0394\n            <jats:italic>acuM<\/jats:italic>\n            mutants had similar virulence defects in mice. However, the \u0394\n            <jats:italic>acuK<\/jats:italic>\n            mutant had a milder defect in extracellular siderophore activity and induction of epithelial cell damage\n            <jats:italic>in vitro<\/jats:italic>\n            than did the \u0394\n            <jats:italic>acuM<\/jats:italic>\n            mutant. Moreover, overexpression of\n            <jats:italic>acuM<\/jats:italic>\n            in the \u0394\n            <jats:italic>acuK<\/jats:italic>\n            mutant altered expression of 3 genes and partially restored growth under iron-limited conditions, suggesting that AcuM can govern some genes independently of AcuK. Although the \u0394\n            <jats:italic>acuK<\/jats:italic>\n            and \u0394\n            <jats:italic>acuM<\/jats:italic>\n            mutants had very similar transcriptional profiles\n            <jats:italic>in vitro<\/jats:italic>\n            , their transcriptional profiles during murine pulmonary infection differed both from their\n            <jats:italic>in vitro<\/jats:italic>\n            profiles and from each other. While AcuK and AcuM governed the expression of only a few iron-responsive genes\n            <jats:italic>in vivo<\/jats:italic>\n            , they influenced the expression of other virulence-related genes, such as\n            <jats:italic>hexA<\/jats:italic>\n            and\n            <jats:italic>dvrA<\/jats:italic>\n            . Therefore, in\n            <jats:named-content content-type=\"genus-species\">A. fumigatus<\/jats:named-content>\n            , while AcuK and AcuM likely function as part of the same complex, they can also function independently of each other. Furthermore, AcuK and AcuM have different target genes\n            <jats:italic>in vivo<\/jats:italic>\n            than\n            <jats:italic>in vitro<\/jats:italic>\n            , suggesting that\n            <jats:italic>in vivo<\/jats:italic>\n            infection stimulates unique transcriptional regulatory pathways in\n            <jats:named-content content-type=\"genus-species\">A. fumigatus<\/jats:named-content>\n            .\n          <\/jats:p>","DOI":"10.1128\/iai.02685-14","type":"journal-article","created":{"date-parts":[[2014,12,23]],"date-time":"2014-12-23T03:10:36Z","timestamp":1419304236000},"page":"923-933","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":38,"title":["Divergent Targets of Aspergillus fumigatus AcuK and AcuM Transcription Factors during Growth\n            <i>In Vitro<\/i>\n            versus Invasive Disease"],"prefix":"10.1128","volume":"83","author":[{"given":"Monsicha","family":"Pongpom","sequence":"first","affiliation":[{"name":"Department of Microbiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong","family":"Liu","sequence":"additional","affiliation":[{"name":"Los Angeles Biomedical Research Institute at Harbor-UCLA Medical Center, Torrance, California, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenjie","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, Pennsylvania, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brendan D.","family":"Snarr","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology and Department of Medicine, McGill University, Montreal, Quebec, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Donald C.","family":"Sheppard","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology and Department of Medicine, McGill University, Montreal, Quebec, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aaron P.","family":"Mitchell","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, Pennsylvania, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Scott G.","family":"Filler","sequence":"additional","affiliation":[{"name":"Los Angeles Biomedical Research Institute at Harbor-UCLA Medical Center, Torrance, California, USA"},{"name":"David Geffen School of Medicine at UCLA, Los Angeles, California, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/EC.00274-07"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.12.2.310"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1086\/651262"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1086\/595846"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2334-13-29"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.coi.2009.01.011"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mycres.2007.11.012"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagen.2008.07.002"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2012.00028"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2011.06.002"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20041242"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.0030128"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.9.5493-5503.2005"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2010.07389.x"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.107.070904"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2012.08067.x"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E05-07-0617"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(87)90164-8"},{"key":"e_1_3_3_20_2","first-page":"9","article-title":"Split-marker recombination for efficient targeted deletion of fungal genes","volume":"50","author":"Catlett NL","year":"2003","unstructured":"Catlett NL, Lee B-N, Yoder OC, Turgeon BG. 2003. 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