{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T06:02:31Z","timestamp":1782885751273,"version":"3.54.5"},"reference-count":31,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2015,5,1]],"date-time":"2015-05-01T00:00:00Z","timestamp":1430438400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2015,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content content-type=\"genus-species\">Burkholderia pseudomallei<\/jats:named-content>\n            is a CDC tier 1 select agent that causes melioidosis, a severe disease in humans and animals. Persistent infections are common, and there is currently no vaccine available. Lipopolysaccharide (LPS) is a potential vaccine candidate.\n            <jats:named-content content-type=\"genus-species\">B. pseudomallei<\/jats:named-content>\n            expresses three serologically distinct LPS types. The predominant O-polysaccharide (OPS) is an unbranched heteropolymer with repeating\n            <jats:sc>d<\/jats:sc>\n            -glucose and 6-deoxy-\n            <jats:sc>l<\/jats:sc>\n            -talose residues in which the 6-deoxy-\n            <jats:sc>l<\/jats:sc>\n            -talose residues are variably replaced with\n            <jats:italic>O<\/jats:italic>\n            -acetyl and\n            <jats:italic>O<\/jats:italic>\n            -methyl modifications. We observed that primary clinical\n            <jats:named-content content-type=\"genus-species\">B. pseudomallei<\/jats:named-content>\n            isolates with mucoid and nonmucoid colony morphologies from the same sample expressed different antigenic types distinguishable using an LPS-specific monoclonal antibody (MAb). MAb-reactive (nonmucoid) and nonreactive (mucoid) strains from the same patient exhibited identical LPS banding patterns by silver staining and indistinguishable genotypes. We hypothesized that LPS antigenic variation reflected modification of the OPS moieties. Mutagenesis of three genes involved in LPS synthesis was performed in\n            <jats:named-content content-type=\"genus-species\">B. pseudomallei<\/jats:named-content>\n            K96243. Loss of MAb reactivity was observed in both\n            <jats:italic>wbiA<\/jats:italic>\n            (encoding a 2-\n            <jats:italic>O<\/jats:italic>\n            -acetyltransferase) and\n            <jats:italic>wbiD<\/jats:italic>\n            (putative methyl transferase) mutants. The structural characteristics of the OPS moieties from isogenic nonmucoid strain 4095a and mucoid strain 4095c were further investigated. Utilizing nuclear magnetic resonance (NMR) spectroscopy, we found that\n            <jats:named-content content-type=\"genus-species\">B. pseudomallei<\/jats:named-content>\n            4095a and 4095c OPS antigens exhibited substitution patterns that differed from the prototypic OPS structure. Specifically, 4095a lacked 4-\n            <jats:italic>O<\/jats:italic>\n            -acetylation, while 4095c lacked both 4-\n            <jats:italic>O<\/jats:italic>\n            -acetylation and 2-\n            <jats:italic>O<\/jats:italic>\n            -methylation. Our studies indicate that\n            <jats:named-content content-type=\"genus-species\">B. pseudomallei<\/jats:named-content>\n            OPS undergoes antigenic variation and suggest that the 9D5 MAb recognizes a conformational epitope that is influenced by both\n            <jats:italic>O<\/jats:italic>\n            -acetyl and\n            <jats:italic>O<\/jats:italic>\n            -methyl substitution patterns.\n          <\/jats:p>","DOI":"10.1128\/iai.02785-14","type":"journal-article","created":{"date-parts":[[2015,3,17]],"date-time":"2015-03-17T02:12:30Z","timestamp":1426558350000},"page":"2127-2138","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":37,"title":["Colony Morphology Variation of Burkholderia pseudomallei Is Associated with Antigenic Variation and O-Polysaccharide Modification"],"prefix":"10.1128","volume":"83","author":[{"given":"Chanthiwa","family":"Wikraiphat","sequence":"first","affiliation":[{"name":"Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natnaree","family":"Saiprom","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarunporn","family":"Tandhavanant","sequence":"additional","affiliation":[{"name":"Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand"},{"name":"Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Heiss","sequence":"additional","affiliation":[{"name":"Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Parastoo","family":"Azadi","sequence":"additional","affiliation":[{"name":"Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gumphol","family":"Wongsuvan","sequence":"additional","affiliation":[{"name":"Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Apichai","family":"Tuanyok","sequence":"additional","affiliation":[{"name":"Department of Tropical Medicine, Medical Microbiology and Pharmacology, University of Hawaii, Honolulu, Hawaii, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew T. 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