{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T15:21:45Z","timestamp":1790695305457,"version":"4.1.0"},"reference-count":39,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2015,3,1]],"date-time":"2015-03-01T00:00:00Z","timestamp":1425168000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2015,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content content-type=\"genus-species\">Coxiella burnetii<\/jats:named-content>\n            causes human Q fever, a zoonotic disease that presents with acute flu-like symptoms and can result in chronic life-threatening endocarditis. In human alveolar macrophages,\n            <jats:named-content content-type=\"genus-species\">C. burnetii<\/jats:named-content>\n            uses a Dot\/Icm type IV secretion system (T4SS) to generate a phagolysosome-like parasitophorous vacuole (PV) in which to replicate. The T4SS translocates effector proteins, or substrates, into the host cytosol, where they mediate critical cellular events, including interaction with autophagosomes, PV formation, and prevention of apoptosis. Over 100\n            <jats:named-content content-type=\"genus-species\">C. burnetii<\/jats:named-content>\n            Dot\/Icm substrates have been identified, but the function of most remains undefined. Here, we identified a novel Dot\/Icm substrate-encoding open reading frame (CbuD1884) present in all\n            <jats:named-content content-type=\"genus-species\">C. burnetii<\/jats:named-content>\n            isolates except the Nine Mile reference isolate, where the gene is disrupted by a frameshift mutation, resulting in a pseudogene. The CbuD1884 protein contains two transmembrane helices (TMHs) and a coiled-coil domain predicted to mediate protein-protein interactions. The C-terminal region of the protein contains a predicted Dot\/Icm translocation signal and was secreted by the T4SS, while the N-terminal portion of the protein was not secreted. When ectopically expressed in eukaryotic cells, the TMH-containing N-terminal region of the CbuD1884 protein trafficked to the endoplasmic reticulum (ER), with the C terminus dispersed nonspecifically in the host cytoplasm. This new Dot\/Icm substrate is now termed ElpA (\n            <jats:underline>E<\/jats:underline>\n            R-\n            <jats:underline>l<\/jats:underline>\n            ocalizing\n            <jats:underline>p<\/jats:underline>\n            rotein\n            <jats:underline>A<\/jats:underline>\n            ). Full-length ElpA triggered substantial disruption of ER structure and host cell secretory transport. These results suggest that ElpA is a pathotype-specific T4SS effector that influences ER function during\n            <jats:named-content content-type=\"genus-species\">C. burnetii<\/jats:named-content>\n            infection.\n          <\/jats:p>","DOI":"10.1128\/iai.02855-14","type":"journal-article","created":{"date-parts":[[2015,1,21]],"date-time":"2015-01-21T06:01:14Z","timestamp":1421820074000},"page":"1190-1198","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":26,"title":["Identification of ElpA, a Coxiella burnetii Pathotype-Specific Dot\/Icm Type IV Secretion System Substrate"],"prefix":"10.1128","volume":"83","author":[{"given":"Joseph G.","family":"Graham","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, University of Arkansas for Medical Sciences, Little Rock, Arkansas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caylin G.","family":"Winchell","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Arkansas for Medical Sciences, Little Rock, Arkansas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Uma M.","family":"Sharma","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Arkansas for Medical Sciences, Little Rock, Arkansas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel E.","family":"Voth","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Arkansas for Medical Sciences, Little Rock, Arkansas, 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