{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T23:42:20Z","timestamp":1791848540720,"version":"4.3.4"},"reference-count":31,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2015,4,1]],"date-time":"2015-04-01T00:00:00Z","timestamp":1427846400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2015,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p><jats:named-content content-type=\"genus-species\">Brucella<\/jats:named-content>species can cause brucellosis, a zoonotic disease that causes serious livestock economic losses and represents a public health threat. The mechanism of virulence of<jats:named-content content-type=\"genus-species\">Brucella<\/jats:named-content>spp. is not yet fully understood. Therefore, it is crucial to identify new molecules that serve as virulence factors to better understand this host-pathogen interplay. Here, we evaluated the role of the<jats:named-content content-type=\"genus-species\">Brucella<\/jats:named-content>membrane fusogenic protein (Mfp) and outer membrane protein 19 (Omp19) in bacterial pathogenesis. In this study, we showed that<jats:named-content content-type=\"genus-species\">B. abortus<\/jats:named-content>\u0394<jats:italic>mfp<\/jats:italic>::<jats:italic>kan<\/jats:italic>and \u0394<jats:italic>omp19<\/jats:italic>::<jats:italic>kan<\/jats:italic>deletion mutant strains have reduced persistence<jats:italic>in vivo<\/jats:italic>in C57BL\/6 and interferon regulatory factor 1 (IRF-1) knockout (KO) mice. Additionally, 24 h after macrophage infection with a \u0394<jats:italic>mfp<\/jats:italic>::<jats:italic>kan<\/jats:italic>or \u0394<jats:italic>omp19<\/jats:italic>::<jats:italic>kan<\/jats:italic>strain expressing green fluorescent protein (GFP) approximately 80% or 65% of<jats:named-content content-type=\"genus-species\">Brucella<\/jats:named-content>-containing vacuoles (BCVs) retained the late endosomal\/lysosomal marker LAMP-1, respectively, whereas around 60% of BCVs containing wild-type S2308 were found in LAMP-1-negative compartments.<jats:named-content content-type=\"genus-species\">B. abortus<\/jats:named-content>\u0394<jats:italic>omp19<\/jats:italic>::<jats:italic>kan<\/jats:italic>was attenuated<jats:italic>in vivo<\/jats:italic>but had a residual virulence in C57BL\/6 and IRF-1 KO mice, whereas the \u0394<jats:italic>mfp<\/jats:italic>::<jats:italic>kan<\/jats:italic>strain had a lower virulence in these same mouse models. Furthermore, \u0394<jats:italic>mfp<\/jats:italic>::<jats:italic>kan<\/jats:italic>and \u0394<jats:italic>omp19<\/jats:italic>::<jats:italic>kan<\/jats:italic>strains were used as live vaccines. Challenge experiments revealed that in C57BL\/6 and IRF-1 KO mice, the \u0394<jats:italic>mfp<\/jats:italic>::<jats:italic>kan<\/jats:italic>strain induced greater protection than the vaccine RB51 and protection similar that of vaccine S19. However, a \u0394<jats:italic>omp19<\/jats:italic>::<jats:italic>kan<\/jats:italic>strain induced protection similar to that of RB51. Thus, these results demonstrate that<jats:named-content content-type=\"genus-species\">Brucella<\/jats:named-content>Mfp and Omp19 are critical for full bacterial virulence and that the \u0394<jats:italic>mfp<\/jats:italic>::<jats:italic>kan<\/jats:italic>mutant may serve as a potential vaccine candidate in future studies.<\/jats:p>","DOI":"10.1128\/iai.02790-14","type":"journal-article","created":{"date-parts":[[2015,2,3]],"date-time":"2015-02-03T07:53:10Z","timestamp":1422949990000},"page":"1458-1464","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":16,"title":["Mutant Brucella abortus Membrane Fusogenic Protein Induces Protection against Challenge Infection in Mice"],"prefix":"10.1128","volume":"83","author":[{"given":"Job Alves","family":"de Souza Filho","sequence":"first","affiliation":[{"name":"Departamento de Bioqu\u00edmica e Imunologia, Universidade Federal de Minas Gerais, Belo Horizonte, MG, 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