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Evidence suggests that<jats:named-content content-type=\"genus-species\">V. cholerae<\/jats:named-content>O1 may activate inflammatory pathways, and a recent study of a Bangladeshi population showed that variants in innate immune genes play a role in mediating susceptibility to cholera. We analyzed human proteins present in the small intestine of patients infected with<jats:named-content content-type=\"genus-species\">V. cholerae<\/jats:named-content>O1 to characterize the host response to this pathogen. We collected duodenal biopsy specimens from patients with acute cholera after stabilization and again 30 days after initial presentation. Peptides extracted from biopsy specimens were sequenced and quantified using label-free mass spectrometry and SEQUEST. Twenty-seven host proteins were differentially abundant between the acute and convalescent stages of infection; the majority of these have known roles in innate defense, cytokine production, and apoptosis. Immunostaining confirmed that two proteins, WARS and S100A8, were more abundant in lamina propria cells during the acute stage of cholera. Analysis of the differentially abundant proteins revealed the activation of key regulators of inflammation by the innate immune system, including Toll-like receptor 4, nuclear factor kappa-light-chain-enhancer of activated B cells, mitogen-activated protein kinases, and caspase-dependent inflammasomes. Interleukin-12\u03b2 (IL-12\u03b2) was a regulator of several proteins that were activated during cholera, and we confirmed that IL-12\u03b2 was produced by lymphocytes recovered from duodenal biopsy specimens of cholera patients. Our study shows that a broad inflammatory response is generated in the gut early after onset of cholera, which may be critical in the development of long-term mucosal immunity against<jats:named-content content-type=\"genus-species\">V. cholerae<\/jats:named-content>O1.<\/jats:p>","DOI":"10.1128\/iai.02765-14","type":"journal-article","created":{"date-parts":[[2015,1,6]],"date-time":"2015-01-06T04:06:37Z","timestamp":1420517197000},"page":"1089-1103","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":60,"title":["Comparative Proteomic Analysis Reveals Activation of Mucosal Innate Immune Signaling Pathways during Cholera"],"prefix":"10.1128","volume":"83","author":[{"given":"Crystal N.","family":"Ellis","sequence":"first","affiliation":[{"name":"Division of Infectious Diseases, Massachusetts General Hospital, Boston, Massachusetts, USA"},{"name":"Department of Medicine, Harvard Medical School, Boston, Massachusetts, 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