{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,5]],"date-time":"2025-10-05T16:52:52Z","timestamp":1759683172382},"reference-count":102,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2015,3,1]],"date-time":"2015-03-01T00:00:00Z","timestamp":1425168000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2015,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Both the endogenous antisecretory factor (AF) protein and peptide AF-16, which has a sequence that matches that of the active N-terminal region of AF, inhibit the increase in the epithelial transport of fluid and electrolytes induced by bacterial toxins in animal and\n            <jats:italic>ex vivo<\/jats:italic>\n            models. We conducted a study to investigate the inhibitory effect of peptide AF-16 against the increase of transcellular passage and paracellular permeability promoted by the\n            <jats:underline>s<\/jats:underline>\n            ecreted\n            <jats:underline>a<\/jats:underline>\n            utotransporter\n            <jats:underline>t<\/jats:underline>\n            oxin (Sat) in a cultured cellular model of the human intestinal epithelial barrier. Peptide AF-16 produced a concentration-dependent inhibition of the Sat-induced increase in the formation of fluid domes, in the mucosal-to-serosal passage of\n            <jats:sc>d<\/jats:sc>\n            -[1-\n            <jats:sup>14<\/jats:sup>\n            C]mannitol, and in the rearrangements in the distribution and protein expression of the tight junction (TJ)-associated proteins ZO-1 and occludin in cultured human enterocyte-like Caco-2\/TC7 cell monolayers. In addition, we show that peptide AF-16 also inhibits the cholera toxin-induced increase of transcellular passage and the\n            <jats:named-content content-type=\"genus-species\">Clostridium difficile<\/jats:named-content>\n            toxin-induced effects on paracellular permeability and TJ protein organization in Caco-2\/TC7 cell monolayers. Treatment of cell monolayers by the lipid raft disorganizer methyl-\u03b2-cyclodextrin abolished the inhibitory activity of peptide AF-16 at the transcellular passage level and did not modify the effect of the peptide at the paracellular level.\n          <\/jats:p>","DOI":"10.1128\/iai.02759-14","type":"journal-article","created":{"date-parts":[[2014,12,23]],"date-time":"2014-12-23T03:10:36Z","timestamp":1419304236000},"page":"907-922","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":6,"title":["Antisecretory Factor Peptide AF-16 Inhibits the Secreted Autotransporter Toxin-Stimulated Transcellular and Paracellular Passages of Fluid in Cultured Human Enterocyte-Like Cells"],"prefix":"10.1128","volume":"83","author":[{"given":"Val\u00e9rie","family":"Nicolas","sequence":"first","affiliation":[{"name":"IFR 141 IPSIT, Plateforme Imagerie Cellulaire, Facult\u00e9 de Pharmacie, Ch\u00e2tenay-Malabry, France"},{"name":"Universit\u00e9 Paris-Sud, Facult\u00e9 de Pharmacie, Ch\u00e2tenay-Malabry, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vanessa","family":"Li\u00e9vin-Le Moal","sequence":"additional","affiliation":[{"name":"CNRS, UMR 8076 BioCIS, Facult\u00e9 de Pharmacie, Ch\u00e2tenay-Malabry, France"},{"name":"LabEx LERMIT\u2014Laboratory of Excellence in Research on Medication and Innovative Therapeutics, Ch\u00e2tenay-Malabry, France"},{"name":"Universit\u00e9 Paris-Sud, Facult\u00e9 de Pharmacie, Ch\u00e2tenay-Malabry, France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(10)60549-1"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/nri2653"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00022-13"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.autneu.2006.10.005"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0954422410000193"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4165(86)90144-3"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.35.20615"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0463.2006.apm_464.x"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/labinvest.3700036"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1007\/s004410051322"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.nut.2010.09.009"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1136\/gut.46.6.824"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0261-5614(03)00057-8"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0463.2003.1111009.x"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1136\/gut.32.7.791"},{"key":"e_1_3_2_17_2","first-page":"7","article-title":"Antisecretory activity in a lectin fraction of plasma from patients with acute diarrhea","volume":"24","author":"Torres J","year":"1993","unstructured":"Torres J, Lonroth I, Lange S, Camorlinga-Ponce M, Gonzalez-Arroyo S, Munoz O. 1993. Antisecretory activity in a lectin fraction of plasma from patients with acute diarrhea. Arch Med Res 24:7\u201311.","journal-title":"Arch Med Res"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0925-4439(97)00066-5"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00359-002-0330-x"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpb.2004.08.001"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-201X.2003.01173.x"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1136\/gut.41.5.642"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1018863315666"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00583485"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/0300-9629(88)90675-5"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0442.1991.tb01005.x"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1139\/o99-043"},{"key":"e_1_3_2_28_2","first-page":"113","article-title":"Plasma level of antisecretory factor (ASF) and its relation to post-weaning diarrhoea in piglets","volume":"40","author":"Lange S","year":"1993","unstructured":"Lange S, Martinsson K, Lonnroth I, Goransson L. 1993. Plasma level of antisecretory factor (ASF) and its relation to post-weaning diarrhoea in piglets. Zentralbl Veterinarmed B 40:113\u2013118.","journal-title":"Zentralbl Veterinarmed B"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00370696"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00581326"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.3109\/00207458908986244"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1651-2227.2007.00488.x"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1111\/apa.12581"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.3390\/toxins2030310"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2008.01.011"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.00064-12"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2000.02110.x"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-013-1355-8"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/374395"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.femsle.2005.07.013"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2006.00782.x"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.6.3431-3442.2000"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2011.01595.x"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.56.5.1057-1060.1988"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.25.1.191-201.1979"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1985.248.5.C410"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1984.247.3.C260"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01086-06"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.7.4067-4078.2003"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1002\/jss.400120210"},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.1016\/0016-5085(92)90085-D"},{"key":"e_1_3_2_52_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI113760"},{"key":"e_1_3_2_53_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109254200"},{"key":"e_1_3_2_54_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.3.1329-1336.2001"},{"key":"e_1_3_2_55_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.10.6310-6317.2001"},{"key":"e_1_3_2_56_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0068547"},{"key":"e_1_3_2_57_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.77.3.853"},{"key":"e_1_3_2_58_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1041210108"},{"key":"e_1_3_2_59_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)68516-9"},{"key":"e_1_3_2_60_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(87)80947-X"},{"key":"e_1_3_2_61_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M606754200"},{"key":"e_1_3_2_62_2","first-page":"2217","article-title":"Biophysics of domes formed by the renal cell line Madin-Darby canine kidney","volume":"43","author":"Tanner C","year":"1984","unstructured":"Tanner C, Frambach DA, Misfeldt DS. 1984. Biophysics of domes formed by the renal cell line Madin-Darby canine kidney. Fed Proc 43:2217\u20132220.","journal-title":"Fed Proc"},{"key":"e_1_3_2_63_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1041130311"},{"key":"e_1_3_2_64_2","doi-asserted-by":"publisher","DOI":"10.1159\/000047927"},{"key":"e_1_3_2_65_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1986.250.5.C682"},{"key":"e_1_3_2_66_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.2001.281.5.C1551"},{"key":"e_1_3_2_67_2","doi-asserted-by":"publisher","DOI":"10.1007\/s002329900561"},{"key":"e_1_3_2_68_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.2001.281.6.C2039"},{"key":"e_1_3_2_69_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00294.2001"},{"key":"e_1_3_2_70_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00432.2003"},{"key":"e_1_3_2_71_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00274.2005"},{"key":"e_1_3_2_72_2","first-page":"G292","article-title":"Development of an endogenous epithelial Na(+)\/H(+) exchanger (NHE3) in three clones of Caco-2 cells","volume":"277","author":"Janecki AJ","year":"1999","unstructured":"Janecki AJ, Montrose MH, Tse CM, de Medina FS, Zweibaum A, Donowitz M. 1999. Development of an endogenous epithelial Na(+)\/H(+) exchanger (NHE3) in three clones of Caco-2 cells. Am J Physiol 277:G292\u2013G305.","journal-title":"Am J Physiol"},{"key":"e_1_3_2_73_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.2001.281.4.G947"},{"key":"e_1_3_2_74_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.90350.2008"},{"key":"e_1_3_2_75_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00263.2008"},{"key":"e_1_3_2_76_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI7192"},{"key":"e_1_3_2_77_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M107768200"},{"key":"e_1_3_2_78_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0509885103"},{"key":"e_1_3_2_79_2","doi-asserted-by":"publisher","DOI":"10.1042\/BST0370955"},{"key":"e_1_3_2_80_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamem.2003.09.005"},{"key":"e_1_3_2_81_2","doi-asserted-by":"publisher","DOI":"10.1080\/09687860500445604"},{"key":"e_1_3_2_82_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi060570x"},{"key":"e_1_3_2_83_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.2001.00537.x"},{"key":"e_1_3_2_84_2","doi-asserted-by":"publisher","DOI":"10.1159\/000356577"},{"key":"e_1_3_2_85_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00039.2010"},{"key":"e_1_3_2_86_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamem.2008.10.028"},{"key":"e_1_3_2_87_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2053"},{"key":"e_1_3_2_88_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.2012.06528.x"},{"key":"e_1_3_2_89_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.2012.06555.x"},{"key":"e_1_3_2_90_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.113.10.1771"},{"key":"e_1_3_2_91_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M106013200"},{"key":"e_1_3_2_92_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2009.10.002"},{"key":"e_1_3_2_93_2","doi-asserted-by":"publisher","DOI":"10.1080\/09687680410001663267"},{"key":"e_1_3_2_94_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01515-06"},{"key":"e_1_3_2_95_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.2.1053-1060.2001"},{"key":"e_1_3_2_96_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.2007\/007088-0"},{"key":"e_1_3_2_97_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2010.01.043"},{"key":"e_1_3_2_98_2","doi-asserted-by":"publisher","DOI":"10.3390\/toxins4010015"},{"key":"e_1_3_2_99_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00442-07"},{"key":"e_1_3_2_100_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2010.05.009"},{"key":"e_1_3_2_101_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2010.03.016"},{"key":"e_1_3_2_102_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00995-13"},{"key":"e_1_3_2_103_2","doi-asserted-by":"publisher","DOI":"10.1021\/jm4002346"}],"container-title":["Infection and Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.02759-14","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.02759-14","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T12:44:59Z","timestamp":1646484299000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/IAI.02759-14"}},"subtitle":[],"editor":[{"given":"S. R.","family":"Blanke","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2015,3]]},"references-count":102,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2015,3]]}},"alternative-id":["10.1128\/IAI.02759-14"],"URL":"https:\/\/doi.org\/10.1128\/iai.02759-14","relation":{},"ISSN":["0019-9567","1098-5522"],"issn-type":[{"value":"0019-9567","type":"print"},{"value":"1098-5522","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,3]]},"assertion":[{"value":"2014-10-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-12-16","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-02-13","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}